CN
95-74-9
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关键词:'95-74-9'
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The Effective Use of Protein Kinase Inhibitors
70 20 95 95 78 2 9 2
MAPKAP-K2 99 99 90 5 59 102 98 95 93 97 102 74 90 125 72 98 103 90 97
MSK1 3 57 19 38 81 104 118 86 86
The Effective Use of Protein Kinase Inhibitors
70 20 95 95 78 2 9 2
MAPKAP-K2 99 99 90 5 59 102 98 95 93 97 102 74 90 125 72 98 103 90 97
MSK1 3 57 19 38 81 104 118 86 86
Protocol- EZHS100B-33K
25 61 58.1 95
74 110 104.3 95
222 258 239.4 93
2
0 96.8 - -
25 122 115.8 95
74 171 164.4 96
222
Novabiochem Innovations 02/06
6 12 3 16
HATU/
HOAt
5 9 12 2 8
HBTU 16 11 19 2 7
HBTU/
HOBt
18 12 18 3 3
Table 1: Percentage of deletion peptides observed in the synthesis of
ACP(65-74) (Figure
Novabiochem Innovations 01/12
ACP(65-74) prepared with
SpheriTide® resin.
8 16 24 32
mins
8 16 24 32
mins
Bulletin - 11277081001
linear) double-distilled water to a final volume of 9 µl in a microfuge tube.
Denature the DNA by heating in a boiling water bath for 10 minutes at +95°C and chilling quickly in an ice/water
bath.
Product Information Sheet-11277081001
linear) double-distilled water to a final volume of 9 µl in a microfuge tube.
Denature the DNA by heating in a boiling water bath for 10 minutes at +95°C and chilling quickly in an ice/water
bath.
Product Information Sheet-collh-ro
rats
• Perfusion apparatus
• Sterile forceps
• Sterile beaker
• Culture Vessels
• 50 mm petri dish
• 74 mm filter or 4 sheets sterile gauze
• Perfusion medium, 20x conc.
• Culture medium
• FCS (fetal calf
Microwave accelerated Suzuki couplings employing a polymer-supported phosphine
11 79 70
22 75 65
33 84 74
44 95 82
55 97 75
66 97 67
77 84 80
Fig. 8: HPLC elution profile of 4a.
(HPLC conditions: ACE 3AQ; Gradient:
Novabiochem Innovations 02/04
BBoorroonniicc aacciidd bb
AArryyll--BBrr % Yield % Yield
11 79 70
22 75 65
33 84 74
44 95 82
55 97 75
66 97 67
77 84 80
Merck Biosciences
Product Information Sheet - H0402
Sci. USA,
82(9), 2746-2750 (1985).
15. Brew, S.A., and Ingram, K.C., J. Tissue Culture
Methods, 16, 197-199 (1994).
16. Molinari, A.M. et al., Proc. Nat. Acad. Sci. USA,
74(11), 4886-4890
Data Sheet - P8538
/ml substrate peptide, and trace
[32P]-γ-ATP.
Purity: >95% (SDS-PAGE)
Calculated molecular mass: 77.5 kDa
(apparent molecular mass: 74–79 kDa)3
Precautions and Disclaimer
This product is for
LABORATORY NOTEBOOK ISSUANCE PAGE
15
.9
99
18
.9
98
20
.1
80
4.
00
26
26
.9
82
28
.0
85
30
.9
74
32
.0
6
35
.4
5
39
.9
48
69
.7
23
72
.6
3
74
.9
Lab Notebook Guidelines
15
.9
99
18
.9
98
20
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80
4.
00
26
26
.9
82
28
.0
85
30
.9
74
32
.0
6
35
.4
5
39
.9
48
69
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23
72
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3
74
.9
LABORATORY NOTEBOOK ISSUANCE PAGE
15
.9
99
18
.9
98
20
.1
80
4.
00
26
26
.9
82
28
.0
85
30
.9
74
32
.0
6
35
.4
5
39
.9
48
69
.7
23
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3
74
.9
LABORATORY NOTEBOOK ISSUANCE PAGE
15
.9
99
18
.9
98
20
.1
80
4.
00
26
26
.9
82
28
.0
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.9
74
32
.0
6
35
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5
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.9
48
69
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3
74
.9
Salts for analysis
– – 105819 74
52.8 54.6 57.5 60.7 65.9 72.7 41.2 1.388 (25°C) 105833 74
63.9 70.5 81.8 93.7 110.9 – – – 105853 74
– 50.0 (25°C) – – – – – – 105874 74
Data Sheet - SAB4200805
Immunol. Methods, 111,
1-9 (1988).
