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Masahiro Yoshizawa-Fujita et al.
The journal of physical chemistry. B, 114(49), 16373-16380 (2010-11-23)
We describe zwitterion, 3-(1-butyl-1H-imidazol-3-ium-3-yl)propane-1-sulfonate (Bimps), mixtures with 1,1,1-trifluoro-N-(trifluoromethylsulfonyl)methanesulfoneamide (HN(Tf)(2)) as new proton transport electrolytes. We report proton transport mechanisms in the mixtures based on results from several methods including thermal analyses, the complex-impedance method, and the pulsed field gradient spin
Michaela Herrmann et al.
European biophysics journal : EBJ, 44(7), 503-512 (2015-05-29)
Bilayer mechanical properties are not only of crucial importance to the mechanism of action of mechanosensation in lipid membranes but also affect preparative laboratory tasks such as membrane-protein refolding. We report this for coupled refolding and bilayer insertion of outer membrane
E H Chowdhury et al.
Journal of biotechnology, 119(4), 343-347 (2005-08-13)
Purification of plasmid DNA from bacteria is an essential tool in recombinant DNA technology and has become an essential task in laboratories to industries. Moreover, the recent progress of "Gene therapy" and "Genetic vaccination" also demands production of pharmaceutical grade
Henrik Everberg et al.
Methods in molecular biology (Clifton, N.J.), 528, 73-81 (2009-01-21)
Identification and characterization of membrane proteins is of increasing importance in modern proteomic studies. It is of central interest to have access to methods that combine efficient solubilization with enrichment of proteins and intact protein complexes. Separation methods have been
Georgia L Isom et al.
Cell, 181(3), 653-664 (2020-05-04)
Gram-negative bacteria are surrounded by an outer membrane composed of phospholipids and lipopolysaccharide, which acts as a barrier and contributes to antibiotic resistance. The systems that mediate phospholipid trafficking across the periplasm, such as MCE (Mammalian Cell Entry) transporters, have
B Fricke
Analytical biochemistry, 281(2), 144-150 (2000-06-28)
Zwitterionic detergents interfere with the salt-induced phase separation for nonionic detergents in a concentration-dependent manner by shifting the normal cloud point of nonionic detergents to a higher ionic strength at room temperature. This phenomenon was used to determine the concentration
Yoshiko Moriyama et al.
Journal of oleo science, 60(5), 229-236 (2011-04-20)
The secondary structure of bovine serum albumin (BSA) in the binary surfactant system of anionic sodium dodecyl sulfate (SDS) and zwitterionic N-dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate (DDAPS) was examined at 25°C. The helicity of BSA decreased from 66% to 55% in a solution of
Suguru Yamamoto et al.
Biochemistry, 43(34), 11075-11082 (2004-08-25)
In beta(2)-microglobulin-related (Abeta2M) amyloidosis, partial unfolding of beta(2)-microglobulin (beta2-m) is believed to be prerequisite to its assembly into Abeta2M amyloid fibrils in vivo. Although low pH or 2,2,2-trifluoroethanol at a low concentration has been reported to induce partial unfolding of
Hongying Jia et al.
Journal of chromatography. A, 1143(1-2), 88-97 (2007-02-03)
With the accelerating development of new drugs, there is a high need for rapid and simple screening technologies. In this paper, a new in vitro method, dodecyl dimethyl betaine (BS-12) micellar liquid chromatography (MLC) was presented for prediction of protein-drug
J W Anderson et al.
Biochemistry, 39(40), 12200-12209 (2000-10-04)
Bacterial cell walls are cross-linked in the final step of biosynthesis by specific D-alanyl-D-alanine(DD)-peptidases/transpeptidases. The natural substrates of these enzymes should therefore be segments of peptidoglycan, but high specificity for such structures has yet to be demonstrated. The binding of
R R Julian et al.
Journal of the American Chemical Society, 123(15), 3577-3583 (2001-07-27)
The discovery of several new unusually stable aggregates of arginine that are intermolecularly bound by salt bridges is reported. Quadrupole ion-trap mass spectrometry provides evidence for the stability of arginine in the zwitterionic state, where the protonated guanidinium group of
Abhay H Pande et al.
Biochemistry, 43(46), 14653-14666 (2004-11-17)
Mammalian 5-lipoxygenase (5-LO) catalyzes the conversion of arachidonic acid (AA) to leukotrienes, potent inflammatory mediators. 5-LO is activated by a Ca(2+)-mediated translocation to membranes, and demonstrates the characteristic features of interfacially activated enzymes, yet the mechanism of membrane binding of
Paul L Sorgen et al.
Biochemistry, 41(1), 31-41 (2002-01-05)
The light harvesting 1 antenna (LH1) complex from Rhodobacter sphaeroides funnels excitation energy to the photosynthetic reaction center. Our ultimate goal is to build up the structure of LH1 from structures of its individual subunits, much as the antenna can
Beatriz Martín-García et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 13(16), 3682-3690 (2012-08-15)
Films of a few layers in thickness of reduced graphite oxide (RGO) sheets functionalized by the zwitterionic surfactant N-dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate (DDPS) are obtained by using the Langmuir-Blodgett method. The quality of the RGO sheets is checked by analyzing the degrees of
U Boronowsky et al.
