A6419

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B cells from Patients with Rheumatoid Arthritis Show Conserved CD39-Mediated Regulatory Function and increased CD39 Expression After Positive Response to Therapy.

E R Zacca et al.

Journal of molecular biology, 433(1), 166687-166687 (2020-10-26)

Rheumatoid arthritis (RA) is a chronic inflammatory disease characterized by progressive joint destruction associated with increased pro-inflammatory mediators. In inflammatory microenvironments, exogenous ATP (eATP) is hydrolyzed to adenosine, which exerts immunosuppressive effects, by the consecutive action of the ectonucleotidases CD39

PTEN Suppresses Glycolysis by Dephosphorylating and Inhibiting Autophosphorylated PGK1.

Xu Qian et al.

Molecular cell, 76(3), 516-527 (2019-09-08)

The PTEN tumor suppressor is frequently mutated or deleted in cancer and regulates glucose metabolism through the PI3K-AKT pathway. However, whether PTEN directly regulates glycolysis in tumor cells is unclear. We demonstrate here that PTEN directly interacts with phosphoglycerate kinase

Anthocyanins suppress the secretion of proinflammatory mediators and oxidative stress, and restore ion pump activities in demyelination.

Fabiano B Carvalho et al.

The Journal of nutritional biochemistry, 26(4), 378-390 (2015-01-31)

The aim of this study was to investigate the protective effect of anthocyanins (ANT) on oxidative and inflammatory parameters, as well as ion pump activities, in the pons of rats experimentally demyelinated with ethidium bromide (EB). Rats were divided in

Infection by Toxoplasma gondii, a severe parasite in neonates and AIDS patients, causes impaired anion secretion in airway epithelia.

Hong-Mei Guo et al.

Proceedings of the National Academy of Sciences of the United States of America, 112(14), 4435-4440 (2015-04-02)

The airway epithelia initiate and modulate the inflammatory responses to various pathogens. The cystic fibrosis transmembrane conductance regulator-mediated Cl(-) secretion system plays a key role in mucociliary clearance of inhaled pathogens. We have explored the effects of Toxoplasma gondii, an

A self-referencing biosensor for real-time monitoring of physiological ATP transport in plant systems.

Diana C Vanegas et al.

Biosensors & bioelectronics, 74, 37-44 (2015-06-22)

The objective of this study was to develop a self-referencing electrochemical biosensor for the direct measurement of ATP flux into the extracellular matrix by living cells/organisms. The working mechanism of the developed biosensor is based on the activity of glycerol

Vitamin C kills thyroid cancer cells through ROS-dependent inhibition of MAPK/ERK and PI3K/AKT pathways via distinct mechanisms.

Xi Su et al.

Theranostics, 9(15), 4461-4473 (2019-07-10)

Background: Vitamin C has been demonstrated to kill BRAF mutant colorectal cancer cells selectively. BRAF mutation is the most common genetic alteration in thyroid tumor development and progression; however, the antitumor efficacy of vitamin C in thyroid cancer remains to

Protocol for High-Throughput Screening of Neural Cell or Brain Tissue Protein Using a Dot-Blot Technique with Near-Infrared Imaging.

Anna C Chlebowski et al.

STAR protocols, 1(2) (2020-10-13)

Dot blotting allows for the rapid screening of a larger number of samples and/or targets than more traditional methods, such as a western blot or in-tissue-based methods. We have developed a dot-blot assay specifically for use with a LiCor Odyssey

Zach Geisterfer et al.

Bio-protocol, 7(7), e2217-e2217 (2017-04-05)

Kinesins play a role in organizing the mitotic spindle through the crosslinking of microtubules (MTs), made possible through binding sites at opposite ends of the holoenzyme. Here, we developed a method to test kinesin MT crosslinking action under physiological conditions.

Statistical models discriminating between complex samples measured with microfluidic receptor-cell arrays.

Ron Wehrens et al.

PloS one, 14(4), e0214878-e0214878 (2019-04-09)

Data analysis for flow-based in-vitro receptomics array, like a tongue-on-a-chip, is complicated by the relatively large variability within and between arrays, transfected DNA types, spots, and cells within spots. Simply averaging responses of spots of the same type would lead

The novel protein kinase C epsilon isoform modulates acetylcholine release in the rat neuromuscular junction.

Teresa Obis et al.

