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D Hu et al.
The Journal of biological chemistry, 275(4), 2705-2712 (2000-01-25)
The PI-SceI protein is an intein-encoded homing endonuclease that initiates the mobility of its gene by making a double strand break at a single site in the yeast genome. The PI-SceI protein splicing and endonucleolytic active sites are separately located
H Kitagawa et al.
Biochemical and biophysical research communications, 194(1), 375-382 (1993-07-15)
Here we report the cDNA sequence of human Gal beta 1,3(4)GlcNAc alpha 2,3-sialyltransferase. The cDNA was isolated from a human placental cDNA library by screening with a 150bp probe generated by PCR using degenerate primers based on the sequences found
Chloe E H Scott et al.
The bone & joint journal, 102-B(6), 716-726 (2020-06-02)
This study aims to determine the proportion of patients with end-stage knee osteoarthritis (OA) possibly suitable for partial (PKA) or combined partial knee arthroplasty (CPKA) according to patterns of full-thickness cartilage loss and anterior cruciate ligament (ACL) status. A cross-sectional
María Benlloch-Tinoco et al.
Food chemistry, 187, 254-262 (2015-05-16)
The impact of microwave (1000 W - 340 s) and conventional heat (97 °C - 30s) pasteurisation and storage (4, 10, 22 °C for up to 63 d) on total and individual carotenoids and chlorophylls in kiwifruit puree was evaluated.
Luis A Berrueta et al.
Talanta, 187, 330-336 (2018-06-02)
The phenolic profiles of apple cultivars from the SERIDA Asturian cider apple breeding program, including parents and progenies, were determined by ultrahigh-performance liquid chromatography-diode array detector-electrospray ionization-quadrupole time of flight/mass spectrometer in order to study the relationship between phenols and
R Wagener et al.
FEBS letters, 438(3), 165-170 (1998-11-25)
We have recently cloned a cDNA for mouse matrilin-4. By sequence comparison we identified the 12 kb long human matrilin-4 gene as a part of a high-throughput genomic sequence (HS453C12) in the databases. Additionally we found a human matrilin-4 expressed
Danette J Nicolay et al.
Cellular and molecular neurobiology, 24(3), 357-366 (2004-06-23)
1. Although recent advances have provided insight into the transcriptional control of oligodendrocyte (OG) development, little information exists on the role of clustered Hox genes in this process. The aim of this study was to examine the expression profile of
Fatma Gassara-Chatti et al.
Food chemistry, 137(1-4), 18-24 (2012-12-04)
The thermal stability, physico-chemical properties and effect on juice clarification of hydrogel formulations of ligninolytic enzymes from Phanerochaete chrysosporium were evaluated. The results showed that enzyme entrapment increase significantly (P<0.05) the thermal stability of enzymes at 4 and 75 °C.
Marta E Álvarez-Argüelles et al.
Journal of medical virology, 89(12), 2224-2229 (2017-07-07)
This study investigates the presence of Merkel cell polyomavirus (MCPyV) in skin lesions of patients with Merkel cell carcinoma (MCC). MCPyV was quantified using quantitative Real-Time-PCR (qRT-PCR) in 34 paraffinized MCC samples (resected/biopsied) originally taken between 1977 and 2015, and
Jacklyn K Jackson et al.
The British journal of nutrition, 121(7), 831-838 (2019-01-24)
The consumption of nitrate-rich vegetables can acutely lower blood pressure and improve mediators shown to optimise vascular health. However, we do not yet understand the impact of long-term habitual dietary nitrate intake and its association with CVD. Therefore, the aim
Yoshiaki Oshima et al.
Masui. The Japanese journal of anesthesiology, 54(8), 875-880 (2005-08-18)
Continuous epidural administration of droperidol at 2.5 mg x day(-1) or less was performed in 837 patients after surgery. In 4 of these patients, an extrapyramidal reaction occurred. Patient 1 was a 10-year-old girl who developed the symptom 29 hours
M T Huizing et al.
Journal of chromatography. B, Biomedical applications, 674(2), 261-268 (1995-12-15)
A reversed-phase high-performance liquid chromatographic (HPLC) method has been validated for the quantitative determination of the three major paclitaxel metabolites (6 alpha-hydroxypaclitaxel, 3'-p-hydroxypaclitaxel, 6 alpha,3'-p-dihydroxypaclitaxel) in human plasma. The HPLC system consists of an APEX-octyl analytical column and acetonitrile-methanol-0.02 M
Tarayn Fairlie et al.
Obstetrics and gynecology, 121(3), 570-577 (2013-05-03)
To estimate the effectiveness against early-onset group B streptococcal (GBS) disease of intrapartum antibiotic prophylaxis among term and preterm deliveries, deliveries with fewer than 4 hours of antibiotics, and deliveries receiving clindamycin regimens. We performed a secondary analysis of the
J M Anchin et al.
Molecular immunology, 30(16), 1463-1471 (1993-11-01)
A library of monoclonal antibodies (mAb) was made against a trisubstituted guanidinium sweetener (N-(p-cyanophenyl)-N'-(diphenylmethyl)guanidine acetic acid) that is 200,000 times sweeter than sucrose on a molar basis. The mAb were characterized in terms of their ligand affinities, H- and L-chain
Gitte Gotholdt Jensen et al.
Journal of chromatography. A, 1216(27), 5199-5206 (2009-06-03)
An analytical method was developed for the determination of 2,6-dichlorobenzamide (BAM) and five degradation products thereof including 2-chlorobenzamide (OBAM), 2,6-dichlorobenzoic acid (DCBA), 2-chlorobenzoic acid (OBA), benzoic acid (BA) and benzamide (BAD) in water samples. Solid-phase extraction was combined with liquid
Fatemeh Tohidi et al.
