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Boyang Hua et al.
Nature methods, 11(12), 1233-1236 (2014-10-13)
We report a surface passivation method based on dichlorodimethylsilane (DDS)-Tween-20 for in vitro single-molecule studies, which, under the conditions tested here, more efficiently prevented nonspecific binding of biomolecules than the standard poly(ethylene glycol) surface. The DDS-Tween-20 surface was simple and
Gianfrancesco Ferrari et al.
Brain : a journal of neurology, 128(Pt 4), 723-731 (2005-02-04)
We studied nine infant patients with a combination of progressive neurological and hepatic failure. Eight children, including two sibling pairs and four singletons, were affected by Alpers' hepatopathic poliodystrophy. A ninth baby patient suffered of a severe floppy infant syndrome
S J Humphray et al.
Nature, 429(6990), 369-374 (2004-05-28)
Chromosome 9 is highly structurally polymorphic. It contains the largest autosomal block of heterochromatin, which is heteromorphic in 6-8% of humans, whereas pericentric inversions occur in more than 1% of the population. The finished euchromatic sequence of chromosome 9 comprises
Marzena Bienko et al.
Molecular cell, 37(3), 396-407 (2010-02-18)
DNA polymerase eta is a Y family polymerase involved in translesion synthesis (TLS). Its action is initiated by simultaneous interaction between the PIP box in pol eta and PCNA and between the UBZ in pol eta and monoubiquitin attached to
José F Ruiz et al.
Nucleic acids research, 31(15), 4441-4449 (2003-07-31)
DNA polymerase mu (Pol mu) is a novel family X DNA polymerase that has been suggested to play a role in micro-homology mediated joining and repair of double strand breaks. We show here that human Pol mu is not able
Joseph T P Yeeles et al.
Molecular cell, 52(6), 855-865 (2013-11-26)
The E. coli replisome stalls transiently when it encounters a lesion in the leading-strand template, skipping over the damage by reinitiating replication at a new primer synthesized downstream by the primase. We report here that template unwinding and lagging-strand synthesis
Koichi Ogami et al.
Biochemical and biophysical research communications, 432(1), 135-140 (2013-02-05)
Non-canonical poly(A) polymerases (ncPAPs) catalyze the addition of poly(A) tail to the 3' end of RNA to play pivotal roles in the regulation of gene expression and also in quality control. Here we identified a novel isoform of the 7th
Uracil DNA glycosylase BKRF3 contributes to Epstein-Barr virus DNA replication through physical interactions with proteins in viral DNA replication complex.
Su MT, Liu IH, Wu CW, et al.
Journal of Virology, 88(16), 8883-8899 (2014)
S S Lee et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(17), 7882-7886 (1995-08-15)
The herpes simplex virus 1 (HSV-1) genome encodes seven polypeptides that are required for its replication. These include a heterodimeric DNA polymerase, a single-strand-DNA-binding protein, a heterotrimeric helicase/primase, and a protein (UL9 protein) that binds specifically to an HSV-1 origin
Jenny E Emerson et al.
Molecular microbiology, 74(3), 541-556 (2009-08-07)
Clostridium difficile is a nosocomial pathogen that can cause severe gastrointestinal infections. C. difficile encodes a family of cell wall proteins, some of which are implicated in pathogenesis. Here we have characterized CwpV, the largest member of this family. CwpV
G Van Goethem et al.
Nature genetics, 28(3), 211-212 (2001-06-30)
Progressive external ophthalmoplegias (PEO) characterized by accumulation of large-scale mitochondrial DNA (mtDNA) deletions are rare human diseases. We mapped a new locus for dominant PEO at 15q22-q26 in a Belgian pedigree and identified a heterozygous mutation (Y955C) in the polymerase
T A Patterson et al.
Protein expression and purification, 18(1), 100-110 (2000-01-29)
The cDNA encoding the human polymerase beta from HeLa cells was PCR amplified and cloned, and its nucleotide sequence determined. The DNA sequence is identical to the polymerase beta cDNA sequence from Tera-2 cells. Three expression strategies were employed that
Gaili Wang et al.
Archives of virology, 159(5), 915-920 (2013-11-02)
Orf, which is caused by orf virus (ORFV), is distributed worldwide and is endemic in most sheep- and/or goat-raising countries. RNA interference (RNAi) pathways have emerged as important regulators of virus-host cell interactions. In this study, the specific effect of
Valérie Bergoglio et al.
The Journal of cell biology, 201(3), 395-408 (2013-04-24)
Human DNA polymerase η (Pol η) is best known for its role in responding to UV irradiation-induced genome damage. We have recently observed that Pol η is also required for the stability of common fragile sites (CFSs), whose rearrangements are
K Nagasawa et al.
The Journal of biological chemistry, 275(40), 31233-31238 (2000-07-11)
The BRCA1 COOH terminus (BRCT) motif is present in many nuclear proteins that contribute to cell cycle regulation or DNA repair. Polymerase chain reaction-based screening with degenerate primers targeted to the BRCT motif resulted in the isolation of a human
Keith E Herold et al.
