Revised: 07/26/99
Enzymatic Assay of ββββ-GALACTOSIDASE
(EC 3.2.1.23)
Sigma Prod. No. G-3782
FINAL ASSAY CONCENTRATION:
In a 3.00 ml reaction mix, the final concentrations are 93 mM potassium
Harmonized Standards: EN 61010-1:01
EN 61010-2-010:92
EN 55011:98/A1:99/A2:02
EN 61000-3-2:00+A2:05
EN 61000-3-3:95+A1:01+A2:05
EN 61000-4-2:95 +A1:98 +A2:01
Harmonized Standards: EN 61010-1:01
EN 61010-2-010:92
EN 55011:98/A1:99/A2:02
EN 61000-3-2:00+A2:05
EN 61000-3-3:95+A1:01+A2:05
EN 61000-4-2:95 +A1:98 +A2:01
Harmonized Standards: EN 61010-1:01
EN 61010-2-051:95
EN 55011:98/A1:99/A2:02
EN 61000-3-2:00+A2:05
EN 61000-3-3:95+A1:01+A2:05
EN 61000-4-2:95 +A1:98 +A2:01
3.00 ml reaction mix, the final concentrations are 93 mM sodium phosphate,
2.3 mM o-QLWURSKHQ\O� �'�galactoside, 1.0 mM magnesium chloride, 112 mM 2-mercaptoethanol
DQG������������XQLW� �galactosidase.
3.00 ml reaction mix, the final concentrations are 93 mM sodium phosphate,
2.3 mM o-QLWURSKHQ\O� �'�galactoside, 1.0 mM magnesium chloride, 112 mM 2-mercaptoethanol
DQG������������XQLW� �galactosidase.
3.00 ml reaction mix, the final concentrations are 93 mM sodium phosphate,
2.3 mM o-QLWURSKHQ\O� �'�galactoside, 1.0 mM magnesium chloride, 112 mM 2-mercaptoethanol
DQG������������XQLW� �galactosidase.
3.00 ml reaction mix, the final concentrations are 93 mM sodium phosphate,
2.3 mM o-QLWURSKHQ\O� �'�galactoside, 1.0 mM magnesium chloride, 112 mM 2-mercaptoethanol
DQG������������XQLW� �galactosidase.
3.00 ml reaction mix, the final concentrations are 93 mM sodium phosphate,
2.3 mM o-QLWURSKHQ\O� �'�galactoside, 1.0 mM magnesium chloride, 112 mM 2-mercaptoethanol
DQG������������XQLW� �galactosidase.
1659 (1996).
2. Chong, J.P.J., et al., Nature, 375, 418 (1995).
3. Botchan, M., Proc. Natl. Acad. Sci. USA, 93, 9997
(1996).
4. Romanowski, P., et al., Proc. Natl. Acad. Sci. USA,
93, 10189 (1996
Srinivasula, S.M., et al., Proc. Natl. Acad. Sci. USA,
93, 14486 (1996).
10. Muzio, M., et al., J. Biol. Chem., 272, 2952 (1997).
lpg 9/99
Sigma brand products are sold through Sigma-Aldrich
Soc. 93, 2897 (1971).
2. Hermanson, G.T., Mallia, A.K., and Smith, P.K. In
Immobilized Affinity Ligand Techniques, pp. 69-75,
Academic Press, Inc., San Diego, California (1992).
kat 6/7/99
Product