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L G Carr et al.
Alcoholism, clinical and experimental research, 13(4), 594-596 (1989-08-01)
All nine exons of the ADH2(3) allele, which encodes the human alcohol dehydrogenase beta 3 subunit, have been cloned and sequenced. Comparison of this sequence to the ADH2(1) nucleotide sequence revealed only a single difference that results in an amino
T D Hurley et al.
Journal of molecular biology, 239(3), 415-429 (1994-06-10)
The three-dimensional structures of three variants of human beta alcohol dehydrogenase have been determined to 2.5 A resolution. These three structures differ only in the amino acid at position 47 and the molecules occupying the alcohol binding site. Human beta
Atsushi Yahashiri et al.
Biochemistry, 53(5), 881-894 (2014-01-21)
A role for protein dynamics in enzymatic catalysis of hydrogen transfer has received substantial scientific support, but the connections between protein structure and catalysis remain to be established. Valine residues 203 and 207 are at the binding site for the
M Filopanti et al.
European journal of endocrinology, 170(2), 247-254 (2013-11-13)
Hepatotoxicity is one of the most serious adverse effects in acromegalic patients treated with pegvisomant (PEG-V). Recent studies have found an association between this adverse event and the UGT1A1 allele 28 polymorphism associated with Gilbert's syndrome. To determine whether UGT1A1*28
Begüm Akduman et al.
Materials science & engineering. C, Materials for biological applications, 33(8), 4842-4848 (2013-10-08)
In this study, poly(2-hydroxyethyl methacrylate-glycidylmethacrylate) [poly(HEMA-GMA)] cryogels were prepared by radical cryocopolymerization of HEMA with GMA as a functional comonomer and N,N'-methylene-bisacrylamide (MBAAm) as a crosslinker. Iminodiacetic acid (IDA) functional groups were attached via ring opening of the epoxy group
Jonathan P Williams et al.
Analytical chemistry, 92(5), 3674-3681 (2020-01-31)
Electron-based fragmentation methods have revolutionized biomolecular mass spectrometry, in particular native and top-down protein analysis. Here, we report the use of a new electromagnetostatic cell to perform electron capture dissociation (ECD) within a quadrupole/ion mobility/time-of-flight mass spectrometer. This cell was
Y Matsuo et al.
European journal of biochemistry, 183(2), 317-320 (1989-08-01)
Allelic differences at the alcohol dehydrogenase (ADH) and aldehyde dehydrogenase loci may have an important role in an individual's alcohol sensitivity. We have cloned and sequenced all nine exons of an ADH2(2) allele which codes for an 'atypical' ADH, ADH
Juliane Zimmerling et al.
Applied biochemistry and biotechnology, 182(3), 1095-1107 (2017-01-08)
Herein, different dehydrogenases (DH) were characterized by applying a novel two-step enzyme assay. We focused on the NAD(P)
M W Hur et al.
Gene, 121(2), 305-311 (1992-11-16)
Human chi-alcohol dehydrogenase (chi-ADH) is a zinc-containing dimeric enzyme responsible for the oxidation of long-chain alcohols and omega-hydroxyfatty acids. Class-III ADHs, of which chi-ADH is the prototype, are widely produced and well conserved during evolution. This suggests that they fulfill
Xiaojuan Ma et al.
eLife, 9 (2020-04-07)
Tau hyper-phosphorylation and deposition into neurofibrillary tangles have been found in brains of patients with Alzheimer's disease (AD) and other tauopathies. Molecular chaperones are involved in regulating the pathological aggregation of phosphorylated Tau (pTau) and modulating disease progression. Here, we
Savarimuthu Baskar Raj et al.
Biochemistry, 53(36), 5791-5803 (2014-08-27)
Yeast (Saccharomyces cerevisiae) alcohol dehydrogenase I (ADH1) is the constitutive enzyme that reduces acetaldehyde to ethanol during the fermentation of glucose. ADH1 is a homotetramer of subunits with 347 amino acid residues. A structure for ADH1 was determined by X-ray
Jared Houghtaling et al.
ACS nano, 13(5), 5231-5242 (2019-04-18)
This paper demonstrates that high-bandwidth current recordings in combination with low-noise silicon nitride nanopores make it possible to determine the molecular volume, approximate shape, and dipole moment of single native proteins in solution without the need for labeling, tethering, or
Min-Kyu Kwak et al.
FEBS letters, 588(7), 1144-1153 (2014-03-13)
We purified a fraction that showed NAD(+)-linked methylglyoxal dehydrogenase activity, directly catalyzing methylglyoxal oxidation to pyruvate, which was significantly increased in glutathione-depleted Candida albicans. It also showed NADH-linked methylglyoxal-reducing activity. The fraction was identified as a NAD(+)-linked alcohol dehydrogenase (ADH1)
Kazuya Wada et al.
Rapid communications in mass spectrometry : RCM, 25(7), 960-968 (2011-03-19)
We developed a novel software named i-RUBY (identification-Related qUantification-Based strategY algorithm for liquid chromatography/tandem mass spectrometry (LC/MS/MS) data) that enables us to perform fully automatic ion current-based spectral feature analysis of highly accurate data obtained by LC/MS/MS. At the 1st
Shay Vimer et al.
Nature protocols, 15(2), 236-265 (2020-01-17)
Proteins derived by recombinant technologies must be characterized to ensure quality, consistency and optimal production. These properties are usually assayed following purification procedures that are time consuming and labor intensive. Here, we describe a native mass spectrometry (MS) approach, direct-MS
T P Pirog et al.
