SDS-PAGE)
with ERK-1 (44 kDa protein) and ERK-2 (42 kDa
protein ) using NIH 3T3 mouse fibroblast cell extract and
rat brain extract. Staining of the 42 and 44 kDa bands is
specifically inhibited with MAP
yellow
Solubility
water. . . soluble, aqueous solutions of pH 4.3-6.2 are stable up to 3
weeks at 2-8 �C.
R: 42/43 S: 22-36/37
Chloramphenicol
5 g
25 g
100 g
C 1919
F
(Chloromycetin; D
NS3
protienase: structure and function of a zinc-
containing serine protease. Antivir. Ther., 3, 99-
109 (1998).
2. Xu, M.Q. and Evans, T.C. Jr. Intein-mediated
ligation and cyclization of expressed
α-Synuclein Neurodegeneration". University of
Pennsylvania, Ph.D. dissertation, pp. 42, 43, 48,
49, 77, 96, 97, 99 (2011).
9. Yu, Daozhan, "Omentin Activates AMP-activated
Protein Kinase and Plays
Reagent 1
PHYSICAL DESCRIPTION:
Appearance: White Powder
Melting point: 42-43EC
Molecular weight : 154.1
Purity: 99% by titration
Formula: C H O S4 10 2 2
METHOD OF PREPARATION:
DTT is synthetic
Immediately place the tube on ice for 10 minutes.
5. Heat shock the cells for 45-50 seconds in a 42°C
water bath (critical step). DO NOT SHAKE.
6. Immediately place the tubes on ice for
Anti-Actin, -Smooth Muscle specifically recognizes
the smooth muscle actin isoform of actin (42 kDa) in
ELISA and immunoblotting.1 It does not react with the
other major actin isoforms present