10:19 AM1
2 Montage SEQ96 Sequencing Reaction Cleanup Kit User Guide
Kit Components
The following parts are included in the Montage SEQ
96
Sequencing Reaction
Cleanup Kit:
■ 96 well
sigma-aldrich.com
5-Bromo-2’-deoxy-uridine
Labeling and Detection Kit III
For life science research only.
Not for use in diagnostic procedures.
y Version: 19
Content Version: December 2020
96-well microplate
Plate
Can be any 96-well plate, regular or
conical welled.
Diagram of Centrifuge Align-
ment Frame Assembly
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XX
A
D
C
B
PR02732_RevB.indd 1 5/27/2008 8:21:19 AM
250 µL of growth
medium (for 24-well or 96-well assays, respectively)
were added to each receiver plate well. HUVEC were
cultured in a 37 °C / 5% CO2 tissue culture incubator
for
and 2 for concentrations tested) in binding buffer in a
nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest
plate (Millipore cat. # MAHF C1H) is coated with 0.33%
sample is transferred to a 96 well plate (for
LC/MS analysis), followed by the addition of 20 µl of 1x QND internal
standard (250 ng/ml, diluted from Vial F).
19. The samples
Sufficient for 100 tests in 96-well plates
Reagents and Equipment Required but Not
Provided.
Pipetting devices and accessories
(e.g., multichannel pipettor)
Black flat bottom 96-well plates
Fluorescence
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Version 1.2 Page 19
4. If all wells (except A1-A4, B1-B4, C1-C4) are dark green after color
development, probably you did not add Blocker solution to the
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2. How to Use this Product
2.1. Before you Begin
Sample Materials
The Cytotoxicity Detection Kit (LDH) is used with cell-free supernatants obtained from cells cultured in 96-well
microplates
tests in 96-well plates
Reagents and Equipment Required but Not
Provided.
Pipetting devices and accessories (e.g.,
multichannel pipettor)
Black flat bottom 96-well plates
Covid-19.
Covidyte TF670 is a peptide substrate containing a
14-amino acid sequence (KTSAVLQSGFRKME) that
can be cleaved by coronavirus proteases. The dark-
FRET peptide contains Tide Quencher™ 5 (TQ5