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关键词:'B9879'
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Robert E Kingston et al.
Current protocols in molecular biology, Chapter 9, Unit 9-Unit 9 (2008-02-12)
This unit presents two methods of calcium phosphate-based eukaryotic cell transfection that can be used for both transient and stable transfections. In these protocols, plasmid DNA is introduced to monolayer cell cultures via a precipitate that adheres to the cell
Frank Li et al.
Human molecular genetics, 24(18), 5219-5233 (2015-07-01)
Nebulin is a giant filamentous protein that is coextensive with the actin filaments of the skeletal muscle sarcomere. Nebulin mutations are the main cause of nemaline myopathy (NEM), with typical adult patients having low expression of nebulin, yet the roles
T K Kim et al.
Antonie van Leeuwenhoek, 45(4), 581-593 (1979-01-01)
The effect of various factors such as sodium chloride, sodium citrate, pH, linase by Staphylococcus aureus ATCC 14458 was investigated. Penicillinase was measured at selected time intervals from supernates of cultures grown in Antibiotic Medium 3 broth containing various concentrations
Junki Mine et al.
The Journal of general virology, 96(Pt 7), 1746-1756 (2015-03-27)
The viral protein Npro is unique to the genus Pestivirus within the family Flaviviridae. After autocatalytic cleavage from the nascent polyprotein, Npro suppresses type I IFN (IFN-α/β) induction by mediating proteasomal degradation of IFN regulatory factor 3 (IRF-3). Previous studies
Yonggang Wang et al.
Journal of the American Chemical Society, 137(34), 11144-11149 (2015-08-19)
Hydrostatic pressure, as an alternative of chemical pressure to tune the crystal structure and physical properties, is a significant technique for novel function material design and fundamental research. In this article, we report the phase stability and visible light response
E Csuhai et al.
Archives of biochemistry and biophysics, 362(2), 291-300 (1999-02-17)
N-arginine dibasic convertase cleaves polypeptides between paired basic residues containing the sequence Arg-Arg or Arg-Lys. The enzyme contains a large anionic domain, which in the rat enzyme consists of 57 acidic residues out of a stretch of 76 amino acids.
Ting Liu et al.
Biotechnology and bioengineering, 112(10), 2051-2059 (2015-04-23)
Electroactive biofilms play essential roles in determining the power output of microbial fuel cells (MFCs). To engineer the electroactive biofilm formation of Shewanella oneidensis MR-1, a model exoelectrogen, we herein heterologously overexpressed a c-di-GMP biosynthesis gene ydeH in S. oneidensis
Brian A Karolewski et al.
Human gene therapy, 14(14), 1287-1296 (2003-09-25)
A number of different transfection reagents have been used for lentiviral vector production. We directly compared transfection buffers, DNA purification methods, chemical facilitators, and DNA concentrations to optimize production. The use of N,N-bis (2-hydroxyethyl)-2-aminoethanesulfonic acid (BES), sodium butyrate, and one
Riley E Brandt et al.
The journal of physical chemistry letters, 6(21), 4297-4302 (2015-11-06)
Guided by predictive discovery framework, we investigate bismuth triiodide (BiI3) as a candidate thin-film photovoltaic (PV) absorber. BiI3 was chosen for its optical properties and the potential for "defect-tolerant" charge transport properties, which we test experimentally by measuring optical absorption
Marcus Maschke et al.
Chembiochem : a European journal of chemical biology, 16(9), 1333-1342 (2015-05-28)
We report the synthesis of metallocene compounds Cp2 M with two different electron-withdrawing substituents on both cyclopentadienyl rings (hexafluoroacetone (HFA) and chlorobenzoyl (1-5); HFA and COOH (6 and 7), M=Fe or Ru). The COOH-containing derivatives were used to synthesize peptide
Nihar R Pradhan et al.
ACS applied materials & interfaces, 7(22), 12080-12088 (2015-05-20)
Here, we report the photoconducting response of field-effect transistors based on three atomic layers of chemical vapor transport grown WSe2 crystals mechanically exfoliated onto SiO2. We find that trilayered WSe2 field-effect transistors, built with the simplest possible architecture, can display
Yingjie Zhang et al.
Nano letters, 15(7), 4657-4663 (2015-06-24)
Noncrystalline semiconductor materials often exhibit hysteresis in charge transport measurements whose mechanism is largely unknown. Here we study the dynamics of charge injection and transport in PbS quantum dot (QD) monolayers in a field effect transistor (FET). Using Kelvin probe
John A Ormiston et al.
EuroIntervention : journal of EuroPCR in collaboration with the Working Group on Interventional Cardiology of the European Society of Cardiology, 10(12), 1449-1455 (2014-11-26)
Stent fracture is important because it may cause adverse events. The interventional cardiologist needs independent data to aid stent selection. Stent designers need data to improve stent design. We used a repetitive bend test to compare durability and fracture for
R N Fabricant et al.
Proceedings of the National Academy of Sciences of the United States of America, 74(2), 565-569 (1977-02-01)
Purified mouse nerve growth factor (NGF)radiolabeled with 125I was tested for its ability to bind to a variety of different cultured cells. NGF binds readily to human and hamster melanoma cells but does not bind to many other cell lines.
Mara Fernandes Maranhão et al.
BMC psychiatry, 15, 194-194 (2015-08-13)
Binge eating disorder is a new category in DSM-5 and highly associated with higher body mass index. The neural mechanisms that underlie binge eating are of great interest in order to improve treatment interventions. Brain mechanisms underlying drug and food
D N Barry
The Australian journal of experimental biology and medical science, 60(Pt 2), 167-174 (1982-04-01)
The effect on the haemoprotozoan parasite Babesia rodhaini of adding a zwitterion buffer, HEPES, to a basal medium was studied by comparing the glucose uptake, ATP concentration, medium pH and infectivity of parasitized rat erythrocytes every 4 h during 24
Kensuke Nishimiya et al.
