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A Weinberg et al.
Journal of clinical microbiology, 34(2), 445-446 (1996-02-01)
We evaluated an enzyme-linked immunoassay (EIA; BioWhittaker) and a latex agglutination (LA; Becton Dickinson) for varicella-zoster virus (VZV) antibody determination, using cell-mediated immunity (CMI) as a "gold standard." VZV EIA had a sensitivity, specificity, positive predictive value, and negative predictive
J D Tefft et al.
Current biology : CB, 9(4), 219-222 (1999-03-13)
In Drosophila embryos, the loss of sprouty gene function enhances branching of the respiratory system. Three human sprouty homologues (h-Spry1-3) have been cloned recently, but their function is as yet unknown [1]. Here, we show that a murine sprouty gene
Steven G Carmella et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 935, 36-40 (2013-08-13)
3-Hydroxypropylmercapturic acid (3-HPMA) and 3-hydroxy-1-methylpropylmercapturic acid (HMPMA) are urinary metabolites of the toxicants acrolein and crotonaldehyde, respectively. Virtually all human urine samples contain these metabolites, resulting from the action of glutathione-S-transferases on acrolein and crotonaldehyde, which are lipid peroxidation products
W Yang et al.
DNA sequence : the journal of DNA sequencing and mapping, 12(4), 239-251 (2002-03-28)
The actions of platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) are mediated through the PAF receptor (PAFr), which is a member of G-protein coupled superfamily of receptors. Our laboratory has data showing PAF has a role(s) in reproduction in domestic animals. Porcine, bovine
Hadi Yaziji et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 19(4), 514-523 (2006-03-24)
We evaluated the sensitivity and specificity of 10 monoclonal and two polyclonal antibodies for distinguishing epithelioid mesothelioma from adenocarcinoma (AdCA) using immunohistochemistry (IHC). The antibodies were directed against the mesothelial-associated antigens mesothelin, calretinin, cytokeratin 5, thrombomodulin, Wilms' tumor-1 (WT-1) gene
Zhong-Liu Wu et al.
Biotechnology and applied biochemistry, 35(Pt 1), 61-67 (2002-02-09)
Racemic 2-phenylpropionitrile was resolved enantioselectively by nitrile-converting enzymes in cells of Rhodococcus sp. CGMCC 0497 to S-(+)-2-phenylpropionic acid and R-(-)-2-phenylpropionamide. By optimization of the culture conditions, great enhancement of enzyme activity and enantioselectivity was achieved. Furthermore, the relationship between cell-growth
Johnathan M Sheele et al.
Tropical medicine & international health : TM & IH, 18(4), 477-484 (2013-01-22)
To determine whether the detection of human IgG bound to Schistosoma haematobium eggs from filtered urine could be used as a rapid diagnostic test (RDT-Sh). We filtered 160 urine samples from children in the Kwale District of Kenya to isolate
Linda Gergen et al.
Avian pathology : journal of the W.V.P.A, 48(1), 45-56 (2018-11-09)
A double recombinant strain of herpes virus of turkeys (HVT) was constructed that contains the fusion (F) gene from Newcastle disease virus (NDV) and the gD plus gI genes from infectious laryngotracheitis virus (ILTV) inserted into a non-essential region of
J E King et al.
Histopathology, 48(3), 223-232 (2006-01-25)
Immunohistochemistry is frequently employed to aid the distinction between mesothelioma and pulmonary adenocarcinoma metastatic to the pleura, but there is uncertainty as to which antibodies are most useful. We analysed published data in order to establish sensitivity and specificity of
Hyon Jae Lee et al.
Annals of the New York Academy of Sciences, 1043, 135-145 (2005-07-23)
The activity of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) can play an important role in regulating multiple upstream pathways relating to the development of diabetic complications. GAPDH can be modified by a number of metabolic factors, including oxidative and glycation products. To study
Hyun-Jung Jang et al.
AJR. American journal of roentgenology, 201(2), 314-321 (2013-07-26)
The purpose of this study was to identify the essential number of phases from multiphasic CT for 1- to 2-cm hepatocellular carcinoma (HCC) on surveillance ultrasound and to compare the results with the American Association for the Study of Liver
Michael Schmieder et al.
Neoplasia (New York, N.Y.), 10(10), 1154-1162 (2008-09-25)
Gastrointestinal stromal tumors (GISTs) are characterized by alterations in genes involved in cell cycle regulation. Although p16 (INK4A) have been extensively investigated in GISTs, there are still discrepancies regarding its prognostic value. Therefore, we evaluated the clinical occurrence, diagnostic and
A Caron et al.
Domestic animal endocrinology, 72, 106408-106408 (2020-02-03)
The objective of this project was to determine the effects of sustained hyperprolactinemia for 7 or 20 d on mammary development in late-pregnant gilts. On day 90 of gestation, gilts were assigned to one of 3 groups to receive intramuscular (IM)
Niek E G Beurskens et al.
The international journal of cardiovascular imaging, 33(11), 1723-1730 (2017-06-02)
Quantification of pulmonary regurgitation (PR) is essential in the management of patients with repaired tetralogy of Fallot (TOF). We sought to evaluate the accuracy of first-line Doppler echocardiography in comparison with cardiac magnetic resonance imaging (MRI) to identify hemodynamic significant
EunChae Ryu et al.
Environmental science and pollution research international, 28(6), 7551-7563 (2020-10-11)
A simplified method is described for reducing the analysis time of nitrofurans (NFs) and nitrofuran metabolites (NFMs) in the aquatic animals. Most existing HPLC-MS/MS methods are intended only for NFMs and are based on their fast metabolic transformations. We optimized
C Grunau et al.
