D5758

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Comparative Analysis of Denitrifying Activities of Hyphomicrobium nitrativorans, Hyphomicrobium denitrificans, and Hyphomicrobium zavarzinii.

Christine Martineau et al.

Applied and environmental microbiology, 81(15), 5003-5014 (2015-05-17)

Hyphomicrobium spp. are commonly identified as major players in denitrification systems supplied with methanol as a carbon source. However, denitrifying Hyphomicrobium species are poorly characterized, and very few studies have provided information on the genetic and physiological aspects of denitrification

Poly-adenine-based programmable engineering of gold nanoparticles for highly regulated spherical DNAzymes.

Dan Zhu et al.

Nanoscale, 7(44), 18671-18676 (2015-10-27)

Enzyme complexes are assembled at the two-dimensional lipid membrane or prearranged on three-dimensional scaffolding proteins to regulate their catalytic activity in cells. Inspired by nature, we have developed gold nanoparticle-based spherical DNAzymes (SNAzymes) with programmably engineered activities by exploiting poly-adenine

One octarepeate expansion to the human prion protein alters both the Zn2+ and Cu2+ coordination environments within the octarepeate domain.

Jason Shearer et al.

Inorganic chemistry, 50(4), 1173-1175 (2011-01-22)

The influence of a single octarepeat expansion on the Cu(II) and Zn(II) coordination environments within the octarepeat domain of the human prion protein is examined. Using X-ray absorption spectroscopy and diethyl pyrocarbonate labeling studies, we find that at low copper

NrdI essentiality for class Ib ribonucleotide reduction in Streptococcus pyogenes.

Ignasi Roca et al.

Journal of bacteriology, 190(14), 4849-4858 (2008-05-27)

The Streptococcus pyogenes genome harbors two clusters of class Ib ribonucleotide reductase genes, nrdHEF and nrdF*I*E*, and a second stand-alone nrdI gene, designated nrdI2. We show that both clusters are expressed simultaneously as two independent operons. The NrdEF enzyme is

Sox2 function as a negative regulator to control HAMP expression.

Bin Song et al.

Biological research, 48, 23-23 (2015-05-07)

Hepcidin, encoding by HAMP gene, is the pivotal regulator of iron metabolism, controlling the systemic absorption and transportation of irons from intracellular stores. Abnormal levels of HAMP expression alter plasma iron parameters and lead to iron metabolism disorders. Therefore, it

Construction of RNA-Quantum Dot Chimera for Nanoscale Resistive Biomemory Application.

Taek Lee et al.

ACS nano, 9(7), 6675-6682 (2015-07-03)

RNA nanotechnology offers advantages to construct thermally and chemically stable nanoparticles with well-defined shape and structure. Here we report the development of an RNA-QD (quantum dot) chimera for resistive biomolecular memory application. Each QD holds two copies of the pRNA

MicroRNA signatures of platinum-resistance in ovarian cancer.

H C Ying et al.

European journal of gynaecological oncology, 36(1), 16-20 (2015-04-16)

The authors utilized a microRNA (miRNA) array to compare the differentially expressed miRNAs in platinum-resistant associated ovarian cancer cells. The differential expression of microRNA between COC1 (DDP-sensitive) and platinum-resistant COC1/DDP (DDP-resistant) tumor cell lines was determined using microarray. Expression levels

Pi2 spacer pyrocarbon arthroplasty technique for thumb basal joint osteoarthritis.

Philippe Bellemère et al.

Techniques in hand & upper extremity surgery, 15(4), 247-252 (2011-11-23)

Trapeziectomy associated with a pyrocarbon implant is a recent procedure for thumb basal joint osteoarthritis. The investigators report Pi pyrocarbon implant technique (Tornier Bioprofile, Grenoble, France) for primary thumb basal joint osteoarthritis. The key points of the procedure are preservation

Moderate-intensity exercise training elevates serum and pancreatic zinc levels and pancreatic ZnT8 expression in streptozotocin-induced diabetic rats.

Juraiporn Somboonwong et al.

Life sciences, 139, 46-51 (2015-08-19)

Diabetes mellitus is associated with disturbed zinc homeostasis and down-regulation of zinc transporter 8 (ZnT8); these changes contribute to the defective biosynthesis, storage, and secretion of insulin. Previous studies have reported an improvement in diabetic status and insulin levels in

Increased protein structural resolution from diethylpyrocarbonate-based covalent labeling and mass spectrometric detection.

Yuping Zhou et al.

Journal of the American Society for Mass Spectrometry, 23(4), 708-717 (2012-02-03)

Covalent labeling and mass spectrometry are seeing increased use together as a way to obtain insight into the 3-dimensional structure of proteins and protein complexes. Several amino acid specific (e.g., diethylpyrocarbonate) and non-specific (e.g., hydroxyl radicals) labeling reagents are available

Gene expression in mdx mouse muscle in relation to age and exercise: aberrant mechanical-metabolic coupling and implications for pre-clinical studies in Duchenne muscular dystrophy.

