and inhibit initial adsorption to susceptible cells. 25
References:
1. Rankin, J.C. and Jeanes, A., J. Am. Chem. Soc., 76, 4435 (1954).
2. Dimler, R.J. et al., J. Am. Chem. Soc., 77, 6568 (1955).
P., Role of
mammalian RNase Inhibitor in cell-free protein
synthesis. Proc. Natl. Acad. Sci. USA, 76(10),
1898-1902 (1979).
5. Short Protocols in Molecular Biology, 4th ed.,
Ausubel, F. M., et al
mM EDTA,
1 mM 2-mercaptoethanol, 100 mM NaCl, 10 units/ml
aprotinin, and 50% glycerol.
Purity: ≥95% (SDS-PAGE)
Specific activity: ≥1.5 units/mg protein
Unit Definition: One unit will phosphorylate
P., Role of
mammalian RNase Inhibitor in cell-free protein
synthesis. Proc. Natl. Acad. Sci. USA, 76,
4898-4902 (1979).
5. Short Protocols in Molecular Biology, 4th ed.,
Ausubel, F. M., et al.
should retain activity for two years.
References
1. Gibon, Y. et al., Plant J., 30(2), 221-235 (2002).
2. Opperdoes, P. et al., Eur. J. Biochem., 76(1), 29-39
(1977).
3. Streitenberger, S.A. et.
total protein compared
to crude samples.
References
1. Laemmli, U.K., Nature, 227, 680 (1970).
2. Towbin, H. et al., Proc. Natl. Acad. Sci. USA, 76,
4350 (1979).
ColorBurst is a trademark of Sigma-Aldrich
should retain activity for two years.
References
1. Gibon, Y. et al., Plant J., 30(2), 221-235 (2002).
2. Opperdoes, P. et al., Eur. J. Biochem., 76(1), 29-39
(1977).
3. Esders, T.W., and Michrina
we recommend
determining optimal working dilutions by titration.
References
1. Beitzinger, M., et al., RNA Biol., 4, 76-84 (2007).
2. Hock, J., and Meister, G., Genome Biol., 9,
210.1–210.8 (2008
References
1. Jones, K.R. et al., Targeted disruption of the BDNF
gene perturbs brain and sensory neuron
development but not motor neuron development.
Cell, 76, 989 (1994).
2. Snider
ng/ml.
The purity of recombinant mouse TRAIL R2/ Fc
chimera is >95% determined by SDS-PAGE with silver
staining.
References
1. Wu, G.S., et al., Cancer Research, 59, 2770-2775
(1999).
2. Chaudhary
.1 mM EDTA,
0.25 mM DTT, 0.1 mM PMSF, and 25% glycerol.
Molecular mass: ∼76 kDa
Purity: 70–95% (SDS-PAGE, see Figure 1)
Specific Activity: 9.4–12.6 nmole/min/mg (see Figure 2)
Precautions and
. Curr. Heart Fail. Rep., 11(1),
58–63 (2014).
3. Verdecchia, P. et al., The pivotal link between
ACE2 deficiency and SARS-CoV-2 infection.
Eur. J. Intern. Med., 76, 14-20 (2020).
4. Hoffmann