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qPCR
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facet applications:qPCR
facet content type:Protocol
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通用SYBR Green qPCR实验方案
我们的SYBR Green qPCR实验方案是一种旨在准确定量基因表达和RT-PCR反应的的方法
选择qPCR参照基因的实验方案
基因表达数据的分析需要稳定的参考或上样控制。所述参考通常是一个或多个参照基因。
引物浓度优化实验方案
引物浓度优化实验方案是一种建立反应阵列的方法。该阵列用来对照伴侣引物的不同浓度来测试每种引物的一系列浓度。
使用温度梯度的引物优化试验方案
用于测定优化的梯度PCR通过在一系列退火温度下测试含有固定引物浓度的相同反应物来确定引物的最佳退火温度（Ta）。
NHS-酯修饰与氨基标记的寡核苷酸缀合的实验方案
NHS-酯修饰与氨基标记的寡核苷酸缀合的实验方案
qPCR效率测定实验方案
QPCR 条件的优化对于制定稳健的检测方案非常重要。两种主要优化方法是优化引物浓度和/或退火温度。
FFPE 组织
存档的福尔马林固定和石蜡包埋 (FFPE) 组织样本是分析基因表达和研究多种疾病的宝贵资源。
使用SYBR Green I染料检测的qPCR基因表达/拷贝数分析实验方案
使用 SYBR Green I 染料检测一个或多个稳定内参基因的靶量是qPCR的常见应用。下面是一个可以适应特定实验需要的标准实验方案。
KiCqStart™通用SYBR® Green qPCR实验方案
使用SYBR Green试剂的定量PCR实验方案。该方法支持绝大多数qPCR仪器。
ABI3900 Synthesizer Columns with CUTAG CPG Frits
Next generation CPG Frit support for reliable, cost-effective oligo synthesis from Proligo®, for ABI 3900 and MerMade synthesizers
Rapid SYBR® Green qPCR on the Illumina Eco™ Real-Time PCR System
Fast real-time PCR is a demanding application that requires consistent and reproducible results from the difficult amplicons and low-volume reactions
High-throughput Real-Time PCR: SYBR® Green
High-throughput qPCR using SYBR® Green is a demanding application that requires consistent and reproducible results from low-volume, high-speed assays.
MystiCq® MicroRNA® Quantitation System
Method for purification, reverse transcription and quantitative PCR for MicroRNAs using Mysticq reagents
RT-qPCR Basic Troubleshooting
Learn the basics of real-time PCR and qPCR with tips, tricks and general troubleshooting.
Protocol for Conjugating NHS-Ester Modifications to Amino-Labeled Oligonucleotides
Protocol for conjugating NHS-ester modifications to an oligonucleotide with an amino label.
qPCR Reference Gene Selection Protocol
Analysis of gene expression data requires a stable reference or loading control. This reference is usually one or more reference genes.
Reverse Transcription Protocol (One-step Probe Detection)
Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
KiCqStart™ Universal SYBR® Green qPCR Protocol
Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.
KAPA SYBR® FAST One-Step qRT-PCR Kits FAQs
Frequently asked questions (FAQs) for KAPA SYBR® FAST One-Step qRT-PCR Kits.
Multiplex qPCR Protocol
Standard qPCR protocol with up to four detection probes at specified concentrations, simplifying reaction setup with LuminoCt ReadyMix.
qPCR Gene Expression Protocol Using SYBR Green
SYBR Green I dye in qPCR measures target quantity, adaptable to specific needs with a standard protocol.
qPCR with Single Detection Probe
qPCR protocol template with specific detection probes aids in single-target detection using LuminoCt® ReadyMix™.
SPUD Assay for Detection of Assay Inhibitors Protocol
SPUD assay identifies inhibitors in RNA or DNA samples, useful for analyzing numerous or low-copy targets.
Primer Optimization Using Temperature Gradient
Gradient PCR optimizes assay conditions by testing fixed primer concentrations across various annealing temperatures.
Multiplex Real-Time PCR
Multiplex qPCR employing probe-based chemistries is a demanding application that often requires extensive optimization and validation.
SYBR® Green JumpStart™ Taq ReadyMix™
Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.
RNA Immunoprecipitation qPCR (RIP- qPCR) Protocol
RNA immunoprecipitation (RIP) can identify specific RNA molecules of many types associated with specific nuclear or cytoplasmic binding proteins.
qPCR Efficiency Determination Protocol
Optimization of qPCR conditions is important for the development of a robust assay. The two main approaches are optimization of primer concentration and/or annealing temperatures.
Universal SYBR Green qPCR Protocol
Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions
KiCqStart™ Probe qPCR ReadyMix™
KiCqStart™ Probe qPCR ReadyMix™ is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments.
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