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Mary W Trucksess et al.
Journal of AOAC International, 91(3), 511-523 (2008-06-24)
The accuracy, repeatability, and reproducibility characteristics of a method using multitoxin immunoaffinity column cleanup with liquid chromatography (LC) for determination of aflatoxins (AF; sum of aflatoxins B1, B2, G1, and G2) and ochratoxin A (OTA) in powdered ginseng and ginger
Zheng Han et al.
Analytica chimica acta, 664(2), 165-171 (2010-04-07)
An ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for simultaneous determination of aflatoxins B1, B2, G1, G2, M1 and M2 in traditional Chinese medicines (TCMs) was developed. The approach was characterized in details and a special focus was placed on
E V Siloto et al.
Poultry science, 92(8), 2077-2083 (2013-07-23)
Aflatoxins (AF) and fumonisins (FU) are a major problem faced by poultry farmers, leading to huge economic losses. This experiment was conducted to determine the effects of AF (1 mg/kg of feed) and FU (25 mg/kg of feed), singly or
Gonzalo J Diaz et al.
Journal of AOAC International, 95(4), 1084-1088 (2012-09-14)
The stability of aflatoxins B1, B2, G1, and G2 was studied in solutions containing different concentrations of water, acetonitrile, and/or methanol, and in autosampler vials treated with nitric acid or silanized. When stored at room temperature (20 degrees C) for
Zarrin Es'haghi et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(28), 3034-3040 (2011-09-20)
The new pre-concentration technique, hollow fiber-solid phase microextraction based on carbon nanotube reinforced sol-gel and liquid chromatography-photodiode array detection was applied to determination of aflatoxins B(1), B(2) (AFB(1), AFB(2)) in rice, peanut and wheat samples. This research provides an overview
G Bianco et al.
Toxicon : official journal of the International Society on Toxinology, 59(6), 644-650 (2012-03-10)
Aflatoxins are natural contaminants frequently found both in food and feed. Many of them exert immunomodulatory properties in mammals; therefore, the aim of the current study was to investigate immune-effects of AFB1, AFB2, AFM1 and AFM2, alone and differently combined
Si Chen et al.
Analytical and bioanalytical chemistry, 405(5), 1623-1630 (2013-01-15)
This article describes the use of microwave-assisted extraction (MAE) as a pretreatment technique for the determination of aflatoxins B(1), G(1), B(2), and G(2) in grains and grain products. The optimal operation parameters, including extraction solvent, temperature, and time, were identified
Emmanuel Njumbe Ediage et al.
Food chemistry, 177, 397-404 (2015-02-11)
An LC-MS/MS method was developed and validated for the detection and quantification of 23 mycotoxins in different varieties of sorghum. The method performance characteristics were as follows: suitable linearity ranges for all 23 mycotoxins with p-value >0.05; limits of detection
Lei Bao et al.
Journal of AOAC International, 96(5), 1017-1018 (2013-11-29)
A collaborative study of a method for determination of aflatoxins (AFs) B1, B2, G1, and G2 in olive oil, peanut oil, and sesame oil using immunoaffinity column cleanup, postcolumn derivatization, and LC with fluorescence detection, previously published in J. AOAC
Shen Han et al.
Se pu = Chinese journal of chromatography, 29(7), 613-617 (2011-11-22)
A method for the determination of five aflatoxins (B1 , B2, G1 , G2, M1 ) in Chinese patent medicines and medicinal herbs by immunoaffinity extraction coupled with ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was developed. The samples were
Xiaomeng Wu et al.
The Analyst, 137(18), 4226-4234 (2012-07-27)
High-quality surface-enhanced Raman scattering (SERS) spectra of aflatoxin (AF) B(1), B(2), G(1) and G(2) have been acquired using silver nanorod (AgNR) array substrates fabricated by oblique angle deposition method. Significant vibrational peaks are identified on the argon plasma-cleaned substrates, and
Debopam Acharya et al.
Analytica chimica acta, 630(1), 82-90 (2008-12-11)
Broad-specific immunoassays are an emerging technology that allows simultaneous determination of a class of closely related small molecular weight analytes. The current approaches are mainly based on the competitive format and involve complicated or expensive processes hindering their wide application.
Danila Moscone et al.
Methods in molecular biology (Clifton, N.J.), 739, 217-235 (2011-05-14)
A novel method for aflatoxin B (AFB) determination is proposed. The AFB determination is based on acetylcholinesterase (AChE, from electric eel) inhibition, and the AChE residual activity is determined using the colorimetric method (Ellman's method). To select and optimize the
J C Netto-Ferreira et al.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 10(10), 1701-1708 (2011-08-13)
Aflatoxins are mycotoxins produced by fungi of the genus Aspergillus, which is widely spread in the tropics and subtropics. To date, aflatoxin phototoxicity has been recognized, but the mechanism responsible for this phototoxicity has not been fully characterized. In the
Sahib Alam et al.