Nucleic acids research, 29(13), E65-E65 (2001-07-04)
Bisulfite genomic sequencing is the method of choice for the generation of methylation maps with single-base resolution. The method is based on the selective deamination of cytosine to uracil by treatment with bisulfite and the sequencing of subsequently generated PCR
F Lai et al.
Genomics, 22(3), 610-616 (1994-08-01)
Since the realization that the vesicular transport machinery is conserved from yeast to vertebrate neurons, much interest in the proteins involved has been generated. Here we describe a new type of mammalian YPT/Rab protein, named Rab11b, that is most abundantly
Farid W van der Mei et al.
Journal of the American Chemical Society, 139(26), 9053-9065 (2017-06-27)
A practical method for enantioselective synthesis of fluoroalkyl-substituted Z-homoallylic tertiary alcohols has been developed. Reactions may be performed with ketones containing a polylfluoro-, trifluoro-, difluoro-, and monofluoroalkyl group along with an aryl, a heteroaryl, an alkenyl, an alkynyl, or an
Thomas A Moss et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 19(9), 3071-3081 (2013-01-22)
An efficient catalytic and stereoselective method for the direct construction of protected ethylene-amino and propylene-amino scaffolds attached to quaternary stereocentres is reported. Preliminary investigations revealed a mild base catalysed nucleophilic ring opening of N-sulfonyl aziridines using the commercially available phosphazene
T Hiratsuka
Biochemistry, 26(11), 3168-3173 (1987-06-02)
When myosin subfragment 1 (S-1) reacts with the bifunctional reagents with cross-linking spans of 3-4.5 A, p-nitrophenyl iodoacetate and p-nitrophenyl bromoacetate, the 20-kilodalton (20-kDa) segment of the heavy chain is cross-linked to the 26-kDa segment via the reactive thiol SH2.
Ana Lozano et al.
Journal of AOAC International, 101(2), 374-382 (2017-11-17)
This paper presents an efficiency evaluation of GC coupled with quadrupole Orbitrap MS for identification and quantitation in the multiresidue pesticide analysis of baby foods in full-scan mode. The identification criteria were studied following SANTE guidelines (retention time, mass accuracy
R A White et al.
Mammalian genome : official journal of the International Mammalian Genome Society, 3(5), 281-285 (1992-01-01)
Ankyrin is an essential link between cytoskeletal proteins, such as spectrin, and membrane bound proteins, such as protein 3, the erythrocyte anion exchanger. Although the amino acid structure of human ankyrin is known, the functional regions have been only partially
Laura G Henry et al.
Veterinary parasitology, 254, 178-182 (2018-04-17)
Patient-side test kits for detecting antigenemia in dogs associated with sexually mature female heartworms (Dirofilaria immitis) have been available for three decades, and these tests are continually updated and improved. To define the sensitivity (Se) and specificity (Sp) of contemporary
Yun Li et al.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 37(7), 929-933 (2012-07-17)
To establish an HPLC method for simultaneous determination of contents of loureirin A, loureirin B, 7,4'-dihydroxy flavone, pterostilbene and resveratrol in resina draconis and its extracts. Kromasil 100-5C18 column (4.6 mm x 250 mm, 5 microm) was used with the
David Voglar et al.
Environmental technology, 35(9-12), 1389-1400 (2014-04-08)
We demonstrate here, in a pilot-scale experiment, the feasibility of ethylenediaminetetraacetate (EDTA)based washing technology for soils contaminated with potentially toxic metals. Acid precipitation coupled to initial alkaline toxic metal removal and an electrochemical advanced oxidation process were used for average
C Y Gao et al.
Gene, 176(1-2), 243-247 (1996-10-17)
A rat PCTAIRE-1 cDNA clone was isolated by immunoscreening of a PC12 cDNA library, followed by 5' RACE (rapid amplification of cDNA ends) to determine the 5' end. The rat PCTAIRE-1 cDNA sequence is 96% identical to mouse PCTAIRE-1 and
S Hatakeyama et al.
International immunology, 10(5), 631-637 (1998-06-30)
Here we report the genomic cloning and characterization of the murine A1 genes, which belong to the bcl-2 gene family. Southern analysis indicated the existence of at least four A1 genes in the murine genome and four different A1 genes
M A Dombrink-Kurtzman et al.
The Journal of biological chemistry, 264(8), 4513-4522 (1989-03-15)
Previous studies of murine IgM hybridoma protein 18-2-3, derived from an (NZB/NZW)F1 mouse following hyperimmunization with fluorescein (Fl)-conjugated keyhole limpet hemocyanin, demonstrated a high affinity for Fl (Ka = 2.9 x 10(10) M-1) and cryoprecipitation that was abrogated upon Fl
Oscar O Braun et al.
European journal of pharmacology, 592(1-3), 128-132 (2008-07-23)
Platelets constitute a key role in vascular injuries, however, the detailed mechanisms behind platelet-endothelial cell and platelet-leukocyte interactions in the femoral artery are not yet fully elucidated. We used intravital fluorescence microscopy of the femoral artery in C57BL/6 mice to
Ge Xiong et al.
Journal of molecular biology, 386(4), 976-988 (2009-02-27)
The C. elegans gene unc-89 encodes a set of mostly giant polypeptides (up to 900 kDa) that contain multiple immunoglobulin (Ig) and fibronectin type 3 (Fn3), a triplet of SH3-DH-PH, and two protein kinase domains. The loss of function mutant
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