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Francesca De Faveri et al.
Autophagy, 16(7), 1314-1331 (2019-10-28)
Activation of trypsinogen (formation of trypsin) inside the pancreas is an early pathological event in the development of acute pancreatitis. In our previous studies we identified the activation of trypsinogen within endocytic vacuoles (EVs), cellular organelles that appear in pancreatic
Use of digitonin-permeabilized adipocytes for cAMP studies.
R A Mooney
Methods in enzymology, 159, 193-202 (1988-01-01)
Christian Menge et al.
Innate immunity, 21(6), 655-664 (2015-04-25)
Shiga toxin (Stx)-producing Escherichia coli (STEC) infections in cattle are asymptomatic; however, Stx impairs the initiation of an adaptive immune response by targeting bovine peripheral and intraepithelial lymphocytes. As presumptive bovine mucosal macrophages (Mø) are also sensitive to Stx, STEC
Ramona Madalina Babes et al.
Cell cycle (Georgetown, Tex.), 17(6), 766-779 (2018-02-09)
Human leukemia Jurkat T cells were analyzed for apoptosis and cell cycle by flow cytometry, using the Annexin V/propidium iodide (PI) standard assay, and a simple PI staining in Triton X-100/digitonin-enriched PI/RNase buffer, respectively. Cells treated with doxorubicin or menadione
Sreekanth Rajan et al.
Scientific reports, 5, 10609-10609 (2015-05-30)
Bcl-2 family proteins are key regulators for cellular homeostasis in response to apoptotic stimuli. Bcl-xL, an antiapoptotic Bcl-2 family member, undergoes conformational transitions, which leads to two conformational states: the cytoplasmic and membrane-bound. Here we present the crystal and small-angle
Synergistic interactions of saponins and monoterpenes in HeLa cells, Cos7 cells and in erythrocytes.
Florian Herrmann et al.
Phytomedicine : international journal of phytotherapy and phytopharmacology, 18(13), 1191-1196 (2011-10-05)
In phytomedicine complex extracts consisting of phenolics, monoterpenes or saponins are traditionally used. It is often impossible to attribute the biological activity of an extract to one or few compounds. As an explanation of the superior activity of extracts, a
Michael L van de Weijer et al.
Nature communications, 5, 3832-3832 (2014-05-09)
Misfolded ER proteins are retrotranslocated into the cytosol for degradation via the ubiquitin-proteasome system. The human cytomegalovirus protein US11 exploits this ER-associated protein degradation (ERAD) pathway to downregulate HLA class I molecules in virus-infected cells, thereby evading elimination by cytotoxic
Sankar Bhattacharyya et al.
The Journal of general virology, 95(Pt 1), 71-79 (2013-10-12)
Japanese encephalitis virus (JEV) infection-induced encephalitis causes extensive death or long-term neurological damage, especially among children, in south and south-east Asia. Infection of mammalian cells has shown induction of an unfolded protein response (UPR), presumably leading to programmed cell death
A B Kotliarova et al.
Ukrains'kyi biokhimichnyi zhurnal (1999 ), 85(5), 27-36 (2014-02-01)
The role of inositol-1,4,5-trisphosphate of (IP3)-sensitive Ca2+ channels in Ca2+ homeostasis maintenance under activation of M-cholinergic receptors and P2Y receptors in the secretory cells of the rat lacrimal gland was investigated. The study was carried out on intact and permeabilized
Hugo Botebol et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(47), 14652-14657 (2015-11-11)
In large regions of the open ocean, iron is a limiting resource for phytoplankton. The reduction of iron quota and the recycling of internal iron pools are among the diverse strategies that phytoplankton have evolved to allow them to grow
James Hamilton et al.
Human molecular genetics, 24(17), 4862-4878 (2015-06-05)
Alterations in oxidative metabolism are considered to be one of the major contributors to Huntington's disease (HD) pathogenesis. However, existing data about oxidative metabolism in HD are contradictory. Here, we investigated the effect of mutant huntingtin (mHtt) on oxidative metabolism
J Liu et al.
Methods (San Diego, Calif.), 19(3), 403-409 (1999-12-02)
The application of a cell permeabilization technique to the analysis of nuclear import has led to many major breakthroughs in our understanding of this trafficking pathway. Digitonin permeabilization maintains the nucleus in a state competent for faithful, signal-dependent translocation through
Chongshu Jian et al.
Cell metabolism, 31(5), 892-908 (2020-05-07)
Nonalcoholic steatohepatitis (NASH) is becoming one of the leading causes of hepatocellular carcinoma (HCC). Sorafenib is the only first-line therapy for advanced HCC despite its serious adverse effects. Here, we report that at an equivalent of approximately one-tenth the clinical
Brian C Evans et al.
ACS nano, 9(6), 5893-5907 (2015-05-26)
A platform technology has been developed and tested for delivery of intracellular-acting peptides through electrostatically complexed nanoparticles, or nano-polyplexes, formulated from an anionic endosomolytic polymer and cationic therapeutic peptides. This delivery platform has been initially tested and optimized for delivery
Carola Stockburger et al.
Journal of Alzheimer's disease : JAD, 42(2), 395-411 (2014-06-06)
Recent data suggest that the combined effect of oxidative stress due to aging and slightly elevated amyloid-β (Aβ) levels initiate Alzheimer's disease (AD) long before the clinical onset. Investigations of this early phase are hampered by the lack of cellular
Aurélia Cassany et al.
