at least 10 µg/ml using A431
cells fixed with 95% ethanol and 5% acetic acid.
By immunoprecipitation, 4 µg will immunoprecipitate
STAT3 from 0.5-1 mg of an EGF stimulated human
A431 cell lysate.
repeated
freeze/thaw cycles.
RELATED
REFERENCES:
1. Sakurai, T et al. (1998) Cell. 92:573-585.
2. DeLecca, L et al. (1998) PNAS. 95:322-327.
Important Note: During shipment, small volumes
faint yellow powder
Molecular weight: 174.2 g/mol
Molecular Formula: C7H7FO2S
Melting Point: 92-93°C
Store at room temperature
PMSF is an inhibitor of serine proteases and acetylcholinesterase
months. Avoid repeated freeze/thaw
cycles.
REFERENCES: 1. Sakurai, T et al. (1998) Cell. 92:573-585.
2. DeLecca, L et al. 91998) PNAS. 95:322-327.
Important Note: During shipment, small volumes
STORAGE AND STABILITY
Solid MCPBA shows less that 1% decomposition when stored
at room temperature for 1 year. It has been determined that 95-100%
metarial can be detonated by shock or sparks. 85%
Cancer, 47, 811-6
(1991).
10. Borsi, L. et al., Int. J. Cancer, 52 688-92 (1992).
11. Castellucci, M. et al., Histochemistry, 95, 449-58
(1991).
12. Balza, E. et al., FEBS Lett., 332, 39-43 (1993
as previously described (Belkin, 1990).
PURITY:
> 95%: Nonreduced 7.5% PAAG stained with Silver stain shows major bands at 92 kDa
(beta3 subunit), 145 kDa (alphaV subunit) and a minor band
as previously described (Belkin, 1990).
PURITY:
> 95%: Nonreduced 7.5% PAAG stained with Silver stain shows major bands at 92 kDa
(beta3 subunit), 145 kDa (alphaV subunit) and a minor band
98% (HPLC)
A4262 Actinomycin D 95%
Lyophilized powder with a purity of 95% (HPLC)
A9415 Actinomycin D,
cell culture tested
Lyophilized powder with purity of 95% (HPLC). For cell
culture applications
98% (HPLC)
A4262 Actinomycin D 95%
Lyophilized powder with a purity of 95% (HPLC)
A9415 Actinomycin D,
cell culture tested
Lyophilized powder with purity of 95% (HPLC). For cell
culture applications
98% (HPLC)
A4262 Actinomycin D 95%
Lyophilized powder with a purity of 95% (HPLC)
A9415 Actinomycin D,
cell culture tested
Lyophilized powder with purity of 95% (HPLC). For cell
culture applications
98% (HPLC)
A4262 Actinomycin D 95%
Lyophilized powder with a purity of 95% (HPLC)
A9415 Actinomycin D,
cell culture tested
Lyophilized powder with purity of 95% (HPLC). For cell
culture applications
PMSF, and 25% glycerol.
Molecular mass: ∼71 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 92–124 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product is for R&D
uncoupling protein (1). It is only 59%
homologous with UCP1 found in brown adipose tissues. Mouse UCP2 (chromosome 7)
has approximately 95% homology with human UCP2 located on chromosome 11 (1).
UCP2 has
with a
micropipette.
4. Add 0.1 volume of 3M Sodium Acetate (pH 5.2) and 1 volume of isopropanol or 2.5 volumes of
95% ethanol. Mix and place on ice for 2–5 minutes. Spin at top speed in a
repeated freeze/thaw cycles.
RELATED
REFERENCES:
1. Sakurai, T et al. (1998) Cell. 92:573-585.
2. DeLecca, L et al. (1998) PNAS. 95:322-327.
Important Note: During shipment, small volumes