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P A Ropp et al.
Genomics, 36(3), 449-458 (1996-09-15)
The nuclear-encoded DNA polymerase gamma (DNA POL gamma) is the sole DNA polymerase required for the replication of the mitochondrial DNA. We have cloned the cDNA for human DNA POL gamma and have mapped the gene to the chromosomal location
O Domínguez et al.
The EMBO journal, 19(7), 1731-1742 (2000-04-04)
A novel DNA polymerase has been identified in human cells. Human DNA polymerase mu (Pol mu), consisting of 494 amino acids, has 41% identity to terminal deoxynucleotidyltransferase (TdT). Human Pol mu, overproduced in Escherichia coli in a soluble form and
Francesca Incardona et al.
eLife, 4, e06938-e06938 (2015-06-19)
The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by replicating selected results from a substantial number of high-profile papers in the field of cancer biology published between 2010 and 2012. This Registered report
Chunaram Choudhary et al.
Science (New York, N.Y.), 325(5942), 834-840 (2009-07-18)
Lysine acetylation is a reversible posttranslational modification of proteins and plays a key role in regulating gene expression. Technological limitations have so far prevented a global analysis of lysine acetylation's cellular roles. We used high-resolution mass spectrometry to identify 3600
Patrick Forterre
Journal of molecular biology, 425(23), 4714-4726 (2013-10-01)
The replicon model has initiated a major research line in molecular biology: the study of DNA replication mechanisms. Until now, the majority of studies have focused on a limited set of model organisms, mainly from Bacteria or Opisthokont eukaryotes (human
P E Gibbs et al.
Proceedings of the National Academy of Sciences of the United States of America, 95(12), 6876-6880 (1998-06-17)
To get a better understanding of mutagenic mechanisms in humans, we have cloned and sequenced the human homolog of the Saccharomyces cerevisiae REV3 gene. The yeast gene encodes the catalytic subunit of DNA polymerase zeta, a nonessential enzyme that is
Purification and properties of deoxyribonucleic acid polymerase from Micrococcus lysodeikticus.
B K Zimmerman
The Journal of biological chemistry, 241(9), 2035-2041 (1966-05-10)
Amy Reeve et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 33(26), 10790-10801 (2013-06-28)
Mitochondrial defects within substantia nigra (SN) neurons are implicated in the pathogenesis of Parkinson's disease. SN neurons show increased mitochondrial defects, mitochondrial DNA deletion levels, and susceptibility to such dysfunction, although the role of mitochondria in neuronal degeneration remains uncertain.
A Chien et al.
Journal of bacteriology, 127(3), 1550-1557 (1976-09-01)
A stable deoxyribonucleic acid (DNA) polymerase (EC 2.7.7.7) with a temperature optimum of 80 degrees C has been purified from the extreme thermophile Thermus aquaticus. The enzyme is free from phosphomonoesterase, phosphodiesterase and single-stranded exonuclease activities. Maximal activity of the
[The effect of Luteococcus japonicus subsp. Casei reactivating factor on the expression of SOS response genes].
N G Loĭko et al.
Mikrobiologiia, 82(2), 139-146 (2013-07-03)
Fadime Eroglu et al.
The American journal of tropical medicine and hygiene, 91(5), 895-900 (2014-09-17)
This study aimed at finding out the most effective clinical samples and methods in chronic cutaneous leishmaniasis (CCL). Smear, aspiration fluid, and filter paper samples were taken from 104 skin lesions of suspected cases with CCL, and they were compared
Sharon Gauci et al.
Analytical chemistry, 81(11), 4493-4501 (2009-05-06)
The analysis of proteome-wide phosphorylation events is still a major analytical challenge because of the enormous complexity of protein phosphorylation networks. In this work, we evaluate the complementarity of Lys-N, Lys-C, and trypsin with regard to their ability to contribute
Milind K Gupta et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(18), 7252-7257 (2013-04-17)
Replication fork pausing drives genome instability, because any loss of paused replisome activity creates a requirement for reloading of the replication machinery, a potentially mutagenic process. Despite this importance, the relative contributions to fork pausing of different replicative barriers remain
K C Walls et al.
Journal of neuropathology and experimental neurology, 68(12), 1326-1338 (2009-11-17)
Perinatal hypoxia-ischemia may result in long-term neurological deficits. In addition to producing neuron death, HI causes death of neural precursor cells (NPCs) in the developing brain. To characterize the molecular pathways that regulate hypoxia-induced death of NPCs, we treated a
Patricia L Kannouche et al.
Molecular cell, 14(4), 491-500 (2004-05-20)
Most types of DNA damage block replication fork progression during DNA synthesis because replicative DNA polymerases are unable to accommodate altered DNA bases in their active sites. To overcome this block, eukaryotic cells employ specialized translesion synthesis (TLS) polymerases, which
Yang Liu et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(5), 1789-1794 (2014-01-23)
DNA polymerase κ (Polκ) is the only known Y-family DNA polymerase that bypasses the 10S (+)-trans-anti-benzo[a]pyrene diol epoxide (BPDE)-N(2)-deoxyguanine adducts efficiently and accurately. The unique features of Polκ, a large structure gap between the catalytic core and little finger domain
S Aoufouchi et al.