Mikrobiolohichnyi zhurnal (Kiev, Ukraine : 1993), 75(5), 18-26 (2014-02-01)
The effect of yeast autolysate and microelements on synthesis of surface-active substances (SAS, biosurfactants) was investigated under cultivation of Acinetobacter calcoaceticus IMV B-7241 on various carbon substrates (n-hexadecane, ethanol, glycerol). The authors have shown a possibility to substitute the yeast
Y Matsuo et al.
FEBS letters, 243(1), 57-60 (1989-01-16)
The structure and nucleotide sequence of an allele at the ADH1 locus have been determined. The nucleotide sequence of this allele is identical to that of a cDNA clone [(1986) Biochemistry 25, 2465-2470] and the intron positions of the ADH1
Xingxing Diao et al.
Drug metabolism and disposition: the biological fate of chemicals, 41(2), 430-444 (2012-11-22)
3-n-Butylphthalide (NBP) is a cardiovascular drug currently used for the treatment of cerebral ischemia. The present study aims to investigate the metabolism, pharmacokinetics, and excretion of NBP in humans and identify the enzymes responsible for the formation of major metabolites.
H von Bahr-Lindström et al.
Gene, 103(2), 269-274 (1991-07-22)
Human alcohol dehydrogenase (ADH) constitutes a set of isozymes and enzymes with different tissue and substrate specificities. The subunits are coded for by at least five gene loci, ADH1-ADH5. We now report the cloning and analysis of the human ADH4
Dongmei Yin et al.
Plant science : an international journal of experimental plant biology, 212, 48-54 (2013-10-08)
A PCR strategy was used to isolate a full-length CgADH (alcohol dehydrogenase) cDNA from chrysanthemum. The gene putatively encodes a 378 residue polypeptides, which shares 95% homology with tomato alcohol dehydrogenase class III. Endogenous ethylene generated in waterlogged Chrysanthemum zawadskii
K Engeland et al.
Proceedings of the National Academy of Sciences of the United States of America, 90(6), 2491-2494 (1993-03-15)
The origin of the fatty acid activation and formaldehyde dehydrogenase activity that distinguishes human class III alcohol dehydrogenase (alcohol:NAD+ oxidoreductase, EC 1.1.1.1) from all other alcohol dehydrogenases has been examined by site-directed mutagenesis of its Arg-115 residue. The Ala- and
Mark D Redwood et al.
FEMS microbiology letters, 278(1), 48-55 (2007-11-13)
Escherichia coli can perform at least two modes of anaerobic hydrogen metabolism and expresses at least two types of hydrogenase activity. Respiratory hydrogen oxidation is catalysed by two 'uptake' hydrogenase isoenzymes, hydrogenase -1 and -2 (Hyd-1 and -2), and fermentative
J O Höög et al.
European journal of biochemistry, 159(2), 215-218 (1986-09-01)
cDNA clones corresponding to two alleles of the ADH3 locus were identified by hybridization with synthetic oligodeoxyribonucleotides specific for class I human liver alcohol dehydrogenase. Sequences were determined for a 1457-nucleotide cDNA, covering the whole gamma 2-coding region, and a
Kate M Ehrensberger et al.
The Journal of biological chemistry, 288(2), 759-769 (2012-12-12)
In yeast, Adh1 (alcohol dehydrogenase 1) is an abundant zinc-binding protein that is required for the conversion of acetaldehyde to ethanol. Through transcriptome profiling of the Schizosaccharomyces pombe genome, we identified a natural antisense transcript at the adh1 locus that
Shuo Zhou et al.
Biotechnology letters, 35(3), 359-365 (2012-11-20)
The gene encoding a novel short-chain alcohol dehydrogenase in the thermophilic bacterium, Carboxydothermus hydrogenoformans, was identified and overexpressed in Escherichia coli. The enzyme was thermally stable and displayed the highest activity at 70 °C and pH 6.0. It preferred NAD(H) over
Christophe A Marquette et al.
Analytical and bioanalytical chemistry, 390(1), 155-168 (2007-10-03)
The present review draws a general picture of the bioanalytical applications of electro-chemiluminescent reactions (ECL). Only the two main ECL reactions-i.e. the luminol-based and Ru(bpy)(3)(2+)-based reactions-are considered for application in the fields of enzyme biosensors, immunochemical biosensors, DNA biosensors, and
J Hempel et al.
European journal of biochemistry, 145(3), 437-445 (1984-12-17)
Determination of the amino acid sequence of the beta 1 subunit from the class I (pyrazole-sensitive) human liver alcohol dehydrogenase isoenzyme beta 1 beta 1 revealed a 373-residue structure differing at 48 positions (including a gap) from that of the
Karolina Orywal et al.
Anticancer research, 33(9), 3725-3730 (2013-09-12)
The aim of this study was to investigate the potential role of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) as tumor markers for endometrial cancer. Serum samples were obtained from 40 women with endometrial cancer, 52 with myoma uteri and
Kenta Iitani et al.
The Analyst, 145(8), 2915-2924 (2020-03-07)
Skin gas that contains volatile metabolites (volatilome) is emanated continuously and is thus expected to be suitable for non-invasive monitoring. The aim of this study was to investigate the relationship between the regional difference of sweat rate and skin volatilome
Effects and action mechanisms of Korean pear (Pyrus pyrifolia cv. Shingo) on alcohol detoxification.
Ho-Sun Lee et al.
Phytotherapy research : PTR, 26(11), 1753-1758 (2012-03-28)
Korean pear (Pyrus pyrifolia cv. Shingo) has been used as a traditional medicine for alleviating alcohol hangover. However, scientific evidence for its effectiveness or mechanism is not clearly established. To investigate its mechanism of alcohol detoxification, both in vitro and
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