Giulia Maria Camerino et al.

Human molecular genetics, 23(21), 5720-5732 (2014-06-12)

Weakness and fatigability are typical features of Duchenne muscular dystrophy patients and are aggravated in dystrophic mdx mice by chronic treadmill exercise. Mechanical activity modulates gene expression and muscle plasticity. Here, we investigated the outcome of 4 (T4, 8 weeks

Genome-, Transcriptome- and Proteome-Wide Analyses of the Gliadin Gene Families in Triticum urartu.

Yanlin Zhang et al.

PloS one, 10(7), e0131559-e0131559 (2015-07-02)

Gliadins are the major components of storage proteins in wheat grains, and they play an essential role in the dough extensibility and nutritional quality of flour. Because of the large number of the gliadin family members, the high level of

Particulate matter phagocytosis induces tissue factor in differentiating macrophages.

M Milano et al.

Journal of applied toxicology : JAT, 36(1), 151-160 (2015-04-11)

Airborne exposure to particulate matter with diameter < 10 mcM (PM10) has been linked to an increased risk of thromboembolic events, but the mechanisms are not completely understood. The aim of this study was to evaluate the effect of PM10 phagocytosis on

Protective effect of Xin Mai Jia ultrafiltration extract on human umbilical vein endothelial cell injury induced by hydrogen peroxide and the effect on the NO-cGMP signaling pathway.

Yaling Yin et al.

Experimental and therapeutic medicine, 8(1), 38-48 (2014-06-20)

The aim of the present study was to evaluate the protective effect of the ultrafiltration extract of Xin Mai Jia (XMJ) on a human umbilical vein endothelial cell (HUVEC) injury model induced by hydrogen peroxide (H

Nuclear Protein Sam68 Interacts with the Enterovirus 71 Internal Ribosome Entry Site and Positively Regulates Viral Protein Translation.

Hua Zhang et al.

Journal of virology, 89(19), 10031-10043 (2015-07-24)

Enterovirus 71 (EV71) recruits various cellular factors to assist in the replication and translation of its genome. Identification of the host factors involved in the EV71 life cycle not only will enable a better understanding of the infection mechanism but

Suramin inhibits cell proliferation in ovarian and cervical cancer by downregulating heparanase expression.

HuaPing Li et al.

Cancer cell international, 15, 52-52 (2015-06-09)

Aberrant expression of heparanase (Hpa) is associated with apoor prognosis in ovarian and cervical cancer patients. Inhibitors of Hpa can prevent the growth and metastasis of malignant tumor cells, and suramin may be such a compound that has strong anti-proliferative

Diethylpyrocarbonate labeling for the structural analysis of proteins: label scrambling in solution and how to avoid it.

Yuping Zhou et al.

Journal of the American Society for Mass Spectrometry, 23(5), 899-907 (2012-02-22)

Covalent labeling along with mass spectrometry is a method that is increasingly used to study protein structure. Recently, it has been shown that diethylpyrocarbonate (DEPC) is a powerful labeling reagent because it can modify up to 30% of the residues

Preparation and Characterization of Dictyophora indusiata Polysaccharide-Zinc Complex and Its Augmented Antiproliferative Activity on Human Cancer Cells.

Wenzhen Liao et al.

Journal of agricultural and food chemistry, 63(29), 6525-6534 (2015-07-15)

Dictyophora indusiata polysaccharide (DP1) was successfully chelated with zinc chloride to achieve its enhanced antiproliferative activity. The obtained DP1-Zn complex showed significant antiproliferative activity (18.1 ± 2.84% viability of MCF-7 cells at 250 μg/mL) on a group of human cancer

[Mid-term outcome after implantation of a pyrocarbon endoprosthesis in patients with degenerative arthritis].

G Heers et al.

Zeitschrift fur Orthopadie und Unfallchirurgie, 150(3), 324-328 (2012-04-21)

The aim of this study was to make a mid-term evaluation of an unconstrained pyrocarbon prosthesis (Ascension®) in the treatment of idiopathic degenerative arthritis of the proximal interphalangeal joint of the hand. 13 implants (10 patients) were clinically and radiologically

Novel plasticity rule can explain the development of sensorimotor intelligence.

Ralf Der et al.

Proceedings of the National Academy of Sciences of the United States of America, 112(45), E6224-E6232 (2015-10-28)

Grounding autonomous behavior in the nervous system is a fundamental challenge for neuroscience. In particular, self-organized behavioral development provides more questions than answers. Are there special functional units for curiosity, motivation, and creativity? This paper argues that these features can

Photolithographic Synthesis of High-Density DNA and RNA Arrays on Flexible, Transparent, and Easily Subdivided Plastic Substrates.

Matthew T Holden et al.

