B3770

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A new biotinylating system for DNA using biotin aminocaproyl hydrazide and glutaraldehyde.

T Takahashi et al.

Nucleic acids research, 17(12), 4899-4900 (1989-06-26)

Quantitation of glycoproteins on electroblots using the biotin-streptavidin complex.

D J O'Shannessy et al.

Analytical biochemistry, 163(1), 204-209 (1987-05-15)

A method for the detection and quantitation of glycoproteins on nitrocellulose electroblots is described. Protein mixtures may be solubilized in sodium dodecyl sulfate prior to labeling, which is especially useful when dealing with membrane proteins. Mild periodate oxidation produces aldehydes

SdsA1, a secreted sulfatase, contributes to the in vivo virulence of Pseudomonas aeruginosa in mice.

Yutaka Kida et al.

Microbiology and immunology, 64(4), 280-295 (2020-01-08)

Mucin is a glycoprotein that is the primary component of the mucus overlaying the epithelial tissues. Because mucin functions as a first line of the innate immune system, Pseudomonas aeruginosa appears to require interaction with mucin to establish infection in

Computational methods for predicting 3D genomic organization from high-resolution chromosome conformation capture data.

Kimberly MacKay et al.

Briefings in functional genomics, 19(4), 292-308 (2020-05-01)

The advent of high-resolution chromosome conformation capture assays (such as 5C, Hi-C and Pore-C) has allowed for unprecedented sequence-level investigations into the structure-function relationship of the genome. In order to comprehensively understand this relationship, computational tools are required that utilize

Identification of an Atg8-Atg3 protein-protein interaction inhibitor from the medicines for Malaria Venture Malaria Box active in blood and liver stage Plasmodium falciparum parasites.

Adelaide U P Hain et al.

Journal of medicinal chemistry, 57(11), 4521-4531 (2014-05-03)

Atg8 is a ubiquitin-like autophagy protein in eukaryotes that is covalently attached (lipidated) to the elongating autophagosomal membrane. Autophagy is increasingly appreciated as a target in diverse diseases from cancer to eukaryotic parasitic infections. Some of the autophagy machinery is

Receptor binding activities of biotinylated derivatives of beta-nerve growth factor.

M B Rosenberg et al.

Journal of neurochemistry, 46(2), 641-648 (1986-02-01)

beta-nerve growth factor (NGF) was modified by biotinylation via carboxyl group substitution (C-bio-NGF) using biotin hydrazide and the coupling reagent 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide, under reaction conditions that yielded an average of 3 biotin additions per NGF subunit. NGF was also biotinylated through

Chondroitin sulfate E blocks enzymatic action of heparanase and heparanase-induced cellular responses.

Nobuaki Higashi et al.

Biochemical and biophysical research communications, 520(1), 152-158 (2019-10-05)

We examined whether chondroitin sulfates (CSs) exert inhibitory effects on heparanase (Hpse), the sole endoglycosidase that cleaves heparan sulfate (HS) and heparin, which also stimulates chemokine production. Hpse-mediated degradation of HS was suppressed in the presence of glycosaminoglycans derived from

Membrane sialoglycolipids emerging as possible signal transducers for lymphocyte stimulation.

S Spiegel et al.

Journal of immunology (Baltimore, Md. : 1950), 127(2), 572-575 (1981-08-01)

Biotin hydrazide was attached covalently to the aldehyde groups produced by periodate oxidation of bovine brain gangliosides. These modified gangliosides were incorporated into mature rat thymocytes by incubation of the biotinyl gangliosides in the culture medium containing these cells. Avidin

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