Environmental science and pollution research international, 22(15), 11387-11400 (2015-03-27)
A gas chromatography/mass spectrometry (GC/MS)-based method was developed for simultaneous determination of triclosan (TCS) and its degradation products including 2,4-dichlorophenol (2,4-DCP), 2,8-dichlorodibenzo-p-dioxin (2,8-DCDD), and methyl triclosan (MTCS) in wastewater and sludge samples. The method provides satisfactory detection limit, accuracy, precision
F Chen et al.
Chinese medical journal, 113(8), 702-705 (2002-01-05)
To investigate the gene frequencies of 5 major human platelet alloantiqens (HPA 1-5) in Chinese population and to assess if polymorphism of HPA was associated with Chinese acute myocardial infarction (AMI) and acute ischemic stroke (AIS). HPA 1-5 genotyping was
K K Ryckman et al.
Genes and immunity, 10(7), 636-640 (2009-06-26)
Toll-like receptors (TLRs) are critical components of innate immunity, recognizing bacterial microorganisms and initiating local inflammatory responses. In this study, we assessed the impact of genetic variation in TLR genes on cervical concentrations of pro- and anti-inflammatory cytokines, and determined
Annikka Weissferdt et al.
Human pathology, 46(3), 376-383 (2015-01-13)
Primary mediastinal seminomas are unusual tumors that can present in a pure form or as part of a mixed germ cell tumor. Contrary to testicular seminomas, little is known about the expression of novel immunohistochemical markers in mediastinal seminomas. This
J A Encinas et al.
Proceedings of the National Academy of Sciences of the United States of America, 96(5), 2491-2496 (1999-03-03)
The semaphorins comprise a large family of membrane-bound and secreted proteins, some of which have been shown to function in axon guidance. We have cloned a transmembrane semaphorin, Sema W, that belongs to the class IV subgroup of the semaphorin
Tommaso Vincenzo Bartolotta et al.
La Radiologia medica, 119(4), 222-230 (2013-12-04)
This study was undertaken to compare the imaging findings of focal nodular hyperplasia (FNH) in men and women, as seen on multidetector computed tomography (MDCT), magnetic resonance imaging (MRI) and contrast-enhanced ultrasound (CEUS). Two radiologists reviewed 195 imaging studies (17
F Chen et al.
Zhonghua yi xue za zhi, 80(5), 332-335 (2002-01-19)
To investigate the gene frequencies of 5 major human platelets alloantigens (HPA 1 - 5) in Chinese population and to assess if polymorphism of HPA is associated with acute myocardial infarction (AMI) and acute ischemic stroke (AIS) in the Chinese.
Adrian Weisz et al.
Journal of chromatography. A, 1380, 120-129 (2015-01-17)
Three low-level subsidiary color impurities (A, B, and C) often present in batches of the color additive D&C Red No. 33 (R33, Acid Red 33, Colour Index No. 17200) were separated from a portion of R33 by spiral high-speed counter-current
Li Zang et al.
Zhonghua yi xue za zhi, 91(20), 1408-1412 (2011-07-16)
To explore the effect of LRP (leukemia related protein) 16 on insulin resistance in C2-C12 cells and explore its molecular mechanism. Lipidosome transfection and lentivirus mediated siRNA (small interfering RNA) technology were used to establish LRP 16 overexpression and underexpression
Emilia Cantón et al.
Journal of clinical microbiology, 50(12), 3921-3926 (2012-09-28)
The Sensititre YeastOne (SYO) method is a widely used method to determine the susceptibility of Candida spp. to antifungal agents. CLSI clinical breakpoints (CBP) have been reported for antifungals, but not using this method. In the absence of CBP, epidemiological
L Y Yu-Lee et al.
Molecular and cellular biology, 10(6), 3087-3094 (1990-06-01)
The pituitary peptide hormone prolactin (Prl) is a potent inducer of Nb2 T lymphoma cell proliferation. To analyze the early genetic response to the mitogenic signals of Prl, a cDNA library was constructed from Nb2 T cells stimulated for 4
A Martini et al.
Journal of viral hepatitis, 22(10), 800-808 (2015-01-23)
Nonalcoholic steatohepatitis (NASH) enhances the risk of progressive liver disease. In chronic hepatitis C (CHC), liver steatosis is frequent, especially in genotype 3, but its clinical significance is debated. As squamous cell carcinoma antigen (SCCA)-IgM has been associated with advanced
Guadalupe Miró et al.
Parasitology research, 100(2), 317-320 (2006-08-18)
Using routine coprological methods, 1161 faecal samples from animal shelters located in Madrid (Spain) were analysed, showing a 28% prevalence for different intestinal parasites: Giardia duodenalis (7%), Cystoisopora spp. (3.8%), Toxocara canis (7.8%), Toxascaris leonina (6.3%), Ancylostomidae (4%), Trichuris vulpis
Kirsten Vang Nielsen et al.
Breast cancer research and treatment, 127(2), 345-355 (2010-06-18)
The estrogen receptor (ER) is the target of tamoxifen, but endocrine therapies do not benefit all patients with ER positive tumors. We therefore hypothesized that copy number changes in the ESR1 gene, encoding ER, confer resistance. Within a consecutive series
Ewa Stępień et al.
Archives of medical research, 42(5), 375-381 (2011-08-11)
A common single nucleotide polymorphism in the E-selectin (SELE) gene S149R results in the loss of E-selectin ligand binding specificity. The 149R allele has been associated with severe cardiovascular diseases. We hypothesized that S149R may regulate platelet activation after stimuli
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