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Gerold Schmitt-Ulms et al.
PloS one, 4(9), e7208-e7208 (2009-09-29)
In the more than twenty years since its discovery, both the phylogenetic origin and cellular function of the prion protein (PrP) have remained enigmatic. Insights into a possible function of PrP may be obtained through the characterization of its molecular
Helena Firczuk et al.
The FEBS journal, 287(5), 925-940 (2019-09-15)
Control of complex intracellular pathways such as protein synthesis is critical to organism survival, but is poorly understood. Translation of a reading frame in eukaryotic mRNA is preceded by a scanning process in which a subset of translation factors helps
Dominic Docter et al.
Nature protocols, 9(9), 2030-2044 (2014-08-01)
Nanoparticle applications in biotechnology and biomedicine are steadily increasing. In biological fluids, proteins bind to nanoparticles that form the protein corona, crucially affecting the nanoparticles' biological identity. As the corona affects in vitro and/or in vivo nanoparticle applications, we developed
Clifton K Fagerquist et al.
Rapid communications in mass spectrometry : RCM, 26(10), 1241-1248 (2012-04-14)
Matrix-assisted laser desorption/ionization (MALDI) time-of-flight-time-of-flight (TOF-TOF) post-source decay (PSD) tandem mass spectrometry (MS/MS) has seen increasing use for analysis of non-digested protein ions for top-down proteomic identification. However, there is no commonly accepted calibrant for this purpose beyond the use
Giuseppe Dionisio et al.
Molecules (Basel, Switzerland), 24(13) (2019-07-10)
Xenobiotic detoxification in plant as well as in animals has mostly been studied in relationship to the deactivation of the toxic residues of the compound that, surely for azoxystrobin, is represented by its β-methoxyacrylate portion. In maize roots treated for
Delphine Vincent et al.
International journal of molecular sciences, 23(2) (2022-01-22)
Bread wheat is the most widely cultivated crop worldwide, used in the production of food products and a feed source for animals. Selection tools that can be applied early in the breeding cycle are needed to accelerate genetic gain for
Ivo M B Francischetti et al.
Journal of proteome research, 9(8), 3820-3831 (2010-05-06)
The evolution of insects to a blood diet leads to the development of a saliva that antagonizes their hosts' hemostasis and inflammation. Hemostasis and inflammation are redundant processes, and thus a complex salivary potion composed of dozens or near 100
S B Bilezikian et al.
The Journal of clinical investigation, 56(2), 438-445 (1975-08-01)
Thrombin converts fibrinogen to fibrin monomer by cleaving fibrinopeptides A and B (FPA and FPB) from the amino terminal ends of the A (alpha) and B (beta) chains. A radioimmunoassay capable of measuring the A peptide in human blood as
Chiara Nitride et al.
Analytical and bioanalytical chemistry, 411(16), 3463-3475 (2019-05-30)
The unintentional presence of even trace amounts of certain foods constitutes a major hazard for those who suffer from food allergies. For many food industries, product and raw ingredient surveillance forms part of their risk assessment procedures. This may require
Timo Glatter et al.
Molecular systems biology, 5, 237-237 (2009-01-22)
Protein complexes represent major functional units for the execution of biological processes. Systematic affinity purification coupled with mass spectrometry (AP-MS) yielded a wealth of information on the compendium of protein complexes expressed in Saccharomyces cerevisiae. However, global AP-MS analysis of
Kristin E Burns et al.
The Journal of biological chemistry, 284(5), 3069-3075 (2008-11-26)
The striking identification of an apparent proteasome core in Mycobacteria and allied actinomycetes suggested that additional elements of this otherwise strictly eukaryotic system for regulated protein degradation might be conserved. The genes encoding this prokaryotic proteasome are clustered in an
Lukas N Mueller et al.
Proteomics, 7(19), 3470-3480 (2007-08-30)
Label-free quantification of high mass resolution LC-MS data has emerged as a promising technology for proteome analysis. Computational methods are required for the accurate extraction of peptide signals from LC-MS data and the tracking of these features across the measurements
Nicholas M Frame et al.
Journal of molecular biology, 432(7), 1978-1995 (2020-02-10)
Serum amyloid A (SAA) is a plasma protein that transports lipids during inflammation. To explore SAA solution conformations and lipid-binding mechanism, we used hydrogen-deuterium exchange mass spectrometry, lipoprotein reconstitution, amino acid sequence analysis, and molecular dynamics simulations. Solution conformations of
Meriem Ameziani et al.