Nucleic acids research, 28(18), 3684-3693 (2000-09-13)
We describe here two novel mouse and human DNA polymerases: one (pol lambda) has homology with DNA polymerase beta while the other one (pol mu) is closer to terminal deoxynucleotidyltransferase. However both have DNA polymerase activity in vitro and share
DNA repair: Familiar ends with alternative endings.
Nam Woo Cho et al.
Nature, 518(7538), 174-176 (2015-02-03)
Raphael Ceccaldi et al.
Nature, 518(7538), 258-262 (2015-02-03)
Large-scale genomic studies have shown that half of epithelial ovarian cancers (EOCs) have alterations in genes regulating homologous recombination (HR) repair. Loss of HR accounts for the genomic instability of EOCs and for their cellular hyper-dependence on alternative poly-ADP ribose
How DNA polymerases catalyze DNA replication, repair, and mutation.
Ming-Daw Tsai
Biochemistry, 53(17), 2749-2751 (2014-04-11)
Sean M Post et al.
Nucleic acids research, 31(19), 5568-5575 (2003-09-23)
The checkpoint Rad proteins Rad17, Rad9, Rad1, Hus1, ATR, and ATRIP become associated with chromatin in response to DNA damage caused by genotoxic agents and replication inhibitors, as well as during unperturbed DNA replication in S phase. Here we show
Maryam Khalaj et al.
The Journal of biological chemistry, 289(6), 3811-3824 (2013-12-21)
Repro22 is a mutant mouse produced via N-ethyl-N-nitrosourea-induced mutagenesis that shows sterility with germ cell depletion caused by defective proliferation of primordial germ cells, decreased body weight, and partial lethality during embryonic development. Using a positional cloning strategy, we identified
Moneeb Ehtesham et al.
Cancer letters, 274(2), 305-312 (2008-11-15)
Increasing evidence points to a fundamental role for cancer stem cells (CSC) in the initiation and propagation of many tumors. As such, in the context of glioblastoma multiforme (GBM), the development of treatment strategies specifically targeted towards CSC-like populations may
T Kesti et al.
The Journal of biological chemistry, 268(14), 10238-10245 (1993-05-15)
The cDNA encoding the catalytic polypeptide of human DNA polymerase epsilon was cloned. The deduced amino acid sequence reveals that the catalytic polypeptide is 2257 amino acids in length and its calculated molecular mass is 258 kDa. A single RNA
Shuhei Matsuoka et al.
Science (New York, N.Y.), 316(5828), 1160-1166 (2007-05-26)
Cellular responses to DNA damage are mediated by a number of protein kinases, including ATM (ataxia telangiectasia mutated) and ATR (ATM and Rad3-related). The outlines of the signal transduction portion of this pathway are known, but little is known about
D Huang et al.
The Biochemical journal, 339 ( Pt 3), 657-665 (1999-04-24)
The catalytic subunit of human DNA polymerase epsilon, an enzyme involved in nuclear DNA replication and repair, is encoded by the POLE1 gene. This gene is composed of 51 exons spanning at least 97 kb of genomic DNA. It was
Yong-Sam Jung et al.
Molecular and cellular biology, 31(19), 3997-4006 (2011-07-28)
Polymerase eta (PolH) is necessary for translesion DNA synthesis, and PolH deficiency predisposes xeroderma pigmentosum variant (XPV) patients to cancer. Due to the critical role of PolH in translesion DNA synthesis, the activity of PolH is tightly controlled and subjected
Ryan E Davey et al.
Stem cells (Dayton, Ohio), 24(11), 2538-2548 (2006-07-11)
Highly ordered aggregates of cells, or niches, regulate stem cell fate. Specific tissue location need not be an obligatory requirement for a stem cell niche, particularly during embryogenesis, where cells exist in a dynamic environment. We investigated autoregulatory fixed-location-independent processes
Hui Yuan et al.
Nucleic acids research, 41(11), 5817-5826 (2013-04-23)
Replicative DNA polymerases require an RNA primer for leading and lagging strand DNA synthesis, and primase is responsible for the de novo synthesis of this RNA primer. However, the archaeal primase from Pyrococcus furiosus (Pfu) frequently incorporates mismatched nucleoside monophosphate
Karen H Chang et al.
Biotechnology and bioengineering, 88(3), 287-298 (2004-10-16)
Embryonic stem (ES) cells have attracted much attention as a possible source of functional cells for regenerative medicine. Therapeutic use of ES cells requires control over the types and frequencies of cells generated during their in vitro differentiation. Due to
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