Biochimica et biophysica acta, 1506(1), 55-66 (2001-06-22)
Cytochrome (cyt) b-c complexes play a central role in electron transfer chains and are almost ubiquitous in nature. Although similar in their basic structure and function, the cyt b(6)f complex of photosynthetic membranes and its counterpart, the mitochondrial cyt bc(1)
H L Liou et al.
Biochemistry, 40(21), 6475-6485 (2001-05-24)
The tertiary structure of murine adipocyte fatty acid-binding protein (AFABP) is a flattened 10-stranded beta-barrel capped by a helix-turn-helix segment. This helical domain is hypothesized to behave as a "lid" or portal for ligand entry into and exit from the
María Muñoz et al.
Langmuir : the ACS journal of surfaces and colloids, 20(25), 10858-10867 (2004-12-01)
Micellization in sodium dodecyl sulfate (SDS)-N-dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate and SDS-polyoxyethylenesorbitan monolaurate binary surfactant solutions was studied by means of conductivity and surface tension measurements. These studies showed that two types of micellar aggregates are present in the mixed micellar solutions. Two reactions
Feng He et al.
Journal of pharmaceutical sciences, 98(9), 3340-3357 (2008-12-17)
The hepatitis C virus (HCV) envelope glycoprotein E1 has been widely employed as a potential vaccine antigen in clinical research. A truncated form (amino acids 192-326) of the E1 protein (E1y) was expressed in the yeast Hansenula polymorpha and purified
W Hu et al.
The Analyst, 125(3), 447-451 (2000-06-01)
A new ion chromatographic (IC) system, which uses zwitterionic (e.g., Zwittergent 3-14) micelles as both stationary and mobile phases, highly useful for the analysis of inorganic anions in biological samples, was developed. The zwitterionic micellar stationary phase (which is obtained
Mónica Ferrit et al.
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 31(3-4), 211-220 (2007-05-15)
In this research, the effects of micellar systems on alkaline hydrolysis reactions of acetylsalicylic acid (ASA) and triflusal (TFL) were found to be dependant upon the surfactant charge within the micelle. In cationic micelles, there is a catalytic effect at
Marien I de Jonge et al.
European journal of biochemistry, 269(21), 5215-5223 (2002-10-24)
Opacity-associated (Opa) proteins are outer membrane proteins which play a critical role in the adhesion of pathogenic Neisseria spp. to epithelial and endothelial cells and polymorphonuclear neutrophils. The adherence is mainly mediated by the CD66-epitope-containing members of the carcinoembryonic-antigen family
Darryl Chapman et al.
Analytical biochemistry, 303(2), 153-166 (2002-04-13)
The membrane-destabilizing properties of Amphotericin B and Zwittergent were used as benchmark compounds for examining in detail their membrane-altering effects in a series of human red blood cell lysis assays. The procedures included examining dose responses and the effects of
Interaction between ionic liquid and zwitterionic surfactant: A comparative study of two ionic liquids with different anions
Behera, K. and S. Pandey
Journal of Colloid and Interface Science, 331, 10-10 (2009)
S Orlowski et al.
Cancer biochemistry biophysics, 16(1-2), 85-110 (1999-01-29)
P-glycoprotein (P-gp), a plasma membrane glycoprotein associated with the multidrug resistance phenotype, is responsible for the ATP-dependent efflux of various amphiphilic drugs. Using membrane vesicles prepared from the multidrug resistant cell line DC-3F/ADX, we studied the perturbation of the basal
Cecile Ancey et al.
Cardiovascular research, 59(1), 78-85 (2003-06-28)
Recent in vivo and in vitro studies in animals have demonstrated that cytokines of the IL-6 family are involved in cardiac hypertrophy and in protection of cardiomyocytes against apoptosis. The present study aims to analyse the capacity of human atrial
Habauka M Kwaambwa et al.
Biopolymers, 97(4), 209-218 (2011-12-16)
Protein extracted from Moringa oleifera (MO) seeds has been advocated as a cheap and environmental friendly alternative to ionic flocculants for water purification. However, the nature and mechanism of its interaction with particles in water, as well as with dissolved
Zhan-guang Chen et al.
Guang pu xue yu guang pu fen xi = Guang pu, 25(10), 1680-1683 (2006-01-07)
A new method has been developed for the determination of DNA by resonance light scattering with dodecyl dimethyl betaine (BS-12) in aqueous solution as a new probe. At pH 9.3, the interactions of BS-12 and DNA gave strong RLS signals
Nidhi Puri et al.
European journal of medicinal chemistry, 45(11), 4813-4826 (2010-08-27)
In this study, we have explored the structure activity relationships of substrates of two major, promiscuous, multidrug transporters of an opportunistic human pathogen Candida albicans namely, CaCdr1p and CaMdr1p. To differentiate between substrates and non-substrates, the susceptibilities of the Saccharomyces
D Boehning et al.
The EMBO journal, 19(20), 5450-5459 (2000-10-18)
Inositol 1,4,5-trisphosphate receptors (IP(3)Rs) are a family of intracellular Ca(2+) channels that exist as homo- or heterotetramers. In order to determine whether the N-terminal ligand-binding domain is in close physical proximity to the C-terminal pore domain, we prepared microsomal membranes
Lau Khye Tee et al.
Plasmid, 66(1), 38-46 (2011-03-23)
Plasmid DNA is one of the indispensable components in molecular biology research and a potential biomaterial for gene therapy and DNA vaccination. Both quality and quantity of extracted plasmid DNA are of the great interests in cloning and subsequent expression
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