Histology

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facet applications:Histology

facet content type:Technical Article

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Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Centrifugation Separations

CsCl gradient centrifugation separates RNA from DNA; differential and density gradient centrifugation techniques explained.

Tissue Markers for Cancer Diagnostics

Immunohistochemistry (IHC) represents an indispensable tool in cancer diagnostics.

Staining for Fungi

Fungal stains remain an important tool in the histology laboratory's diagnostic arsenal for identifying infectious microorganisms.

Water for Histology Staining

Reliable histology staining requires consistent quality pure water. See examples of GMS and H&E stains performed with various water contaminants and comparing tap vs Type 2 water.

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

离心分离

差速离心法和密度梯度离心法是两种离心式颗粒分离技术。CsCl梯度离心法（氯化铯离心法）用来制备从DNA中密度梯度离心分离RNA的溶液。