Methods in molecular biology (Clifton, N.J.), 504, 441-458 (2009-01-23)
A prototype handheld, compact, rapid thermocycler was developed for multiplex analysis of nucleic acids in an inexpensive, portable configuration. Instead of the commonly used Peltier heating/cooling element, electric thin-film resistive heater and a miniature fan enable rapid heating and cooling
Joseph M Krahn et al.
Structure (London, England : 1993), 11(1), 121-127 (2003-01-09)
Oxidative damage to DNA generates 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG). During DNA replication and repair synthesis, 8-oxodG can pair with cytosine or adenine. The ability to accurately replicate through this lesion depends on the DNA polymerase. We report the first structure of a
Jian Li et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(19), 7672-7677 (2013-04-24)
Proliferating cell nuclear antigen (PCNA) is required for DNA homologous recombination (HR), but its exact role is unclear. Here, we investigated the loading of PCNA onto a synthetic D-loop (DL) intermediate of HR and the functional interactions of PCNA with
Brian A Maxwell et al.
Biochemistry, 53(17), 2804-2814 (2014-04-11)
The kinetic mechanisms by which DNA polymerases catalyze DNA replication and repair have long been areas of active research. Recently discovered Y-family DNA polymerases catalyze the bypass of damaged DNA bases that would otherwise block replicative DNA polymerases and stall
Y Dobashi et al.
Human genetics, 95(4), 389-390 (1995-04-01)
Recently, evidence has accumulated that mutations in DNA repair genes might be associated with certain steps in carcinogenesis. The DNA polymerase beta gene is one of the DNA repair genes, and mutations in it have been detected in 83% of
Nikolay Tsanov et al.
Nucleic acids research, 42(6), 3692-3706 (2014-01-16)
Proliferating cell nuclear antigen (PCNA) is a well-known scaffold for many DNA replication and repair proteins, but how the switch between partners is regulated is currently unclear. Interaction with PCNA occurs via a domain known as a PCNA-Interacting Protein motif
Daisuke Kawashima et al.
Journal of virology, 87(11), 6482-6491 (2013-04-05)
Epstein-Barr virus (EBV) replication proteins are transported into the nucleus to synthesize viral genomes. We here report molecular mechanisms for nuclear transport of EBV DNA polymerase. The EBV DNA polymerase catalytic subunit BALF5 was found to accumulate in the cytoplasm
W Lin et al.
Mutation research, 433(2), 89-98 (1999-04-02)
DNA damage can cause mutations which in turn may lead to carcinogenesis. In the yeast Saccharomyces cerevisiae, DNA damage-induced mutagenesis pathway requires the REV3 gene. It encodes the catalytic subunit of DNA polymerase zeta that specifically functions in translesion DNA
Kavitha Sivasubramaiyan et al.
Journal of biomedical science, 16, 88-88 (2009-09-24)
Trophoblast differentiation and formation of the placenta are important events linked to post-implantation embryonic development. Models mimicking the biology of trophoblast differentiation in a post-implantation maternal microenvironment are needed for understanding disorders like placental-ischemia or for applications in drug-screening, and
A Agostino et al.
Neurology, 60(8), 1354-1356 (2003-04-23)
To verify the impact of mutations in ANT1, Twinkle, and POLG1 genes in sporadic progressive external ophthalmoplegia associated with multiple mitochondrial DNA (mtDNA) deletions, DNA samples from 15 Italian and 12 British patients were screened. Mutations in ANT1 were found
K A Braun et al.
Biochemistry, 36(28), 8443-8454 (1997-07-15)
Replication Protein A (RPA) from human cells is a stable complex of 70-, 32-, and 14-kDa subunits that is required for multiple processes in DNA metabolism. RPA binds with high affinity to single-stranded DNA and interacts with multiple proteins, including
Eugenia Santos et al.
The Journal of biological chemistry, 289(5), 2888-2898 (2013-12-11)
The LEXE motif, conserved in eukaryotic type DNA polymerases, is placed close to the polymerization active site. Previous studies suggested that the second Glu was involved in binding a third noncatalytic ion in bacteriophage RB69 DNA polymerase. In the protein-primed
Bentley M Wingert et al.
Biochemistry, 52(44), 7723-7730 (2013-10-24)
Plasmodium falciparum, a parasitic organism and one of the causative agents of malaria, contains an unusual organelle called the apicoplast. The apicoplast is a nonphotosynthetic plastid responsible for supplying the parasite with isoprenoid units and is therefore indispensable. Like mitochondria
Bin Zhou et al.
Journal of virology, 88(19), 11121-11129 (2014-07-11)
Previous reports showed that raltegravir, a recently approved antiviral compound that targets HIV integrase, can inhibit the nuclease function of human cytomegalovirus (HCMV terminase) in vitro. In this study, subtoxic levels of raltegravir were shown to inhibit the replication of
Yongyun Zhao et al.
Nature communications, 4, 1493-1493 (2013-02-21)
RNA detection has become an integral part of current biomedical research. Up to now, the reverse transcription-PCR has been the most practical method to detect mRNA targets. However, RNA detection by reverse transcription-PCR requires sophisticated equipment and it is highly
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