Journal of food science, 75(2), M61-M64 (2010-05-25)
The efficacy of calcium propionate at 2 different doses (0.5% and 1%) against growth and aflatoxins production by Aspergillus flavus (A-2092) was investigated in vitro on Czapek yeast extract agar at different levels of water activity (a(w)) in the range
Marilena Muscarella et al.
Methods in molecular biology (Clifton, N.J.), 739, 203-210 (2011-05-14)
A high-performance liquid chromatographic method with on-line post-column photochemical derivatization and fluorimetric detection for the simultaneous separation and quantitative determination of aflatoxin (AF) B(1), B(2), G(1), and G(2) in foodstuffs and feed materials is reported.The chromatographic separation is accomplished by
Nicoleta Manuela Miliţă et al.
Bacteriologia, virusologia, parazitologia, epidemiologia (Bucharest, Romania : 1990), 55(1), 19-24 (2010-11-03)
Aflatoxins are secondary metabolites produced by a group of strains, mainly Aspergillus and Penicillium species. These mycotoxins are bifurano-coumarin derivatives group with four major products B1, B2, G1 and G2 according to blue or green fluorescence emitted in ultraviolet light
J Atehnkeng et al.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 25(10), 1264-1271 (2008-07-09)
Aflatoxin contamination resulting from maize infection by Aspergillus flavus is both an economic and a public health concern. Therefore, strategies for controlling aflatoxin contamination in maize are being investigated. The abilities of eleven naturally occurring atoxigenic isolates in Nigeria to
C Lorini et al.
Annali di igiene : medicina preventiva e di comunita, 20(6), 553-562 (2009-02-26)
Aim of this survey is to identify those filamentous fungi which parasite Boletus edulis and its group and check the potential presence of secondary metabolites, specifically aflatoxin B1, total aflatoxins and ochratoxin A, in order to assess the risk to
Lina Liu et al.
Phytochemical analysis : PCA, 23(5), 469-476 (2012-02-11)
We report a rapid and accurate method to determine the natural occurrence of aflatoxins (AFs) B1, B2, G1 and G2 in medicinal herbs and the analysis of 174 commercial samples by this method. The aim of the present work is
M I Almeida et al.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 29(4), 694-703 (2012-02-10)
A total of 230 samples of processed rice and its sub-products or derived products were analysed to establish the co-occurrence of several mycotoxins. Samples were analysed in the period 2007-2009 due to the outbreak of beriberi associated with the consumption
D Tang et al.
Biosensors & bioelectronics, 25(2), 514-518 (2009-08-25)
A novel membrane-based lateral-flow immunodipstick assay was developed for the fast screening of aflatoxin B(2) (AFB(2)) as a model compound in food samples. The detector reagent consisted of magnetic nanogold microspheres (MnGMs) with nano-Fe(2)O(3) particles as core and gold nanoparticles
J Bansal et al.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 28(6), 767-774 (2011-05-31)
Approximately 200 samples of rice (including white, brown, red, black, basmati and jasmine, as well as wild rice) from several different countries, including the United States, Canada, Pakistan, India and Thailand, were analysed for aflatoxins, ochratoxin A (OTA) and fumonisins
J Yang et al.
Poultry science, 91(11), 2792-2801 (2012-10-24)
The purpose of this study was to evaluate the effects of feeding corn naturally contaminated with aflatoxin B(1) (AFB(1)) and aflatoxin B(2) (AFB(2)) on serum biochemical parameters, hepatic antioxidant enzyme activities, and pathological lesions of broilers. In total, 1,200 Cobb
A A Kalinichenko et al.
Bioorganicheskaia khimiia, 36(1), 122-132 (2010-04-14)
A panel of ten monoclonal antibodies to aflatoxins B1, B2, and G2 was produced and comprehensively characterized. The affinity and cross reactivity of these antibodies were determined using the methods of direct, indirect, and competitive ELISA. The structures of monoclonal
Lei Bao et al.
Journal of AOAC International, 93(3), 936-942 (2010-07-16)
Edible oils are consumed directly, and used as ingredients in food, soaps, and skin products. However, oils such as olive oil, peanut oil, and sesame oil could be contaminated with aflatoxins, which are detrimental to human and animal health. A
Sergio José López Grío et al.
Journal of separation science, 33(4-5), 502-508 (2010-02-04)
A rapid and simple method was developed to determine aflatoxins B1, B2, G1, G2 and ochratoxin A in animal feed and pet foods by UHPLC-MS/MS. Because the complexity of the evaluated matrices, the proposed method is based on sonication extraction
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