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C6788

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显示 1-30 #N/A 56 条结果 关于 "C6788" 范围 论文
P Zhou et al.
Fresenius' journal of analytical chemistry, 367(5), 422-425 (2001-03-03)
Dot-immunobinding assay of hepatitis B surface antigen (HBsAg) was performed by a conventionally dot-ELISA technique with 4-chloro-1-naphthol staining, and the quantitative results were measured by an indirect photoacoustic method. In this method, a 2-mW helium-neon laser was applied to provide
Xiaohui Zhou et al.
Infection and immunity, 77(11), 5059-5070 (2009-09-10)
The specific contribution of interleukin-17/interleukin-17 receptor (IL-17/IL-17R)-mediated responses in regulating host susceptibility against obligatory intracellular Chlamydia infection was investigated in C57BL/6 and C3H/HeN mice during Chlamydia muridarum respiratory infection. We demonstrated that Chlamydia stimulated IL-17/IL-17R-associated responses in both Chlamydia-resistant C57BL/6
D J Litman et al.
Clinical chemistry, 29(9), 1598-1603 (1983-09-01)
We describe an internally referenced immunochemical test-strip for use in the rapid detection of morphine. The method is based on the "enzyme-channelling" immunoassay technique, and a glucose oxidase-horseradish peroxidase enzyme pair is used to immunospecifically generate an insoluble, colored reaction
Jung-Mi Kang et al.
Molecular and biochemical parasitology, 201(1), 66-71 (2015-06-24)
Paramyosin of Clonorchis sinensis (CsPmy) is a myofibrillar protein localized in subtegumental muscle, tegument, and the muscle layer surrounding the intestine of the parasite. Previously, we have identified that CsPmy reacted with sera of human clonorchiasis and this protein had
Daniela Staubli et al.
Parasitology research, 99(6), 648-658 (2006-05-24)
Neospora caninum ranges among the major causes of infectious abortion in cattle worldwide. The present study was designed to improve the serodiagnostic tools by complementing a conventional ELISA with a highly sensitive and species-specific N. caninum immunoblot. To evaluate this
Elizabeth Valdivieso et al.
Parasites & vectors, 8, 399-399 (2015-07-29)
Serine proteinase inhibitors (serpins) finely regulate serine proteinase activity via a suicide substrate-like inhibitory mechanism. In parasitic nematodes, some serpins interact with host physiological processes; however, little is known about these essential molecules in Anisakis. This article reports the gene
Ewa Rozycka-Sokolowska et al.
Acta crystallographica. Section C, Crystal structure communications, 65(Pt 5), o207-o210 (2009-05-02)
Molecules of the title compound, C(10)H(7)ClO, (I), are connected by a single strong O-H...O hydrogen bond into a simple C(2) chain, which runs parallel to the c axis and is additionally stabilized by intermolecular pi-pi stacking interactions. The significance of
R Kobayashi et al.
Analytical biochemistry, 183(1), 9-12 (1989-11-15)
A method which allows the highly sensitive and simple immunodetection of antigen-antibody complexes on nitrocellulose papers has been developed. The method is a modification of the procedure known as the "Nadi reaction" (oxidative coupling of 1-naphthol and N,N'-dimethyl-p-phenylendiamine) for histochemical
C F Frey et al.
Parasitology research, 104(6), 1269-1277 (2009-01-09)
Trichinellosis is a zoonotic disease in humans caused by Trichinella spp. According to international regulations and guidelines, serological surveillance can be used to demonstrate the absence of Trichinella spp. in a defined domestic pig population. Most enzyme-linked immunosorbent assay (ELISA)
M M Leong et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 34(12), 1645-1650 (1986-12-01)
An anti-peroxidase-anti-biotin hybrid hybridoma rat cell line, capable of producing a bispecific monoclonal antibody, has been derived to explore its use in conjunction with a luminol immunodetection system. Luminescence was detected using x-ray film. The method was sufficiently sensitive and
D G Baskin et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 30(7), 710-712 (1982-07-01)
Ultrastructural localization of insulin in B cells of guinea pig pancreas was accomplished after osmium fixation with an immunoperoxidase procedure that utilized 4-chloro-1-naphthol (CN) in the substrate solution. The principal features of this protocol were: a) osmium tetroxide postfixation; b)
A Domingo et al.
Analytical biochemistry, 182(1), 176-181 (1989-10-01)
As described in this article, visualization and/or photography under uv light of 4-chloro-1-naphthol-developed, peroxidase-marked immunoblots allows an increase in sensitivity of more than 100 times over the apparent staining results observable under normal visible white light. This increase in sensitivity
J R Shainoff et al.
Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis, 1(4-5), 479-484 (1990-10-01)
A novel electrophoretic procedure enabling multiple, direct immunoprobing of electrophoregrams without depending on Western blotting is described, and applied to the identification of the derivatives formed in the early stages of clot stabilization. Multicolour immunostainings for positive identification of cross-linked
Nakane, P.K.
The Journal of Histochemistry and Cytochemistry, 6, 557-557 (1968)
J A deLeo et al.
