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关键词:'C7880'
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Ya-Ling Huang et al.
International journal of biological macromolecules, 124, 796-801 (2018-12-01)
Our previous study suggested that extrusion can enhance the soluble dietary fiber (SDF) and improve the physicochemical properties of fiber-rich orange pomace (OP). The aim of this study was to determine the predictive indices for the hypoglycemic, hypocholesterolemic, and fermentation
Savvas Saouros et al.
Scientific reports, 11(1), 12328-12328 (2021-06-12)
Boron has essential roles in plant growth and development. BOR proteins are key in the active uptake and distribution of boron, and regulation of intracellular boron concentrations. However, their mechanism of action remains poorly studied. BOR proteins are homologues of
Charles Ansong et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(25), 10153-10158 (2013-05-31)
Characterization of the mature protein complement in cells is crucial for a better understanding of cellular processes on a systems-wide scale. Toward this end, we used single-dimension ultra-high-pressure liquid chromatography mass spectrometry to investigate the comprehensive "intact" proteome of the
Hanan R Shehata et al.
Frontiers in microbiology, 11, 1095-1095 (2020-06-26)
Probiotics are defined as "live microorganisms that, when administered in adequate amounts, confer a health benefit on the host." The diverse health benefits have contributed to rapid increase in probiotic consumption and in the value of probiotic market, valued at
Zrinka Karačić et al.
Biological chemistry, 393(12), 1523-1532 (2013-05-15)
Human dipeptidyl peptidase III (DPP III) is a member of the metallopeptidase family M49, involved in protein metabolism and oxidative stress response. DPPIII crystal structure shows the two lobe-like domains separated by a wide cleft. The human enzyme has a
Rana Barakat et al.
MicrobiologyOpen, 3(1), 1-14 (2013-12-07)
The blue-green phenazine, Pyocyanin (PYO), is a well-known virulence factor produced by Pseudomonas aeruginosa, notably during cystic fibrosis lung infections. It is toxic to both eukaryotic and bacterial cells and several mechanisms, including the induction of oxidative stress, have been
Michael Hellwig et al.
Journal of agricultural and food chemistry, 63(30), 6723-6730 (2015-07-18)
Maillard reaction products (MRPs) are taken up in substantial amounts with the daily diet, but the majority are not transported across the intestinal epithelium. The aim of this study was to obtain first insights into the stability of dietary MRPs
Gustavo H C Varca et al.
Drug development and industrial pharmacy, 41(3), 430-435 (2014-01-15)
The performance of the standardized extrusion-spheronization technique, operational conditions, formulation parameters and storage of the final product over the bioactivity of papain containing pellets has been evaluated to obtain an insight into the potential of the technique for the manufacture
Stuart J Smith et al.
Nature protocols, 1(5), 2195-2203 (2007-04-05)
The stable integration of transgenes into embryos of the frog Xenopus laevis is achieved using the procedure described here. Linear DNA containing the transgene is incorporated randomly into sperm nuclei that have had their membranes disrupted with detergent treatment. Microinjection
Emily A Peluso et al.
Current protocols in microbiology, 57(1), e104-e104 (2020-06-17)
Considered a commensal, the Gram-negative anaerobe Fusobacterium nucleatum is a key member of the oral microbiome due to its wide range of interactions with many oral microbes. While the periodontal pathogenic properties of this organism have widely been examined, its
Pengbo Zhou
Methods in molecular biology (Clifton, N.J.), 284, 67-77 (2004-06-03)
Controlling the stability of cellular proteins is a fundamental way by which cells regulate growth, differentiation, survival, and development. Measuring the turnover rate of a protein is often the first step in assessing whether or not the function of a
Nitric oxide quenches the fire in heart mitochondria.
Paul T Schumacker
Nature medicine, 19(6), 666-667 (2013-06-08)
Microsurgical Manipulations to Isolate Collectively Migrating Mesendoderm.
Davidson
CSH Protocols, 2022, Pdb-Pdb (2023)
Borna Ghosh et al.
The Journal of biological chemistry, 288(24), 17420-17431 (2013-05-04)
General anesthetics exert many of their CNS actions by binding to and modulating membrane-embedded pentameric ligand-gated ion channels (pLGICs). The structural mechanisms underlying how anesthetics modulate pLGIC function remain largely unknown. GLIC, a prokaryotic pLGIC homologue, is inhibited by general
Biochemistry: Oxidation controls the DUB step.
Michael J Clague
Nature, 497(7447), 49-50 (2013-05-03)
[Targeted effect profile - proven effectiveness].
MMW Fortschritte der Medizin, 155(5), 70-71 (2013-05-10)
Laurine Legroux et al.
