Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can
Reliable histology staining requires consistent quality pure water. See examples of GMS and H&E stains performed with various water contaminants and comparing tap vs Type 2 water.