The Anatomical record, 209(3), 417-422 (1984-07-01)
Multiple staining of the endocrine cells of the pancreatic islet was studied in tissue obtained from adult rats. After fixation in Bouin's fluid and processing for light microscopy, the unlabeled peroxidase-antiperoxidase (PAP) technique was performed. Successful staining procedures used variations
D W Ross et al.
Archives of pathology & laboratory medicine, 109(11), 1005-1006 (1985-11-01)
The discovery of hereditary deficiency of myeloperoxidase (MPO) in neutrophils and monocytes of affected individuals has been based on the absence of cytochemical staining in these peripheral blood cells. We report that an immunocytochemical method shows more sensitivity than either
Yong Hun Kim et al.
The Korean journal of parasitology, 46(1), 29-32 (2008-03-18)
The aim of this study is to determine the Toxocara seropositive rate among healthy people with eosinophilia. A total of 97 people residing in Seoul who were healthy and whose blood eosinophilia was over 10%, as shown by regular health
T M Work et al.
Veterinary pathology, 52(6), 1195-1201 (2014-12-03)
Cancers in humans and animals can be caused by viruses, but virus-induced tumors are considered to be poor sites for replication of intact virions (lytic replication). Fibropapillomatosis (FP) is a neoplastic disease associated with a herpesvirus, chelonid herpesvirus 5 (ChHV5)
Lidia Patricia Jaramillo-Quintero et al.
Bioscience, biotechnology, and biochemistry, 79(6), 896-906 (2015-02-24)
The toxicity of the curcin on cancer cells allows to consider this protein as the toxic component of an immunotoxin directed to Her2, which is associated with cancer. Reductive amination was proposed to conjugate curcin and an anti-Her2; the binding
Lamia L'Hocine et al.
Food chemistry, 194, 820-827 (2015-10-17)
A full factorial design was used to assess the single and interactive effects of three non-denaturing aqueous (phosphate, borate, and carbonate) buffers at various ionic strengths (I) on allergen extractability from and immunoglobulin E (IgE) immunoreactivity of peanut, almond, hazelnut
M Aihara et al.
Thrombosis and haemostasis, 55(2), 263-267 (1986-04-30)
A technique for the detection of von Willebrand factor multimers separated by discontinuous SDS agarose electrophoresis has been developed using non-radioactive compounds. The multimeric patterns were visualized by monospecific anti-human vWF:Ag followed by incubation with biotinylated antibody. After addition of
B Wróblewska et al.
Food & function, 9(3), 1500-1509 (2018-02-09)
The objective of this work was to identify the nutritional and physiological effects of commercial soy and whey protein preparations. Wistar rats were fed with soy (S), whey (W), or casein (C) preparations as the sole dietary protein source. The
O Costa et al.
Pathologie-biologie, 35(7), 1095-1098 (1987-09-01)
We have demonstrated that alpha-naphthol is a peroxidase substrate that produces a stable staining of immunoblots, which may be kept for further examination and retrospective comparison.
M L Poor et al.
Analytical biochemistry, 175(1), 191-195 (1988-11-15)
A method has been developed which allows the simultaneous immunodetection of more than one type of protein on the same nitrocellulose membrane. This procedure does not require special labeling of samples or elution of antibodies from the membrane as do
Ming Yuan et al.
Cardiology, 131(1), 1-12 (2015-04-01)
High levels of soluble CD40 ligand (sCD40L) in the circulation have been suggested as an important indicator of cardiovascular diseases such as atherosclerosis and acute coronary syndromes. In the present study, we explored the role of sCD40L in the formation
Khuzwayo C Jere et al.
PloS one, 9(9), e105167-e105167 (2014-10-01)
Rotavirus virus-like particles (RV-VLPs) are potential alternative non-live vaccine candidates due to their high immunogenicity. They mimic the natural conformation of native viral proteins but cannot replicate because they do not contain genomic material which makes them safe. To date
X Su et al.
Analytical biochemistry, 299(2), 241-246 (2001-12-04)
This paper describes the use of a cuvette-based surface plasmon resonance (SPR) instrument to measure biocatalyzed precipitation reactions. Enzyme-modified SPR sensor disk forms the base of a cuvette, in which the substrate solution is added with stirring. The determination of
Li Hou et al.
Biosensors & bioelectronics, 47, 149-156 (2013-04-10)
A new sandwich-type impedimetric immunosensor based on functionalized graphene oxide nanosheets with a high ratio of horseradish peroxidase (HRP) and detection antibody was developed for the detection of carcinoembryonic antigen (CEA) by coupling with enzymatic biocatalytic precipitation of 4-chloro-1-naphthol (4-CN)
Uracha Ruktanonchai et al.
Analytical biochemistry, 429(2), 142-147 (2012-07-20)
The use of microarray-based immunoassay is often limited by its sensitivity. To increase the sensitivities of such an immunoassay, liposome encapsulation was explored. Two different liposome formations and several preparation methods were examined to optimize encapsulation and signal-enhancing efficacy for
Precolostral serology in calves born from Neospora-seropositive mothers
Staubli D, et al.
Parasitology Research, 99(4), 398-404 (2006)
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