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关键词:'C6905'
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Andy Maloney et al.
PloS one, 6(6), e19522-e19522 (2011-06-16)
In this study, we report differences in the observed gliding speed of microtubules dependent on the choice of bovine casein used as a surface passivator. We observed differences in both speed and support of microtubules in each of the assays.
Dorota Dulko et al.
Food research international (Ottawa, Ont.), 145, 110413-110413 (2021-06-12)
The gastrointestinal hydrolysis of food proteins has been portrayed in scientific literature to predominantly depend on the activity and specificity of proteolytic enzymes. Human bile has not been considered to facilitate proteolysis in the small intestine, but rather to assist
Dóra Mosztbacher et al.
Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.], 21(2), 342-352 (2021-02-03)
Mesotrypsin is a low-abundance human trypsin isoform with a unique evolutionary mutation that conferred resistance to trypsin inhibitors and restricted substrate specificity. Mesotrypsin degrades the serine protease inhibitor Kazal type 1 (SPINK1) and thereby might increase risk for pancreatitis. Here
Hiroshi Nakagawa et al.
Biophysical journal, 120(23), 5408-5420 (2021-11-01)
β-casein undergoes a reversible endothermic self-association, forming protein micelles of limited size. In its functional state, a single β-casein monomer is unfolded, which creates a high structural flexibility, which is supposed to play a major role in preventing the precipitation
Fang Huang et al.
eLife, 10 (2021-11-26)
The positive transcription elongation factor b (P-TEFb) is a critical coactivator for transcription of most cellular and viral genes, including those of HIV. While P-TEFb is regulated by 7SK snRNA in proliferating cells, P-TEFb is absent due to diminished levels
Ivo A Telley et al.
Methods in molecular biology (Clifton, N.J.), 777, 127-145 (2011-07-21)
Several microtubule-associated proteins localize in living cells selectively to an extended region at the growing microtubule plus ends. Over the last years, these plus-end-tracking proteins, also called +TIPs, have attracted considerable interest because they are involved in a large variety
Hendrik Bruns et al.
Journal of synchrotron radiation, 30(Pt 4), 788-795 (2023-05-26)
A sample environment and manipulation tool is presented for single-particle X-ray experiments in an aqueous environment. The system is based on a single water droplet, positioned on a substrate that is structured by a hydrophobic and hydrophilic pattern to stabilize
Stefan Niekamp et al.
Proceedings of the National Academy of Sciences of the United States of America, 116(10), 4275-4284 (2019-02-17)
Light microscopy is a powerful tool for probing the conformations of molecular machines at the single-molecule level. Single-molecule Förster resonance energy transfer can measure intramolecular distance changes of single molecules in the range of 2 to 8 nm. However, current
Stefan Niekamp et al.
Nature methods, 17(4), 437-441 (2020-03-24)
Photobleaching limits extended imaging of fluorescent biological samples. We developed DNA-based 'FluoroCubes' that are similar in size to the green fluorescent protein, have single-point attachment to proteins, have a ~54-fold higher photobleaching lifetime and emit ~43-fold more photons than single
T M Ellis et al.
Diabetologia, 41(6), 731-735 (1998-07-14)
Elevated cellular immune responses against the cows' milk protein beta casein have been reported in individuals with Type I diabetes mellitus, a finding supportive of the concept that cows' milk consumption may be causative for the disease. We analysed cellular
Jeongjoon Choi et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(14), 8074-8082 (2020-03-27)
The heat-stable nucleoid structuring (H-NS, also referred to as histone-like nucleoid structuring) protein silences transcription of foreign genes in a variety of Gram-negative bacterial species. To take advantage of the products encoded in foreign genes, bacteria must overcome the silencing
V Artegoitia et al.
Journal of animal physiology and animal nutrition, 97(5), 919-932 (2012-08-18)
The objective was to evaluate the effect of body condition score (BCS) at 30 days before calving (-30 days) induced by a differential nutritional management, parity and week of lactation (WOL) on milk yield and composition, and milk casein and fatty acid
Isabel Pablos et al.
Cell reports, 37(4), 109892-109892 (2021-10-22)
The main viral protease (3CLpro) is indispensable for SARS-CoV-2 replication. We delineate the human protein substrate landscape of 3CLpro by TAILS substrate-targeted N-terminomics. We identify more than 100 substrates in human lung and kidney cells supported by analyses of SARS-CoV-2-infected
F Valentino et al.
Soft matter, 12(47), 9429-9435 (2016-11-11)
Pulling membrane nanotubes from liposomes presents a powerful method to gain access to membrane mechanics. Here we extend classical optical tweezers studies to infer membrane nanotube dynamics with high spatial and temporal resolution. We first validate our force measurement setup
Javier Martínez-Botas et al.
Methods in molecular biology (Clifton, N.J.), 2344, 119-135 (2021-06-12)
Peptide microarrays have been used to study protein-protein interaction, enzyme-substrate profiling, epitope mapping, vaccine development, and immuno-profiling. Unlike proteins, peptides are cheap to produce, and can be produced in a high-throughput manner, in a reliable and consistent procedure that reduces
Yuxi Deng et al.
