Robust Taq DNA polymerase reagents and PCR kits for efficient extraction, purification, and amplification of routine and challenging DNA template sequences.
Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.
Perform reverse transcription (RT) using a reverse transcriptase enzyme and dNTPs. Use total RNA or a gene-specific approach so that only the RNA of interest is converted to cDNA.