Nucleic acid labeling and detection

应用筛选条件

facet applications:Nucleic acid labeling and detection

facet content type:Protocol

显示 1-10 #N/A 19 条结果

Gelatin Blocking Buffer for Direct Detection Southern or Western Blotting

In order to specifically detect an antigen or target molecule immobilized on a solid support, unoccupied binding sites on the support must be blocked against binding by probe and detection molecules.

DIG Easy Hyb™ Granules Protocol & Troubleshooting

DIG Northern Starter Kit Protocol & Troubleshooting

DNA probes, like PCR or random-primed DNA probes, are effective for northern blots, particularly for detecting abundant messages.

DIG RNA Labeling Kit (SP6/T7) Protocol & Troubleshooting

Determine the labeling efficiency in terms of μg (expected yield of a standard labeling reaction is 20 μg of DIG labeled RNA per μg linearized template DNA after the DIG RNA labeling reaction).

DIG RNA Labeling Mix Protocol Troubleshooting

Determine the labeling efficiency in terms of μg (expected yield of a standard labeling reaction is 20 μg of DIG labeled RNA per μg linearized template DNA after the DIG RNA labeling reaction).

NBT/BCIP Ready-to-Use Tablets Protocol Troubleshooting

NBT/BCIP Stock Solution Protocol & Troubleshooting

NBT Protocol

NBT Protocol & Troubleshooting

There are several counterstains possible in combination with BM Purple (or NBT/BCIP in general), including FastGreen FCF and Nuclear Fast Red.

Oligonucleotide Melting Temperature

Learn about methods for calculating oligonucleotide melting temperature (Tm).

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