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Matej Adámik et al.
Biochemical and biophysical research communications, 456(1), 29-34 (2014-12-03)
Site-specific DNA recognition and binding activity belong to common attributes of all three members of tumor suppressor p53 family proteins: p53, p63 and p73. It was previously shown that heavy metals can affect p53 conformation, sequence-specific binding and suppress p53
Lili Ma et al.
Molecular medicine reports, 12(2), 2529-2538 (2015-05-09)
Premature ovarian failure (POF) is an ovarian defect characterized by the premature depletion of ovarian follicles in individuals <40 years old, and is a major cause of infertility in females. Genetic factors are considered to be responsible for the development
Mohamad Zandian et al.
STAR protocols, 3(4), 101853-101853 (2023-01-04)
The SARS-CoV-2 envelope (E) protein hijacks human BRD4 (bromodomain and extra-terminal domain protein 4). Here, we describe a protocol to characterize the interaction of the acetylated E protein with BRD4 in vivo. We detail steps to use NMR spectroscopy to map the
EDTA: the chelating agent under environmental scrutiny
Oviedo C and Rodriguez J
Quimica nova, 26(6), 901-905 (2003)
Mingqiang Li et al.
Biomaterials, 51, 161-172 (2015-03-17)
As a synergistic drug combination, doxorubicin-loaded cisplatin crosslinked polysaccharide-based nanoparticles (Dex-SA-DOX-CDDP) have demonstrated enhanced antitumor efficacy and reduced systemic toxicity via optimized biodistribution, controlled drug release, prolonged blood circulation, and improved tolerability, compared to the non-crosslinked nanoparticles or free doxorubicin.
Ruibing Chen et al.
Journal of proteome research, 13(12), 5767-5776 (2014-09-13)
Temperature changes influence the reaction rates of all biological processes, which can pose dramatic challenges to cold-blooded organisms, and the capability to adapt to temperature fluctuations is crucial for the survival of these animals. In order to understand the roles
R Odore et al.
Poultry science, 94(8), 1979-1985 (2015-05-28)
Tetracyclines, which represent one of the most commonly used antibiotics for poultry, are known to be deposited in bones, where they can remain, despite the observation of appropriate withdrawal times. The aim of the study was to determine the concentration
Tomoyuki Tanaka et al.
Nature protocols, 5(1), 67-92 (2010-01-09)
Single-domain intracellular antibodies are antibody variable segments that bind to specific target proteins inside cells. These antigen-binding variable regions can interfere with protein function or perturb protein-protein interactions and can be used as tools for research, especially functional genomics and
Catherine W Macharia-Mutie et al.
Public health nutrition, 16(9), 1605-1613 (2012-12-12)
Simulating the probable impact of grain amaranth and highly absorbable, low-Fe micronutrient powder (MNP) on Fe status in a potential target population is an essential step in choosing and developing an appropriate actual intervention. We simulated the potential effect of
Fast ultracentrifugation methods for the separation of plasma lipoproteins.
J M Ordovas
Methods in molecular biology (Clifton, N.J.), 110, 93-103 (1999-01-26)
Determination of vanadium in groundwater samples with an improved kinetic spectrophotometric method.
Esra Bağda
Environmental technology, 35(9-12), 1165-1174 (2014-04-08)
A kinetic catalytic method has been developed for the determination of vanadium based on its catalytic effect on the redox reaction of azorubin S and bromate in the presence of a sulphuric and nitric acid mixture. The reaction is monitored
Jose Manuel Vagace et al.
Journal of clinical pathology, 66(9), 811-814 (2013-06-01)
We investigated the case of a 14-year-old girl with an ethylenediaminetetraacetic acid (EDTA)-dependent haemagglutination detected by macrocytosis, which was only evident by an abnormal red blood cell (RBC) population in the histogram. Investigations included haemograms with different anticoagulants and experimental
Heesun Kim et al.
eLife, 10 (2021-05-19)
Eukaryotic cells use guided search to coordinately control dispersed genetic elements. Argonaute proteins and their small RNA cofactors engage nascent RNAs and chromatin-associated proteins to direct transcriptional silencing. The small ubiquitin-like modifier (SUMO) has been shown to promote the formation
Theodore A Craig et al.
Biochemical and biophysical research communications, 433(4), 508-512 (2013-03-26)
The EF-hand protein, DREAM/KChIP3 (henceforth referred to as DREAM), regulates apoptosis by incompletely understood mechanisms. We demonstrate that in the presence of Ca2+, DREAM interacts with hexokinase I, a protein known to bind mitochondria and regulate apoptosis. A mutant DREAM
Carolina Kato Prado et al.
Food additives & contaminants. Part B, Surveillance, 8(2), 81-84 (2014-09-24)
The aim of this study was to investigate the presence of tetracycline residues in pasteurised cow's milk using high-performance liquid chromatography coupled with UV/VIS detection to determine the exposure of Brazilian's population to antibiotic residues. One hundred samples collected from
Iman Tahmasbian et al.
Environmental monitoring and assessment, 185(11), 8847-8860 (2013-05-21)
The application of electrical fields and chelating agents is an innovative hybrid technology used for the decontamination of soil polluted by heavy metals. The effects of four center-oriented electrical fields and chelating agents on active fractions of lead and zinc
Naoko Ohashi et al.
