Store at 2-8°C
71824-20ML 20 ml 10X Wash Buffer Store at 2-8°C
71823-25ML 25 ml 5X Assay Diluent Store at 2-8°C
71871-1PKG 1 PKG 96-well Filter Plate and Polyethylene Plate Sealer Store at 2-8°C
) replicates and
calculating the corresponding concentration.
Linearity
A sample containing 9000 pg/mL TXB2 was diluted
eight times 1:2 in the kit Assay Buffer and measured in
the assay. The data
minutes at 4°C.
7. Transfer the lysate to a 96-well filter plate that has been pre-wetted with 5 µL
Lysis Buffer/ well.
8. Place a low protein binding, 96-well round bottom or V-bottom plate underneath
without making bubbles.
8. Transfer the lysate to a 96-well filter plate that has been pre-wetted with 5 L
Lysis Buffer/ well.
9. Place a low protein binding, 96-well round bottom or V-bottom