CN
Search Within
c6905
应用筛选条件
关键词:'c6905'
显示 1-30 共 71 条结果 关于 "c6905" 范围 论文
Simone Talens et al.
Thrombosis and haemostasis, 119(10), 1624-1631 (2019-08-23)
Many proteins bind to fibrin during clot formation in plasma. We previously identified by mass spectrometry the most abundant proteins that noncovalently bind to fibrin clots. Several of these proteins (e.g., apolipoprotein J/clusterin, haptoglobin, α2-macroglobulin, α1-antitrypsin) can act as extracellular
Hao Wang et al.
Journal of chromatography. A, 1564, 69-75 (2018-06-17)
Analysis of phosphoproteins always faces the challenge of low stoichiometry, which demands highly selective and efficient enrichment in the initial sample preparation. Here we report our synthesis of the novel titanium (IV) ion immobilized adenosine triphosphate functionalized silica nanoparticles (Ti4+-ATP-NPs)
Javier Martínez-Botas et al.
Methods in molecular biology (Clifton, N.J.), 2578, 219-236 (2022-09-25)
Peptide microarrays are a powerful tool to identify linear epitopes of food allergens in a high-throughput manner. The main advantages of the microarray-based immunoassay are as follows: the possibility to assay thousands of targets simultaneously, the requirement of a low
Julia Grawenhoff et al.
Methods in molecular biology (Clifton, N.J.), 2431, 547-568 (2022-04-13)
Motor protein-driven transport of mRNAs on microtubules and their local translation underlie important neuronal functions such as development, growth cone steering, and synaptic plasticity. While there is abundant data on how membrane-bound cargoes such as vesicles, endosomes, or mitochondria are
Andrew R Harris et al.
Nature communications, 11(1), 5973-5973 (2020-11-27)
The assembly of actin filaments into distinct cytoskeletal structures plays a critical role in cell physiology, but how proteins localize differentially to these structures within a shared cytoplasm remains unclear. Here, we show that the actin-binding domains of accessory proteins
Yuxi Deng et al.
Journal of agricultural and food chemistry, 66(16), 4219-4232 (2018-04-05)
Based on trypsin specificity (for lysines and arginines), trypsins from different sources are expected to hydrolyze a given protein to the same theoretical maximum degree of hydrolysis (DHmax,theo). This is in contrast with experiments. Using α-lactalbumin and β-casein, this study
Javier Martínez-Botas et al.
Methods in molecular biology (Clifton, N.J.), 2344, 119-135 (2021-06-12)
Peptide microarrays have been used to study protein-protein interaction, enzyme-substrate profiling, epitope mapping, vaccine development, and immuno-profiling. Unlike proteins, peptides are cheap to produce, and can be produced in a high-throughput manner, in a reliable and consistent procedure that reduces
F Valentino et al.
Soft matter, 12(47), 9429-9435 (2016-11-11)
Pulling membrane nanotubes from liposomes presents a powerful method to gain access to membrane mechanics. Here we extend classical optical tweezers studies to infer membrane nanotube dynamics with high spatial and temporal resolution. We first validate our force measurement setup
Presence of functional, autoreactive human milk-specific IgE in infants with cow's milk allergy.
Jarvinen KM., et al
Clinical and Experimental Allergy (2011)
Hannes Maib et al.
Current biology : CB, 32(13), 2821-2833 (2022-05-25)
Polarized trafficking is necessary for the development of eukaryotes and is regulated by a conserved molecular machinery. Late steps of cargo delivery are mediated by the exocyst complex, which integrates lipid and protein components to tether vesicles for plasma membrane
C Fuerer et al.
Journal of dairy science, 103(2), 1193-1207 (2019-11-25)
Infant formulations are constantly evolving as novel protein ingredients are added to make them more closely mimic the protein profile of human milk; however, precise analytical methods for characterizing and quantifying the major milk proteins in such formulations are currently
Hendrik Bruns et al.
Journal of synchrotron radiation, 30(Pt 4), 788-795 (2023-05-26)
A sample environment and manipulation tool is presented for single-particle X-ray experiments in an aqueous environment. The system is based on a single water droplet, positioned on a substrate that is structured by a hydrophobic and hydrophilic pattern to stabilize
Ivo A Telley et al.
Methods in molecular biology (Clifton, N.J.), 777, 127-145 (2011-07-21)
Several microtubule-associated proteins localize in living cells selectively to an extended region at the growing microtubule plus ends. Over the last years, these plus-end-tracking proteins, also called +TIPs, have attracted considerable interest because they are involved in a large variety
Dorota Dulko et al.
Food research international (Ottawa, Ont.), 145, 110413-110413 (2021-06-12)
The gastrointestinal hydrolysis of food proteins has been portrayed in scientific literature to predominantly depend on the activity and specificity of proteolytic enzymes. Human bile has not been considered to facilitate proteolysis in the small intestine, but rather to assist
Stefan Niekamp et al.