9. Chiu, N.H.L., and Watson, A.L., Curr. Protoc.
Toxicol., 74, 4.44.1-4.44.8 (2017).
10. Hayashibe, K. et al., J. Immunoassay, 11, 89-95
(1990).
SG,DR
Product Information Sheet - P7626
,24 or
• anhydrous (100%, not 95%) ethanol25
Storage/Stability
A 200 mM PMSF solution in dry solvent has been
reported to remain active for at least 9 months at
Product Information Sheet - 08095
; Chem.
Abstr. 1980, 92, 90266t.
8. Miyashita, Y. et al. Japan Kokai 74 23 693, 1974; Chem. Abstr. 1974,
81, 60501w.
9. Avigad, G. Anal. Biochem. 1983, 134, 499.
10. Lambert, J.; Chiang, Y. U.S
PCR Protocols and Guides - Simplify your gene discovery
IGFR2 receptor target
94 °C 2 min
98 °C 10 s, 74 °C 9 min (5 cycles)
98 °C 10 s, 72 °C 9 min (5 cycles)
98 °C 10 s, 70 °C 9 min (5 cycles)
98 °C 10 s, 68 °C 9 min
Bulletin -162892
; Chem.
Abstr. 1980, 92, 90266t.
8. Miyashita, Y. et al. Japan Kokai 74 23 693, 1974; Chem. Abstr. 1974,
81, 60501w.
9. Avigad, G. Anal. Biochem. 1983, 134, 499.
10. Lambert, J.; Chiang, Y. U.S
Anti-Bcl-xL and Bcl-xS antibody produced in rabbit (B6054)
4521-
4529 (1999).
2. Clem, R.J., et al., Proc. Natl. Acad. Sci. USA, 95,
554 (1998).
3. Boise, L.H., et al., Cell, 74, 597 (1993).
kaa/lpg 01/04
Sigma brand products are sold
Data Sheet - A6941
equal 74 kDa subunits; each containing a flavin
adenine dinucleotide (FAD) molecule.
2
Cofactor:
2
FAD, one molecule/subunit
Isoelectric point:
8
5.4–5.8
pH Range:
9
6.5
Data Sheet - SRP5049
glutathione, 0.1 mM EDTA,
0.25 mM DTT, 0.1 mM PMSF and 25% glycerol.
Molecular mass: ∼74 kDa
Purity: 70–95% (SDS-PAGE, see Figure 1)
Specific Activity: 39–53 nmole/min/mg (see Figure 2)
Precautions
Data Sheet - P1056
701, 38297 St Quentin
Fallavier, Cedex, France * Numéro Vert 05 21 14 08 Téléphone: 74 82
28 00 * Télécopier: 74 95 68 08
SIGMA QUIMICA, Apt. Correos 161, 28100 Alcobendas (Madrid)
ESPAÑA * Telephone
Impurities & Related Compounds: Reference Materials
-17-2
1367567-95-0
71786-67-9
1094089-46-9
57-41-0
3060-50-2
6802-95-5
6859-11-6
630-93-3
68890-66-4
191217-81-9
106092-09-5
104632-27-1
55268-74-1
50-24-8
52
Specialty Enzyme Product Listing
incorporates 10 nmol of total
deoxyribonucleoside triphosphates into acid precipitable DNA
in 30 minutes at 74 °C
Storage: –20 °C
Shipped in wet ice
Cat. No. Product Description Quantity
D7442 MTP Taq
MMP Activation Chart
M75RTPR 1x
Wilhelm et al., 1989,
Imai,JBC270/'95/6691; Okada,
JBC267,'92, 21712; Shapiro JBC
270,1995,6351
TrypsinTPCK, 10 µg/ml 37°C, 2h B 74 & 68 64 66.6*
A74MRTPR; C-term
cleavage 1x
Monoclonal Anti-Bcl-2 antibody produced in mouse (B9804)
2239 (1996).
9. Wolter, K.G., et al., J. Cell Biol., 139, 1281 (1997).
10. Antonsson, B., et al., Science, 277, 370 (1997).
11. Jurgensmeier, J.M., et al., Proc. Natl. Acad. Sci.
USA, 95, 4997 (1998
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