Molecular brain, 8(1), 80-80 (2015-12-03)

Various protein kinase C (PKC) isoforms contribute to the phosphorylating activity that modulates neurotransmitter release. In previous studies we showed that nPKCε is confined in the presynaptic site of the neuromuscular junction and its presynaptic function is activity-dependent. Furthermore, nPKCε

Patch-Clamp Analysis of the Mitochondrial H+ Leak in Brown and Beige Fat.

Ambre M Bertholet et al.

Frontiers in physiology, 11, 326-326 (2020-05-01)

Mitochondria convert the chemical energy of metabolic substrates into adenosine triphosphate (ATP) and heat. Although ATP production has become a focal point of research in bioenergetics, mitochondrial thermogenesis is also crucial for energy metabolism. Mitochondria generate heat due to H+

Wedelolactone facilitates Ser/Thr phosphorylation of NLRP3 dependent on PKA signalling to block inflammasome activation and pyroptosis.

Hao Pan et al.

Cell proliferation, 53(9), e12868-e12868 (2020-07-14)

Wedelolactone exhibits regulatory effects on some inflammatory diseases. However, the anti-inflammatory mechanism of wedelolactone has not been entirely unravelled. Therefore, the present study focuses on investigating the mechanism of wedelolactone on NLRP3 inflammasome in macrophages and its influence on MSU-induced

Tim-4 Inhibits NLRP3 Inflammasome via the LKB1/AMPKα Pathway in Macrophages.

Wen Liu et al.

Journal of immunology (Baltimore, Md. : 1950), 203(4), 990-1000 (2019-07-03)

Nonalcoholic fatty liver disease (NAFLD), characterized by excessive inflammation and lipid deposition, is one of the most common metabolic liver diseases. The expression of NLRP3 inflammasome in macrophages is significantly increased in NAFLD, and its activation aggravates NAFLD greatly. Tim-4

Facile enzymatic synthesis of sugar 1-phosphates as substrates for phosphorylases using anomeric kinases.

Yuan Liu et al.

Carbohydrate research, 401, 1-4 (2014-12-03)

Three sugar 1-phosphates that are donor substrates for phosphorylases were produced at the gram scale from phosphoenolpyruvic acid and the corresponding sugars by the combined action of pyruvate kinase and the corresponding anomeric kinases in good yields. These sugar 1-phosphates

PhospholipaseCγ1/calcium-dependent membranous localization of Gsdmd-N drives endothelial pyroptosis, contributing to lipopolysaccharide-induced fatal outcome.

Hong Liu et al.

American journal of physiology. Heart and circulatory physiology, 319(6), H1482-H1495 (2020-10-17)

Multiple organ perfusion is impaired in sepsis. Clinical studies suggest that persistent perfusion disturbances are prognostic of fatal outcome in sepsis. Pyroptosis occurs upon activation of caspases and their subsequent cleavage of gasdermin D (Gsdmd), resulting in Gsdmd-N (activated NH2-terminal

Zn(II)-Coordinated Quantum Dot-FRET Nanosensors for the Detection of Protein Kinase Activity.

Butaek Lim et al.

Sensors (Basel, Switzerland), 15(8), 17977-17989 (2015-07-28)

We report a simple detection of protein kinase activity using Zn(II)-mediated fluorescent resonance energy transfer (FRET) between quantum dots (QDs) and dye-tethered peptides. With neither complex chemical ligands nor surface modification of QDs, Zn(II) was the only metal ion that

Deuterated nucleotides as chemical probes of RNA structure: a detailed protocol for the enzymatic synthesis of a complete set of nucleotides specifically deuterated at ribose carbons

Tullius T, et al.

ScienceOpen Research (2018)

Effect of interleukin-31 on septic shock through regulating inflammasomes and interleukin-1beta

Gu X, et al.

Experimental and Therapeutic Medicine, 16(1), 171-177 (2018)

Integrated use of LC/MS/MS and LC/Q-TOF/MS targeted metabolomics with automated label-free microscopy for quantification of purine metabolites in cultured mammalian cells.

S Eric Nybo et al.

Purinergic signalling, 15(1), 17-25 (2019-01-04)

Purine metabolites have been implicated as clinically relevant biomarkers of worsening or improving Parkinson's disease (PD) progression. However, the identification of purine molecules as biomarkers in PD has largely been determined using non-targeted metabolomics analysis. The primary goal of this

Screening of novel Midkine binding protein by BioID2-based proximity labeling.

Yosuke Komata et al.