Journal of agricultural and food chemistry, 66(16), 4219-4232 (2018-04-05)
Based on trypsin specificity (for lysines and arginines), trypsins from different sources are expected to hydrolyze a given protein to the same theoretical maximum degree of hydrolysis (DHmax,theo). This is in contrast with experiments. Using α-lactalbumin and β-casein, this study
Hannes Maib et al.
Current biology : CB, 32(13), 2821-2833 (2022-05-25)
Polarized trafficking is necessary for the development of eukaryotes and is regulated by a conserved molecular machinery. Late steps of cargo delivery are mediated by the exocyst complex, which integrates lipid and protein components to tether vesicles for plasma membrane
Presence of functional, autoreactive human milk-specific IgE in infants with cow's milk allergy.
Jarvinen KM., et al
Clinical and Experimental Allergy (2011)
Hao Wang et al.
Journal of chromatography. A, 1564, 69-75 (2018-06-17)
Analysis of phosphoproteins always faces the challenge of low stoichiometry, which demands highly selective and efficient enrichment in the initial sample preparation. Here we report our synthesis of the novel titanium (IV) ion immobilized adenosine triphosphate functionalized silica nanoparticles (Ti4+-ATP-NPs)
Andrew R Harris et al.
Nature communications, 11(1), 5973-5973 (2020-11-27)
The assembly of actin filaments into distinct cytoskeletal structures plays a critical role in cell physiology, but how proteins localize differentially to these structures within a shared cytoplasm remains unclear. Here, we show that the actin-binding domains of accessory proteins
Julia Grawenhoff et al.
Methods in molecular biology (Clifton, N.J.), 2431, 547-568 (2022-04-13)
Motor protein-driven transport of mRNAs on microtubules and their local translation underlie important neuronal functions such as development, growth cone steering, and synaptic plasticity. While there is abundant data on how membrane-bound cargoes such as vesicles, endosomes, or mitochondria are
C Fuerer et al.
Journal of dairy science, 103(2), 1193-1207 (2019-11-25)
Infant formulations are constantly evolving as novel protein ingredients are added to make them more closely mimic the protein profile of human milk; however, precise analytical methods for characterizing and quantifying the major milk proteins in such formulations are currently
Javier Martínez-Botas et al.
Methods in molecular biology (Clifton, N.J.), 2578, 219-236 (2022-09-25)
Peptide microarrays are a powerful tool to identify linear epitopes of food allergens in a high-throughput manner. The main advantages of the microarray-based immunoassay are as follows: the possibility to assay thousands of targets simultaneously, the requirement of a low
Simone Talens et al.
Thrombosis and haemostasis, 119(10), 1624-1631 (2019-08-23)
Many proteins bind to fibrin during clot formation in plasma. We previously identified by mass spectrometry the most abundant proteins that noncovalently bind to fibrin clots. Several of these proteins (e.g., apolipoprotein J/clusterin, haptoglobin, α2-macroglobulin, α1-antitrypsin) can act as extracellular
Jiangnan Zheng et al.
Journal of chromatography. A, 1358, 29-38 (2014-07-22)
Selective enrichment of glycopeptides from complex biological samples is essential for MS-based glycoproteomics, but challenges still remain. In this work, glucose-functionalized magnetic mesoporous nanoparticles (MMNs), which hold the attractive features of well-defined core/shell structure, high specific surface area (324m(2)/g), narrow
Tzu-Yuan Wang et al.
Food chemistry, 234, 431-438 (2017-05-30)
A total of 294 edible protein sequences and 5 commercial proteases listed in the BIOPEP database were analyzed in silico. The frequency (A), a parameter in silico described previously, was examined further to calculating the ratio of truncated peptides with
J Sotres et al.
Caries research, 48(2), 98-110 (2013-12-04)
We show in this work how systems formed by phosphoproteins on calcium phosphate surfaces can be directly characterized, in real time, in liquid medium, without the need for elution or labeling. Specifically, we show how this is possible by applying
Francesca Trimboli et al.
Journal of dairy science, 102(7), 5962-5970 (2019-05-06)
The addition of cow milk during the production of buffalo mozzarella is a common fraud in dairy industries because of the lower price and greater availability of cow milk throughout the year. The aim of this study was to develop
Benedikt W Bauer et al.
Cell, 184(21), 5448-5464 (2021-10-09)
Structural maintenance of chromosomes (SMC) complexes organize genome topology in all kingdoms of life and have been proposed to perform this function by DNA loop extrusion. How this process works is unknown. Here, we have analyzed how loop extrusion is
Mohammed Zourob et al.
Methods in molecular biology (Clifton, N.J.), 503, 89-138 (2009-01-20)
The resonant mirror (RM) biosensor is a leaky waveguide-based instrument that uses the evanescent field to probe changes in the refractive index at the sensing surface.The RM can therefore be used to monitor in real-time and label-free the interaction between
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