CN
Imaging analysis and live cell imaging
应用筛选条件
facet applications:Imaging analysis and live cell imaging
facet content type:Protocol
显示 1-8 共 8 条结果
方案指导：使用PKH亲脂性膜染料标记外泌体
PKH染料已成功用于体外和体内跟踪实验中标记外泌体和细胞外囊泡。下述外泌体标记方案详细介绍了在微囊泡追踪实验中使用PKH染料实现可靠的外泌体荧光标记的步骤。
细胞免疫荧光标记
细胞染色可分为四个步骤：细胞制备、固定、抗体孵育和评估。
Microscopic Examination of Samples
This protocol covers 3 modes for the microscopic examination of cell samples.
Cell Capture Imaging Reagent
Immunocytochemistry (ICC) Cell Capture Imaging Reagent and protocol for eliminating wash steps, and is highly effective in applications involving rare cells, low cell count samples, and limited access samples.
Washing Microparticles
Microparticles protocol for washing particles may be done via centrifugation. This procedure must be performed carefully.
Protocol Guide: Exosome Labeling Using PKH Lipophilic Membrane Dyes
PKH dyes have been successfully used to label exosomes and extracellular vesicles for in vitro and in vivo tracking experiments. The exosome labeling protocol below details steps for reliable fluorescent labeling of exosomes using PKH dyes for microvesicle tracking experiments.
Toluidine Blue Staining Protocol for Millicell® Culture Inserts
Toluidine blue stains the nuclear material in tissues with a high DNA and RNA content and is used for selectively staining acidic tissue components such as carboxylates, sulfates, and phosphate radicals.
Immunofluorescence Labeling of Cells
Cell staining can be divided into four steps: cell preparation, fixation, application of antibody, and evaluation.