Nagoya journal of medical science, 83(3), 495-508 (2021-09-24)

Midkine (MK), a heparin-binding growth factor, is associated with the poor prognosis of the pediatric tumor, neuroblastoma. MK would be a druggable target as many studies showed inhibition of its function in various cancers suppressed tumor developments. To establish the

Protective Effect of α-Linolenic Acid on Non-Alcoholic Hepatic Steatosis and Interleukin-6 and -10 in Wistar Rats.

Camila Jordão Candido et al.

Nutrients, 12(1) (2019-12-22)

Consumption of omega-3 (n-3) polyunsaturated fatty acids (PUFA) is related to improvement in the inflammatory response associated with decreases in metabolic disorders of obesity, such as low-grade inflammation and hepatic steatosis. Linseed (Linum usitatissimum) oil is a primary source of

Microglia modulate hippocampal synaptic transmission and sleep duration along the light/dark cycle.

Giorgio Corsi et al.

Glia, 70(1), 89-105 (2021-09-07)

Microglia, the brain's resident macrophages, actively contribute to the homeostasis of cerebral parenchyma by sensing neuronal activity and supporting synaptic remodeling and plasticity. While several studies demonstrated different roles for astrocytes in sleep, the contribution of microglia in the regulation

Relaxin Can Mediate Its Anti-Fibrotic Effects by Targeting the Myofibroblast NLRP3 Inflammasome at the Level of Caspase-1.

Anita A Pinar et al.

Frontiers in pharmacology, 11, 1201-1201 (2020-08-28)

The NLRP3 inflammasome produces interleukin (IL)-1β and IL-18, which when chronically activated by transforming growth factor (TGF)-β1, contribute to fibrosis. The recombinant form of the anti-fibrotic hormone, relaxin (RLX), suppresses the pro-fibrotic influence of TGF-β1 and toll-like receptor (TLR)-4 on

Cutting Edge: G Protein Subunit β 1 Negatively Regulates NLRP3 Inflammasome Activation.

Tomohiko Murakami et al.

Journal of immunology (Baltimore, Md. : 1950), 202(7), 1942-1947 (2019-02-20)

The NLRP3 inflammasome has important roles in the pathogenesis of various inflammatory diseases. However, the regulatory mechanisms of the NLRP3 inflammasome are not fully understood. In this study, we attempted to identify molecules that interact with NLRP3 upon its activation.

Crosstalk between purinergic receptors and canonical signaling pathways in the mouse salivary gland.

Sumit Bhattacharya et al.

Cell calcium, 58(6), 589-597 (2015-10-08)

Isolated clusters of mouse parotid acinar cells in combination with live cell imaging were used to explore the crosstalk in molecular signaling between purinergic, cholinergic and adrenergic pathways that integrate to control fluid and protein secretion. This crosstalk was manifested

Atomic Structure of the Francisella T6SS Central Spike Reveals a Unique α-Helical Lid and a Putative Cargo.

Xue Yang et al.

Structure (London, England : 1993), 27(12), 1811-1819 (2019-11-05)

Francisella bacteria rely on a phylogenetically distinct type VI secretion system (T6SS) to escape host phagosomes and cause the fatal disease tularemia, but the structural and molecular mechanisms involved are unknown. Here we report the atomic structure of the Francisella

Inhibition of NLRP3 Inflammasome Activation and Pyroptosis in Macrophages by Taraxasterol Is Associated With Its Regulation on mTOR Signaling.

Fan Yang et al.

Frontiers in immunology, 12, 632606-632606 (2021-03-09)

Taraxasterol (TAS) is an active ingredient of Dandelion (Taraxacum mongolicum Hand. -Mazz.), a medicinal plant that has long been used in China for treatment of inflammatory disorders. But the underlying mechanism for its therapeutic effects on inflammatory disorders is not

Potential value of adenosine 5?-triphosphate (ATP) and adenosine in anaesthesia and intensive care medicine

Skrabanja ATP, et al.

British Journal of Anaesthesia, 94(5), 556-562 (2005)

Sensory Discrimination of Blood and Floral Nectar by Aedes aegypti Mosquitoes.

Veronica Jové et al.

Neuron, 108(6), 1163-1180 (2020-10-14)

Blood-feeding mosquitoes survive by feeding on nectar for metabolic energy but require a blood meal to develop eggs. Aedes aegypti females must accurately discriminate blood and nectar because each meal promotes mutually exclusive feeding programs with distinct sensory appendages, meal

Adenosine and its receptors as therapeutic targets: an overview

Sachdeva S and Gupta M

Saudi Pharmaceutical Journal : SPJ : The Official Publication of the Saudi Pharmaceutical Society, 21(3), 245-253 (2013)

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