seeding density for the culture of interest.
2. Prepare the 96 well plate by removing it from its
packaging in aseptic conditions.
3. Pipette 100 µl of cells and medium at the
appropriate seeding
Mg K P Na
A7002 Agar 33-35°C 7.0-7.6 2-4% 0.30 0.10 0.01 0.01 0.50 (%)
A6549 Agar, Type A 33-35°C 7.0-8.0 5-6% N/A N/A N/A N/A N/A
A6674 Agar, Type E 33-35°C 7.0-7.8 3-4% 0.02 0.02
Mg K P Na
A7002 Agar 33-35°C 7.0-7.6 2-4% 0.30 0.10 0.01 0.01 0.50 (%)
A6549 Agar, Type A 33-35°C 7.0-8.0 5-6% N/A N/A N/A N/A N/A
A6674 Agar, Type E 33-35°C 7.0-7.8 3-4% 0.02 0.02
Mg K P Na
A7002 Agar 33-35°C 7.0-7.6 2-4% 0.30 0.10 0.01 0.01 0.50 (%)
A6549 Agar, Type A 33-35°C 7.0-8.0 5-6% N/A N/A N/A N/A N/A
A6674 Agar, Type E 33-35°C 7.0-7.8 3-4% 0.02 0.02
Mg K P Na
A7002 Agar 33-35°C 7.0-7.6 2-4% 0.30 0.10 0.01 0.01 0.50 (%)
A6549 Agar, Type A 33-35°C 7.0-8.0 5-6% N/A N/A N/A N/A N/A
A6674 Agar, Type E 33-35°C 7.0-7.8 3-4% 0.02 0.02
Mg K P Na
A7002 Agar 33-35°C 7.0-7.6 2-4% 0.30 0.10 0.01 0.01 0.50 (%)
A6549 Agar, Type A 33-35°C 7.0-8.0 5-6% N/A N/A N/A N/A N/A
A6674 Agar, Type E 33-35°C 7.0-7.8 3-4% 0.02 0.02
Mg K P Na
A7002 Agar 33-35°C 7.0-7.6 2-4% 0.30 0.10 0.01 0.01 0.50 (%)
A6549 Agar, Type A 33-35°C 7.0-8.0 5-6% N/A N/A N/A N/A N/A
A6674 Agar, Type E 33-35°C 7.0-7.8 3-4% 0.02 0.02
Mg K P Na
A7002 Agar 33-35°C 7.0-7.6 2-4% 0.30 0.10 0.01 0.01 0.50 (%)
A6549 Agar, Type A 33-35°C 7.0-8.0 5-6% N/A N/A N/A N/A N/A
A6674 Agar, Type E 33-35°C 7.0-7.8 3-4% 0.02 0.02
Mg K P Na
A7002 Agar 33-35°C 7.0-7.6 2-4% 0.30 0.10 0.01 0.01 0.50 (%)
A6549 Agar, Type A 33-35°C 7.0-8.0 5-6% N/A N/A N/A N/A N/A
A6674 Agar, Type E 33-35°C 7.0-7.8 3-4% 0.02 0.02
Mg K P Na
A7002 Agar 33-35°C 7.0-7.6 2-4% 0.30 0.10 0.01 0.01 0.50 (%)
A6549 Agar, Type A 33-35°C 7.0-8.0 5-6% N/A N/A N/A N/A N/A
A6674 Agar, Type E 33-35°C 7.0-7.8 3-4% 0.02 0.02
present.
Components
The kit is sufficient for 100 assays in 96 well plates.
Reagent A 5 mL
Catalog Number MAK131A
Reagent B 3 mL
Catalog Number MAK131B
10% TCA 5 mL
Catalog Number MAK131C
conformations analysed by combinatorial
mutations and enantiomeric phosphonates.
Biochem. J., 373, 33–40 (2003).
3. Ordentlich, A. et al. The Architecture of Human
Acetylcholinesterase Active Center Probed
antitumor activity and drug resistance have been
reported.9,16,19,34-36
T5648 Page 3 of 3
10/30/96 - ARO
TAMOXIFEN FREE BASE
Sigma Prod. No. T5648
GENERAL NOTES: (continued)
Studies
and aspirate 10 μL of 3.3% Formic acid
(pH=2.0), then dispense.
3. Repeat step 2 - 3X.
Product code: 54263-U (96 pk)
Store at Room Temperature
4. Draw 10 μL