The protein journal, 39(5), 574-590 (2020-09-23)
The current report focuses on purification, structural and functional characterization of Cerastategrin from Cerastes cerastes venom, a novel basic disintegrin (pI 8.36) with 128 amino acid residues and a molecular weight of 13 835.25 Da measured by MALDI-MSMS. The 3D structure
R M Senior et al.
The Journal of clinical investigation, 77(3), 1014-1019 (1986-03-01)
Fibrin formation and turnover are intimately associated with inflammation and wound healing. To explore whether fibrin(ogen)-derived peptides exert direct effects upon cells involved in inflammation and tissue repair we examined the capacity of human fibrinopeptide B (hFpB), a thrombin-derived proteolytic
Mitchell Acland et al.
Methods and protocols, 5(4) (2022-07-28)
The molecular analysis of small or rare patient tissue samples is challenging and often limited by available technologies and resources, such as reliable antibodies against a protein of interest. Although targeted approaches provide some insight, here, we describe the workflow
Ute Distler et al.
Nature protocols, 11(4), 795-812 (2016-03-25)
Unbiased data-independent acquisition (DIA) strategies have gained increased popularity in the field of quantitative proteomics. The integration of ion mobility separation (IMS) into DIA workflows provides an additional dimension of separation to liquid chromatography-mass spectrometry (LC-MS), and it increases the
D Andrew James et al.
Journal of pharmaceutical sciences, 107(12), 3022-3031 (2018-09-04)
HSV529 is a replication defective human herpes simplex virus (HSV)-2 viral vaccine candidate in clinical development. An engineered cell line is required to support production of HSV529 by transgenic expression of the HSV-1 transcription factors UL5 (HELI) and UL29 (DNBI).
Anthony W Purcell et al.
Nature protocols, 14(6), 1687-1707 (2019-05-17)
Peptide antigens bound to molecules encoded by the major histocompatibility complex (MHC) and presented on the cell surface form the targets of T lymphocytes. This critical arm of the adaptive immune system facilitates the eradication of pathogen-infected and cancerous cells
Johnson Agniswamy et al.
Acta crystallographica. Section D, Biological crystallography, 64(Pt 4), 354-367 (2008-04-09)
Heavy-atom derivatization is routinely used in protein structure determination and is thus of critical importance in structural biology. In order to replace the current trial-and-error heavy-atom derivative screening with a knowledge-based rational derivative-selection method, the reactivity of more than 40
Joseph A Newman et al.
Life science alliance, 4(1) (2020-11-18)
Loss of WRN, a DNA repair helicase, was identified as a strong vulnerability of microsatellite instable (MSI) cancers, making WRN a promising drug target. We show that ATP binding and hydrolysis are required for genome integrity and viability of MSI
Ann M Cathcart et al.
Nature chemical biology, 16(11), 1218-1226 (2020-08-19)
The ubiquitin-proteasome system (UPS) is a highly regulated protein disposal process critical to cell survival. Inhibiting the pathway induces proteotoxic stress and can be an effective cancer treatment. The therapeutic window observed upon proteasomal blockade has motivated multiple UPS-targeting strategies
Andrew T Crombie et al.
Nature, 510(7503), 148-151 (2014-04-30)
The climate-active gas methane is generated both by biological processes and by thermogenic decomposition of fossil organic material, which forms methane and short-chain alkanes, principally ethane, propane and butane. In addition to natural sources, environments are exposed to anthropogenic inputs
Chao Wang et al.
The Journal of biological chemistry, 285(35), 26788-26797 (2010-06-03)
Protein disulfide isomerase (PDI), which consists of multiple domains arranged as abb'xa'c, is a key enzyme responsible for oxidative folding in the endoplasmic reticulum. In this work we focus on the conformational plasticity of this enzyme. Proteolysis of native human
Lawrence M Schopfer et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 878(17-18), 1297-1311 (2009-09-19)
We have identified organophosphorus agent (OP)-tyrosine adducts on 12 different proteins labeled with six different OP. Labeling was achieved by treating pure proteins with up to 40-fold molar excess of OP at pH 8-8.6. OP-treated proteins were digested with trypsin
Kélen Fabíola Arroteia et al.
PloS one, 9(8), e103566-e103566 (2014-08-02)
The epididymis has an important role in the maturation of sperm for fertilization, but little is known about the epididymal molecules involved in sperm modifications during this process. We have previously described the expression pattern for an antigen in epididymal
Dominic Narang et al.
Journal of the American Society for Mass Spectrometry, 32(7), 1593-1600 (2021-04-02)
The novel severe respiratory syndrome-like coronavirus (SARS-CoV-2) causes COVID-19 in humans and is responsible for one of the most destructive pandemics of the last century. At the root of SARS-CoV infection is the interaction between the viral spike protein and
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