Buffer, pH 5.0 at 37°C (NaOAC)
(Prepare 100 ml in deionized water using Sodium Acetate, Trihydrate, Sigma Prod.
No. S-8625. Adjust to pH 5.0 at 37°C with 5 M HCl.)
D. 0.1% (w/v) 3-Methyl-2-Benzothiazolinone
Page 3 of 3
Revised: 05/04/98
Enzymatic Assay of L-LACTIC DEHYDROGENASE1
(EC 1.1.1.27)
UNIT DEFINITION:
One unit will reduce 1.0 µmole of pyruvate to L-lactate per minute at pH 7.5 at 37°C.
Page 3 of 3
Revised: 05/04/98
Enzymatic Assay of L-LACTIC DEHYDROGENASE1
(EC 1.1.1.27)
UNIT DEFINITION:
One unit will reduce 1.0 µmole of pyruvate to L-lactate per minute at pH 7.5 at 37°C.
Page 3 of 3
Revised: 05/04/98
Enzymatic Assay of L-LACTIC DEHYDROGENASE1
(EC 1.1.1.27)
UNIT DEFINITION:
One unit will reduce 1.0 µmole of pyruvate to L-lactate per minute at pH 7.5 at 37°C.
Page 3 of 3
Revised: 05/04/98
Enzymatic Assay of L-LACTIC DEHYDROGENASE1
(EC 1.1.1.27)
UNIT DEFINITION:
One unit will reduce 1.0 µmole of pyruvate to L-lactate per minute at pH 7.5 at 37°C.
Page 3 of 3
Revised: 05/04/98
Enzymatic Assay of L-LACTIC DEHYDROGENASE1
(EC 1.1.1.27)
UNIT DEFINITION:
One unit will reduce 1.0 µmole of pyruvate to L-lactate per minute at pH 7.5 at 37°C.
Page 3 of 3
Revised: 05/04/98
Enzymatic Assay of L-LACTIC DEHYDROGENASE1
(EC 1.1.1.27)
UNIT DEFINITION:
One unit will reduce 1.0 µmole of pyruvate to L-lactate per minute at pH 7.5 at 37°C.
Page 3 of 3
Revised: 05/04/98
Enzymatic Assay of L-LACTIC DEHYDROGENASE1
(EC 1.1.1.27)
UNIT DEFINITION:
One unit will reduce 1.0 µmole of pyruvate to L-lactate per minute at pH 7.5 at 37°C.
cuvettes and record the A660nm for
Standards and Standard Blank.
SS-ATP05 Page 3 of 3
Revised: 07/29/98
Enzymatic Assay of PYROPHOSPHATASE, ACID
CALCULATIONS:
Standard Curve:
A660nm
Page 3 of 3
Revised: 05/04/98
Enzymatic Assay of L-LACTIC DEHYDROGENASE1
(EC 1.1.1.27)
UNIT DEFINITION:
One unit will reduce 1.0 µmole of pyruvate to L-lactate per minute at pH 7.5 at 37°C.
Page 3 of 3
Revised: 05/04/98
Enzymatic Assay of L-LACTIC DEHYDROGENASE1
(EC 1.1.1.27)
UNIT DEFINITION:
One unit will reduce 1.0 µmole of pyruvate to L-lactate per minute at pH 7.5 at 37°C.
Page 3 of 3
Revised: 05/04/98
Enzymatic Assay of L-LACTIC DEHYDROGENASE1
(EC 1.1.1.27)
UNIT DEFINITION:
One unit will reduce 1.0 µmole of pyruvate to L-lactate per minute at pH 7.5 at 37°C.
Page 3 of 3
Revised: 05/04/98
Enzymatic Assay of L-LACTIC DEHYDROGENASE1
(EC 1.1.1.27)
UNIT DEFINITION:
One unit will reduce 1.0 µmole of pyruvate to L-lactate per minute at pH 7.5 at 37°C.
Page 3 of 3
Revised: 05/04/98
Enzymatic Assay of L-LACTIC DEHYDROGENASE1
(EC 1.1.1.27)
UNIT DEFINITION:
One unit will reduce 1.0 µmole of pyruvate to L-lactate per minute at pH 7.5 at 37°C.
Page 3 of 3
Revised: 05/04/98
Enzymatic Assay of L-LACTIC DEHYDROGENASE1
(EC 1.1.1.27)
UNIT DEFINITION:
One unit will reduce 1.0 µmole of pyruvate to L-lactate per minute at pH 7.5 at 37°C.
Page 3 of 3
Revised: 05/04/98
Enzymatic Assay of L-LACTIC DEHYDROGENASE1
(EC 1.1.1.27)
UNIT DEFINITION:
One unit will reduce 1.0 µmole of pyruvate to L-lactate per minute at pH 7.5 at 37°C.
Page 3 of 3
Revised: 05/04/98
Enzymatic Assay of L-LACTIC DEHYDROGENASE1
(EC 1.1.1.27)
UNIT DEFINITION:
One unit will reduce 1.0 µmole of pyruvate to L-lactate per minute at pH 7.5 at 37°C.
Page 3 of 3
Revised: 05/04/98
Enzymatic Assay of L-LACTIC DEHYDROGENASE1
(EC 1.1.1.27)
UNIT DEFINITION:
One unit will reduce 1.0 µmole of pyruvate to L-lactate per minute at pH 7.5 at 37°C.
Page 3 of 3
Revised: 05/04/98
Enzymatic Assay of L-LACTIC DEHYDROGENASE1
(EC 1.1.1.27)
UNIT DEFINITION:
One unit will reduce 1.0 µmole of pyruvate to L-lactate per minute at pH 7.5 at 37°C.
SPCHOL01 Page 3 of 4
Revised: 03/16/98
Enzymatic Assay of CHOLESTEROL ESTERASE
(EC 3.1.1.13)
PROCEDURES: (continued)
Mix by inversion and equilibrate to 37°C. Then add:
Reagent
SPCHOL01 Page 3 of 4
Revised: 03/16/98
Enzymatic Assay of CHOLESTEROL ESTERASE
(EC 3.1.1.13)
PROCEDURES: (continued)
Mix by inversion and equilibrate to 37°C. Then add:
Reagent
SPCHOL01 Page 3 of 4
Revised: 03/16/98
Enzymatic Assay of CHOLESTEROL ESTERASE
(EC 3.1.1.13)
PROCEDURES: (continued)
Mix by inversion and equilibrate to 37°C. Then add:
Reagent
SPCHOL01 Page 3 of 4
Revised: 03/16/98
Enzymatic Assay of CHOLESTEROL ESTERASE
(EC 3.1.1.13)
PROCEDURES: (continued)
Mix by inversion and equilibrate to 37°C. Then add:
Reagent
swirling and equilibrate 100 µl of the reaction cocktail to 37°C. Keep in a N2 environment.
RSPROT02 Page 3 of 4
Revised: 08/07/98
Enzymatic Assay of CATECHOL-O-METHYL