Circulation journal : official journal of the Japanese Circulation Society, 79(6), 1323-1331 (2015-04-07)
Coronary adventitia harbors a wide variety of components, such as inflammatory cells and vasa vasorum (VV). Adventitial VV initiates the development of coronary artery diseases as an outside-in supply route of inflammation. We have recently demonstrated that drug-eluting stent implantation
Takashi Koyama et al.
Marine biotechnology (New York, N.Y.), 17(4), 502-510 (2015-05-16)
Unlike the conservation of sex-determining (SD) modes seen in most mammals and birds, teleost fishes exhibit a wide variety of SD systems and genes. Hence, the study of SD genes and sex chromosome turnover in fish is one of the
C A Poole et al.
In vitro, 18(9), 755-765 (1982-09-01)
Chick embryo epiphyseal chondrocytes cultured in media containing HEPES, TES, and BES zwitterion buffers, used in combination or independently, consistently developed cytoplasmic vacuoles. This cytoplasmic vacuolation was resolved when the zwitterion buffered media was replaced by media containing bicarbonate:CO2 enriched
Russell A Jensen et al.
The journal of physical chemistry letters, 6(15), 2933-2937 (2015-08-13)
Colloidal CdSe quantum dot (QD) core ensembles were photodimmed and allowed to recover in the dark using ambient thermal energy at a range of temperatures. Nonlinear thermal recovery is well described by a stretched exponential function, and further analysis yields
Tomohiro Itoh et al.
Free radical research, 45(9), 1033-1039 (2011-06-21)
This study aimed to examine the roles of reactive oxygen species (ROS) in cisplatin treatment of human prostate cancer cells; hormone-sensitive LNCaP and hormone-refractory PC3 and DU145 cells. Intracellular levels of ROS and H(2)O(2) were measured and visualized using specific
Jun Kyeung Ko et al.
Interventional neuroradiology : journal of peritherapeutic neuroradiology, surgical procedures and related neurosciences, 21(4), 511-519 (2015-06-13)
The purpose of this study is to assess the technical feasibility and clinical efficacy of percutaneous transluminal angioplasty and stenting (PTAS) for symptomatic stenosis of the intracranial extradural (petrous and cavernous) internal carotid artery (ICA).Review of medical records identified 26
Christos V Bourantas et al.
International journal of cardiology, 186, 178-185 (2015-04-02)
Numerous studies have demonstrated an association between endothelial shear stress (ESS) and neointimal formation after stent implantation. However, the role of ESS on the composition of neointima and underlying plaque remains unclear. Patients recruited in the Comfortable AMI-IBIS 4 study
Ryo Nishio et al.
International heart journal, 56(4), 389-394 (2015-06-30)
The early phase arterial reaction after implantation of second-generation drug-eluting stents (2nd DES) and baremetal stents (BMS) in patients with ST-segment elevation myocardial infarction (STEMI) remains unclear.The MECHANISM pilot study is a multi-center prospective registry that enrolled 24 STEMI patients
Brent Kitchen et al.
ACS applied materials & interfaces, 7(24), 13208-13216 (2015-06-02)
The role of molecular orientation of a polar conjugated polymer in polymer-fullerene organic photovoltaic (OPV) cells is investigated. A planar heterojunction (PHJ) OPV cell composed of poly(3-hexylthiophene) (P3HT) and [6,6]-phenyl C61-butyric acid methyl ester (PCBM) is used as a model
M K Higgins et al.
Body image, 14, 39-46 (2015-04-14)
Impulsivity has been linked to bulimic symptomatology in a number of studies; however, few have examined this relationship among Black women. We investigated the correlations between impulsivity and bulimic symptoms, and tested impulsivity as a moderator of the body shame/bulimic
Oliver Welz et al.
The journal of physical chemistry. A, 119(28), 7116-7129 (2015-04-11)
Low-temperature propane oxidation was studied at P = 4 Torr and T = 530, 600, and 670 K by time-resolved multiplexed photoionization mass spectrometry (MPIMS), which probes the reactants, intermediates, and products with isomeric selectivity using tunable synchrotron vacuum UV
T R Holoman et al.
Applied and environmental microbiology, 64(9), 3359-3367 (1998-09-03)
Defined microbial communities were developed by combining selective enrichment with molecular monitoring of total community genes coding for 16S rRNAs (16S rDNAs) to identify potential polychlorinated biphenyl (PCB)-dechlorinating anaerobes that ortho dechlorinate 2,3, 5,6-tetrachlorobiphenyl. In enrichment cultures that contained a
R P Pulis et al.
American journal of physiology. Heart and circulatory physiology, 279(5), H2151-H2158 (2000-10-25)
We investigated the metabolic effects of buffering agents alpha-amino-4-imidazole-propionic acid (Histidine), N, N-bis(2-hydroxyethyl)glycine (bicine), N, N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid (BES) on anaerobic energy production (via glycolysis) and conservation of key regulatory enzyme activity, and phosphofructokinase (PFK) throughout prolonged hypothermic hypoxia in porcine
Heike Vogel et al.
Nucleosides, nucleotides & nucleic acids, 32(1), 17-27 (2013-01-31)
Rapid, template-directed ligation reactions between a phosphate-terminated oligonucleotide and an unphosphorylated reaction partner may be induced by cyanogen bromide (BrCN). Frequently, however, the reaction is low yielding, and even a large excess of the condensing agent can fail to induce
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