The American journal of emergency medicine, 31(7), 1157-1157 (2013-05-25)
EDTA-dependent pseudothrombocytopenia (EDTA-PTCP) is a phenomenon that occurs in vitro when EDTA reacts with harvested blood. EDTA-dependent pseudothrombocytopenia usually does not indicate thrombocytopenia in vivo. Here, we report the first case of EDTA-PTCP complicated by eosinophilic pneumonia. A 70-year-old man
Adaris M López Marzo et al.
Biosensors & bioelectronics, 47, 190-198 (2013-04-13)
In this work for first time a lateral flow immunosensor device (LFID) for Cd(2+) determination in drinking and tap waters using the Cd-EDTA-BSA-AuNP conjugate as signal producer tool is introduced. The principle of working is based on competitive reaction between
Alanah L Bradey et al.
Scientific reports, 12(1), 13128-13128 (2022-07-31)
Multiple myeloma (MM) is an incurable haematological malignancy, caused by the uncontrolled proliferation of plasma cells within the bone marrow (BM). Obesity is a known risk factor for MM, however, few studies have investigated the potential of dietary intervention to
Panagiotis G Xenoulis et al.
BMC veterinary research, 9, 47-47 (2013-03-19)
Despite the importance of abnormalities in lipoprotein metabolism in clinical canine medicine, the fact that most previously used methods for lipoprotein profiling are rather laborious and time-consuming has been a major obstacle to the wide clinical application and use of
Arup Acharjee et al.
Journal of experimental zoology. Part A, Ecological genetics and physiology, 323(8), 567-585 (2015-07-25)
Gonadotropins are heterodimeric glycoproteins secreted by the pituitary, and consist of a common glycoprotein hormone alpha (GPα) and the function-specific follicle-stimulating hormone beta subunit (FSHβ) or luteinizing hormone beta subunit (LHβ). In the present study, the subunit protein genes were
Quan Zhang et al.
Environmental science & technology, 48(12), 6995-7001 (2014-05-23)
As the substitute of polybrominated diphenyl ethers (PBDEs), further assessments about the potential ecological safety and health risks of phosphorus-containing flame retardants (PFRs) are required because the worldwide demand for PFRs has been increasing every year. In this study, we
Gervasio A Lamas et al.
American heart journal, 168(1), 37-44 (2014-06-24)
Disodium ethylenediaminetetraacetic acid (EDTA) reduced adverse cardiac outcomes in a factorial trial also testing oral vitamins. This report describes the intent-to-treat comparison of the 4 factorial groups overall and in patients with diabetes. This was a double-blind, placebo-controlled, 2 ×
Luminita David et al.
Colloids and surfaces. B, Biointerfaces, 122, 767-777 (2014-09-02)
This research aimed at reporting the synthesis, characterization and evaluation of the anti-inflammatory effects of some new biomaterials based on silver nanoparticles and polyphenols rich natural extracts. A fast and eco-friendly extracellular biosynthesis of silver nanoparticles (AgNPs), using European black
Salah Al Zadjali et al.
International journal of clinical and experimental medicine, 8(5), 7160-7167 (2015-07-30)
Lead is a widely-spread environmental pollutant and a commonly-used industrial chemical that can cause multisystemic adverse health effects. However, the effects of lead exposure on diabetic animals have not been reported so far. The aim of this study is to
Ilya B Zavodnik et al.
Life sciences, 92(23), 1110-1117 (2013-05-07)
The present study was designed for further evaluation of the biochemical mechanism of hepatic mitochondrial dysfunction under oxidative damages induced by organic hydroperoxide, tert-butyl hydroperoxide (tBHP), for estimation of the molecular targets impaired during oxidative stress, and for investigation of
[Alternative medicine sensation. Surprisingly effective: chelates and multivitamins in coronary heart disease].
Dirk Einecke
MMW Fortschritte der Medizin, 155(6), 23-23 (2013-05-25)
Yi-Tsang Lee et al.
Methods in enzymology, 654, 255-270 (2021-06-15)
Optogenetics combines optics and genetics to enable non-invasive interrogation of cell physiology at an unprecedented high spatiotemporal resolution. Here, we introduce Opto-CRAC as a set of genetically-encoded calcium actuators (GECAs) engineered from the calcium release-activated calcium (CRAC) channel, which has
S N Kozlov et al.
Zhurnal mikrobiologii, epidemiologii, i immunobiologii, (2)(2), 3-12 (2013-06-29)
Detection ofproteases in outer membranes (OM) of ompT+ and ompT- Vibrio cholerae strains of O1 and O139 serogroups. Specific sterile preparations of OM were obtained by lysis of live V. cholerae cells by 4.5 M urea solution with subsequent differential
A A Romani et al.
Journal of peptide science : an official publication of the European Peptide Society, 19(9), 554-565 (2013-07-31)
Antimicrobial-peptide-based therapies could represent a reliable alternative to overcome antibiotic resistance, as they offer potential advantages such as rapid microbicidal activity and multiple activities against a broad spectrum of bacterial pathogens. Three synthetic antimicrobial peptides (AMPs), AMP72, AMP126, and also
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