Analytical chemistry, 87(22), 11420-11428 (2015-10-24)

The photolithographic fabrication of high-density DNA and RNA arrays on flexible and transparent plastic substrates is reported. The substrates are thin sheets of poly(ethylene terephthalate) (PET) coated with cross-linked polymer multilayers that present hydroxyl groups suitable for conventional phosphoramidite-based nucleic

Growth of cedar-like Au nanoparticles coating on an etched stainless steel wire and its application for selective solid-phase microextraction.

Yida Zhang et al.

Analytica chimica acta, 876, 55-62 (2015-05-23)

A novel cedar-like Au nanoparticles (AuNPs) coating was fabricated on an etched stainless steel (SS) wire by direct chemical deposition and used as an efficient and unbreakable solid phase microextraction (SPME) fiber. The etched SS wire offers a rough surface

Ribonucleotide reductase NrdR as a novel regulator for motility and chemotaxis during adherent-invasive Escherichia coli infection.

Nicolas Dreux et al.

Infection and immunity, 83(4), 1305-1317 (2015-01-22)

A critical step in the life cycle of all organisms is the duplication of the genetic material during cell division. Ribonucleotide reductases (RNRs) are essential enzymes for this step because they control the de novo production of the deoxyribonucleotides required

Substrate-induced conformational changes in sarcoplasmic reticulum Ca2+-ATPase probed by surface modification using diethylpyrocarbonate with mass spectrometry.

Ryouhei Narumi et al.

FEBS letters, 586(19), 3172-3178 (2012-07-10)

We have identified 15 residues from the surface of sarcoplasmic reticulum Ca(2+)-pump ATPase, by mass spectrometry using diethylpyrocarbonate modification. The reactivity of 9 residues remained high under all the conditions. The reactivity of Lys-515 at the nucleotide site was severely

Germline transgenesis in rodents by pronuclear microinjection of Sleeping Beauty transposons.

Zoltán Ivics et al.

Nature protocols, 9(4), 773-793 (2014-03-15)

We describe a protocol for high-efficiency germline transgenesis and sustained transgene expression in two important biomedical models, the mouse and the rat, by using the Sleeping Beauty transposon system. The procedure is based on co-injection of synthetic mRNA encoding the

Multi-electrode array capable of supporting precisely patterned hippocampal neuronal networks.

Tianyi Zhou et al.

Biomedical microdevices, 17(1), 2-2 (2015-02-06)

Accurate positioning of primary mouse hippocampal neurons on electrodes enables the recording from and stimulation of specified individual neurons on a multi-electrode array (MEA). In this work, positive dielectrophoresis (DEP) is applied to actively recruit hippocampal neurons to the electrodes

[Proximal row carpectomy and replacement of the proximal pole of the capitate by means of a pyrocarbon cap (RCPI) in advanced carpal collapse].

G Szalay et al.

Handchirurgie, Mikrochirurgie, plastische Chirurgie : Organ der Deutschsprachigen Arbeitsgemeinschaft fur Handchirurgie : Organ der Deutschsprachigen Arbeitsgemeinschaft fur Mikrochirurgie der Peripheren Nerven und Gefasse : Organ der V..., 44(1), 17-22 (2011-08-27)

The results of 5 patients suffering from advanced carpal collapse after proximal row carpectomy and replacement of the proximal pole of the capitate by means of a pyrocarbon cap are presented.5 patients with an average age of 40.2 years (23-66

Tumor Necrosis Factor and Schistosoma mansoni egg antigen omega-1 shape distinct aspects of the early egg-induced granulomatous response.

Kevin K Takaki et al.

PLoS neglected tropical diseases, 15(1), e0008814-e0008814 (2021-01-20)

Infections by schistosomes result in granulomatous lesions around parasite eggs entrapped within the host tissues. The host and parasite determinants of the Schistosoma mansoni egg-induced granulomatous response are areas of active investigation. Some studies in mice implicate Tumor Necrosis Factor

Curcumin inhibits PhIP induced cytotoxicity in breast epithelial cells through multiple molecular targets.

Ashok Jain et al.

Cancer letters, 365(1), 122-131 (2015-05-26)

Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), found in cooked meat, is a known food carcinogen that causes several types of cancer, including breast cancer, as PhIP metabolites produce DNA adduct and DNA strand breaks. Curcumin, obtained from the rhizome of Curcuma longa, has potent

Purification and Use of tRNA for Enzymatic Post-translational Addition of Amino Acids to Proteins.

Irem Avcilar-Kucukgoze et al.

STAR protocols, 1(3), 100207-100207 (2020-12-31)

Post-translational addition of amino acids to proteins by enzymes using aminoacyl-tRNA is an emerging regulatory mechanism. Examples include Arg transfer in eukaryotes, Leu/Phe transfer in bacteria, and tRNA-synthetase-mediated addition of amino acids to Lys side chains. Here, we present a

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