Plates were incubated at 37°C and 5% CO2.
Medium Changes (Day 2 and beyond)
1. 6-well plate: the mES/iPS Medium Dox (+) and Dox (-) was replaced
every 48 hours.
2.
500 rpm at a shaking diameter of 12.5 mm. The expression is performed
over the course of 24-48 h.
Step 5: Analysis
Before attempting purification, successful expression of your POI can be checked