Journal of neuroscience methods, 247, 23-31 (2015-03-31)
Flow cytometry is an efficient and powerful technique to characterize and quantify numerous cells. However, the strengths of this technique have not been widely harnessed in neurosciences due to the critical step of CNS tissue preparation into a single cell
Christopher M Jenkins et al.
Biochemistry, 52(24), 4250-4263 (2013-05-25)
The multifaceted roles of calcium-independent phospholipase A2β (iPLA2β) in numerous cellular processes have been extensively examined through utilization of the iPLA2-selective inhibitor (E)-6-(bromomethylene)-3-(1-naphthalenyl)-2H-tetrahydropyran-2-one (BEL). Herein, we employed accurate mass/high resolution mass spectrometry to demonstrate that the active site serine (S465)
A F Vanin et al.
Biofizika, 58(1), 126-136 (2013-05-09)
Current notions and new experimental data of the authors on physico-chemical features of dinitrosyl iron complexes with natural thiol-containing ligands (glutathione or cysteine), underlying the ability of the complexes to act as NO molecule and nitrosonium ion donors, are considered.
Mingdeng Liu et al.
Water science and technology : a journal of the International Association on Water Pollution Research, 67(11), 2444-2450 (2013-06-12)
In the present work, cysteine-modified orange peel (COP) for the removal of Cu(II) from aqueous solutions has been developed and comparatively studied with diethylenetriamine-modified orange peel (DOP). Both COP and DOP were systematically evaluated via their capabilities for adsorbing Cu(II)
Pramod Kumar Yadav et al.
The Journal of biological chemistry, 288(27), 20002-20013 (2013-05-24)
Mercaptopyruvate sulfurtransferase (MST) is a source of endogenous H2S, a gaseous signaling molecule implicated in a wide range of physiological processes. The contribution of MST versus the other two H2S generators, cystathionine β-synthase and γ-cystathionase, has been difficult to evaluate
Cécile Chenot et al.
Journal of agricultural and food chemistry, 67(14), 4002-4010 (2019-03-16)
After evidence of the cysteinylated precursors of 3-sulfanyl-4-methylpentan-1-ol (Cys-26) and 3-sulfanylhexan-1-ol (Cys-23) in hop, S-glutathione precursors (G-23 and G-26) were recently discovered in different dual-purpose hop varieties. Because free 3-sulfanylpentan-1-ol (21) has also been detected in hop, the present work
Bianca Johansen et al.
Antibiotics (Basel, Switzerland), 9(7) (2020-07-12)
Tea Tree oil (TTO) is well known for its numerous good properties but might be also irritating or toxic when used topically or ingested, thus limiting the number of possible applications in Humans. The aim of the study was to
Markus Mund et al.
Cell, 174(4), 884-896 (2018-07-31)
Clathrin-mediated endocytosis is an essential cellular function in all eukaryotes that is driven by a self-assembled macromolecular machine of over 50 different proteins in tens to hundreds of copies. How these proteins are organized to produce endocytic vesicles with high precision
Establishment, maintenance, and transfection of in vitro cultures of human retinal pigment epithelium.
Martin J Stevens et al.
Methods in molecular medicine, 107, 343-351 (2004-10-20)
David A Lee et al.
Methods in molecular medicine, 100, 307-324 (2004-07-29)
Mechanical loading of chondrocytes in 3D constructs has been used to investigate mechanotransduction and its potential for stimulating tissue-engineered cartilage repair. This chapter describes the preparation of 3D agarose or alginate constructs seeded with isolated chondrocytes and specific test rigs
Sandrine Brugiroux et al.
Current protocols, 2(9), e548-e548 (2022-09-13)
The oligo-mouse-microbiota (OMM12 ) is a widely used syncom that colonizes gnotobiotic mice in a stable manner. It provides several fundamental functions to its murine host, including colonization resistance against enteric pathogens. Here, we designed and validated specific fluorescence in
D J Powell et al.
Poultry science, 92(8), 2163-2173 (2013-07-23)
Early posthatch satellite cell (SC) mitotic activity is a critical component of muscle development and growth. Satellite cells are stem cells that can be induced by nutrition to follow other cellular developmental pathways. The objective of the current study was
Kinsley Tate et al.
International journal of molecular sciences, 22(19) (2021-10-14)
The developing prenatal brain is particularly susceptible to environmental disturbances. During prenatal brain development, synapses form between neurons, resulting in neural circuits that support complex cognitive functions. In utero exposure to environmental factors such as pharmaceuticals that alter the process
Nina Meyer et al.
Autophagy, 17(11), 3424-3443 (2021-01-20)
Increasing evidence suggests that induction of lethal macroautophagy/autophagy carries potential significance for the treatment of glioblastoma (GBM). In continuation of previous work, we demonstrate that pimozide and loperamide trigger an ATG5- and ATG7 (autophagy related 5 and 7)-dependent type of
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