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Protein concentration and buffer exchange
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facet applications:Protein concentration and buffer exchange
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Rapid Antibody Concentration with Amicon® Ultra Centrifugal Filters
Purification protocols for immunoglobulins generally include affinity binding to Protein A or G chromatography media, washing unbound proteins, and eluting with a low pH buffer.
Rapid, Ultrafiltration-based Antibody Purification
Ultrafiltration-based MAb purification offers faster, higher recovery compared to hybridoma screening.
Sample Preparation for Size Exclusion Chromatography
Ensure chromatographic sample clarity to avoid column clogging, reduce washing needs, and prolong column life for purification.
A Simple Strategy for Protein Enrichment Using Ultrafiltration
The human plasma proteome is a vast resource for biomarker discovery and investigation.
Amicon® Stirred Cells
User guide for Amicon® Stirred cells including features, operation, specifications, and troubleshooting.
Buffer Reference Center
These tables will help you navigate the preparation of many common buffer solutions by pH and pKa. Choose a buffer with an effective pH range for your specific assay.
Conditioning Purified Membrane Proteins
This page shows how to condition membrane proteins for further analysis with products from Cytiva.
Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
Desalting Samples with Mini Dialysis Kit
Mini Dialysis Kit from Cytiva minimizes sample handling and loss, reducing streaking on 2-D gels from contaminants.
Detergent Removal with Amicon® Pro Centrifugal Filters
Detergents are frequently used to solubilize proteins and nucleic acids during purification, but the presence of detergents may interfere with downstream analyses.
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