Nature methods, 17(4), 437-441 (2020-03-24)
Photobleaching limits extended imaging of fluorescent biological samples. We developed DNA-based 'FluoroCubes' that are similar in size to the green fluorescent protein, have single-point attachment to proteins, have a ~54-fold higher photobleaching lifetime and emit ~43-fold more photons than single
Jeongjoon Choi et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(14), 8074-8082 (2020-03-27)
The heat-stable nucleoid structuring (H-NS, also referred to as histone-like nucleoid structuring) protein silences transcription of foreign genes in a variety of Gram-negative bacterial species. To take advantage of the products encoded in foreign genes, bacteria must overcome the silencing
V Artegoitia et al.
Journal of animal physiology and animal nutrition, 97(5), 919-932 (2012-08-18)
The objective was to evaluate the effect of body condition score (BCS) at 30 days before calving (-30 days) induced by a differential nutritional management, parity and week of lactation (WOL) on milk yield and composition, and milk casein and fatty acid
Isabel Pablos et al.
Cell reports, 37(4), 109892-109892 (2021-10-22)
The main viral protease (3CLpro) is indispensable for SARS-CoV-2 replication. We delineate the human protein substrate landscape of 3CLpro by TAILS substrate-targeted N-terminomics. We identify more than 100 substrates in human lung and kidney cells supported by analyses of SARS-CoV-2-infected
Stefan Niekamp et al.
Proceedings of the National Academy of Sciences of the United States of America, 116(10), 4275-4284 (2019-02-17)
Light microscopy is a powerful tool for probing the conformations of molecular machines at the single-molecule level. Single-molecule Förster resonance energy transfer can measure intramolecular distance changes of single molecules in the range of 2 to 8 nm. However, current
T M Ellis et al.
Diabetologia, 41(6), 731-735 (1998-07-14)
Elevated cellular immune responses against the cows' milk protein beta casein have been reported in individuals with Type I diabetes mellitus, a finding supportive of the concept that cows' milk consumption may be causative for the disease. We analysed cellular
Andy Maloney et al.
PloS one, 6(6), e19522-e19522 (2011-06-16)
In this study, we report differences in the observed gliding speed of microtubules dependent on the choice of bovine casein used as a surface passivator. We observed differences in both speed and support of microtubules in each of the assays.
Fang Huang et al.
eLife, 10 (2021-11-26)
The positive transcription elongation factor b (P-TEFb) is a critical coactivator for transcription of most cellular and viral genes, including those of HIV. While P-TEFb is regulated by 7SK snRNA in proliferating cells, P-TEFb is absent due to diminished levels
Hiroshi Nakagawa et al.
Biophysical journal, 120(23), 5408-5420 (2021-11-01)
β-casein undergoes a reversible endothermic self-association, forming protein micelles of limited size. In its functional state, a single β-casein monomer is unfolded, which creates a high structural flexibility, which is supposed to play a major role in preventing the precipitation
Dóra Mosztbacher et al.
Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.], 21(2), 342-352 (2021-02-03)
Mesotrypsin is a low-abundance human trypsin isoform with a unique evolutionary mutation that conferred resistance to trypsin inhibitors and restricted substrate specificity. Mesotrypsin degrades the serine protease inhibitor Kazal type 1 (SPINK1) and thereby might increase risk for pancreatitis. Here
Bence Hajdusits et al.
eLife, 10 (2021-07-31)
In Gram-positive bacteria, the McsB protein arginine kinase is central to protein quality control, labeling aberrant molecules for degradation by the ClpCP protease. Despite its importance for stress response and pathogenicity, it is still elusive how the bacterial degradation labeling
Serena Di Palma et al.
Nature protocols, 7(11), 2041-2055 (2012-10-27)
Multidimensional liquid chromatography (LC) combined with mass spectrometry (MS) has become a standard technique in proteomics to reduce sample complexity and to tackle the dynamic range in protein abundance. Fractionation is necessary to obtain a comprehensive analysis of complex biological
Mina Dokouhaki et al.
International journal of biological macromolecules, 144, 742-750 (2019-12-15)
The Chaplin E peptide is a surface-active agent that can adsorb to the air/water interface and form interfacial films that display distinct interfacial properties as a function of pH. The ~2 nm thick homogeneous Chaplin E film formed under acidic conditions
Ayush Saurabh et al.
The journal of physical chemistry. B (2022-06-02)
Quantitative fluorescence analysis is often used to derive chemical properties, including stoichiometries, of biomolecular complexes. One fundamental underlying assumption in the analysis of fluorescence data─whether it be the determination of protein complex stoichiometry by super-resolution, or step-counting by photobleaching, or
Jeanette Lund Madsen et al.
Clinical and translational allergy, 4, 25-25 (2014-09-11)
Characterisation of the specific antibody response, including the epitope binding pattern, is an essential task for understanding the molecular mechanisms of food allergy. Examination of antibody formation in a controlled environment requires animal models. The purpose of this study was
Mikhail M Vorob'ev
International journal of molecular sciences, 23(15) (2022-07-29)
The opening of protein substrates during degradation by proteases and the corresponding exposure of their internal peptide bonds for a successful enzymatic attack, the so-called demasking effect, was studied for β-lactoglobulin (β-LG) and β-casein (β-CN) hydrolyzed by trypsin. Demasking was
1/3