Journal of virology, 89(3), 1719-1730 (2014-11-21)
In this study, we characterized the molecular basis for binding of adenovirus (AdV) to the cytoplasmic face of the nuclear pore complex (NPC), a key step during delivery of the viral genome into the nucleus. We used RNA interference (RNAi)
Xiaojie Wu et al.
The Analyst, 139(16), 4056-4063 (2014-06-20)
Neutrophils are critical inflammatory cells; thus, it is important to characterize the effects of drugs on neutrophil function in the context of inflammatory diseases. Herein, chemically guided neutrophil migration, known as chemotaxis, is studied in the context of drug treatment
Claudio A Pereira et al.
Experimental parasitology, 142, 43-50 (2014-04-29)
Nucleoside diphosphate kinase (NDPK) is a key enzyme in the control of cellular concentrations of nucleoside triphosphates, and has been shown to play important roles in many cellular processes. In this work we investigated the subcellular localization of the canonical
Masayuki Saito et al.
Bioscience, biotechnology, and biochemistry, 76(9), 1777-1780 (2012-09-14)
The visualization of nuclear architecture, which changes dynamically depending on the physiological and the pathological situation, remains an important challenge. Here we report that exposure of fluoresceinisothiocyanate-labeled polyethylenimine (FITC-PEI) to digitonin-permeabilized cervical cancer HeLa cells enable rapid detection of the
Chou-Wei Chang et al.
Journal of virology, 89(3), 1703-1718 (2014-11-21)
BGLF4 kinase, the only Ser/Thr protein kinase encoded by the Epstein-Barr virus (EBV) genome, phosphorylates multiple viral and cellular substrates to optimize the cellular environment for viral DNA replication and the nuclear egress of nucleocapsids. Previously, we found that nuclear
Elin Rönnberg et al.
International archives of allergy and immunology, 165(1), 68-74 (2014-10-25)
Many of the functions attributed to mast cells depend on the various pro-inflammatory mediators that are secreted upon mast cell activation. These include a panel of mast cell-specific proteases. In addition, recent studies have indicated that murine mast cells also
Grainne S Gorman et al.
Clinical science (London, England : 1979), 128(12), 895-904 (2015-01-30)
Complex I (CI) is the largest of the five multi-subunit complexes constituting the human oxidative phosphorylation (OXPHOS) system. Seven of its catalytic core subunits are encoded by mitochondrial DNA (ND (NADH dehydrogenase)1-6, ND4L (NADH dehydrogenase subunit 4L)), with mutations in
Adrian Flierl et al.
PloS one, 9(11), e112413-e112413 (2014-11-13)
Parkinson disease (PD) is a multi-factorial neurodegenerative disorder with loss of dopaminergic neurons in the substantia nigra and characteristic intracellular inclusions, called Lewy bodies. Genetic predisposition, such as point mutations and copy number variants of the SNCA gene locus can
Nunnapas Jiwlawat et al.
Biotechnology and bioengineering, 116(9), 2377-2392 (2019-05-28)
Recent advances in bioengineering have enabled cell culture systems that more closely mimic the native cellular environment. Here, we demonstrated that human induced pluripotent stem cell (iPSC)-derived myogenic progenitors formed highly-aligned myotubes and contracted when seeded on two-dimensional micropatterned platforms.
Eri Takeda et al.
PloS one, 6(11), e27815-e27815 (2011-11-24)
Vpr, an accessory protein of human immunodeficiency virus type 1, is a multifunctional protein that plays an important role in viral replication. We have previously shown that the region between residues 17 and 74 of Vpr (Vpr(N17C74)) contained a bona
Ying Qiu et al.
PloS one, 9(12), e114371-e114371 (2014-12-09)
The ATP-gated P2X7 has been shown to play an important role in invasiveness and metastasis of some tumors. However, the possible links and underlying mechanisms between P2X7 and prostate cancer have not been elucidated. Here, we demonstrated that P2X7 was
Kirsteen M Tullett et al.
eLife, 9 (2020-12-03)
The dendritic cell receptor Clec9A facilitates processing of dead cell-derived antigens for cross-presentation and the induction of effective CD8+ T cell immune responses. Here, we show that this process is regulated by E3 ubiquitin ligase RNF41 and define a new
Bettina Hüntelmann et al.
PloS one, 9(5), e97633-e97633 (2014-05-23)
Binding to specific palindromic sequences termed gamma-activated sites (GAS) is a hallmark of gene activation by members of the STAT (signal transducer and activator of transcription) family of cytokine-inducible transcription factors. However, the precise molecular mechanisms involved in the signal-dependent
Ying-Nai Wang et al.
The Journal of biological chemistry, 287(20), 16869-16879 (2012-03-28)
Nuclear localization of multiple receptor-tyrosine kinases (RTKs), such as EGF receptor (EGFR), ErbB-2, FGF receptor (FGFR), and many others, has been reported by several groups. We previously showed that cell surface EGFR is trafficked to the nucleus through a retrograde
Yael Hacohen et al.
Neurology, 85(11), 967-975 (2015-08-25)
To assess the clinical and immunologic findings in children with voltage-gated potassium channel (VGKC)-complex antibodies (Abs). Thirty-nine of 363 sera, referred from 2 pediatric centers from 2007 to 2013, had been reported positive (.100 pM) for VGKC-complex Abs. Medical records
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