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Western blotting
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facet applications:Western blotting
facet content type:Protocol
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使用BCIP / NBT底物进行免疫检测
5-溴-4-氯-3-吲哚基磷酸盐（BCIP）/ 硝基蓝四唑（NBT）是一种理想的不溶性底物，可与碱性磷酸酶一起使用。该系统产生蓝紫色产物。可以目视观察到强烈的颜色，非常稳定，并且在暴露于光时不褪色。
用于直接检测型Southern或Western Blotting的明胶封闭缓冲液
为了特异性地检测固定在固体支持物上的抗原或靶分子，必须将支持物上未被占据的结合位点进行封闭以防止探针和检测分子结合。
免疫印迹膜剥离和再生检测
本页内容介绍和讨论了三种免疫印迹膜剥离和重新检测的方案。
WB检测样本前处理：细胞裂解和蛋白提取
Western blotting前用于培养组织和细胞的细胞裂解和有效蛋白提取的样品制备实验方案。
Western Blotting实验方法 - 免疫印迹或Western Blot实验方法
带有用于不同印迹过程工作流程步骤的Western blot实验方法，描述了蛋白从SDS聚丙烯酰胺凝胶(SDS-PAGE)到硝酸纤维素膜(NC膜)的电泳转移。也称为免疫印迹实验方法。
Prestige Antigens™—封闭实验方案
推荐按照标准的Prestige Antigen封闭实验方案使用Prestige Antigens™以及相应的Prestige Polyclonals或Monoclonals
PAGE简介 — 基于分子大小的蛋白质分离
PAGE简介了解基于分子大小利用聚丙烯酰胺凝胶分离蛋白质的SDS-PAGE背景和试验方案。
Chemiluminescence Detection with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select
This page describes chemiluminescence detection of GST-tagged proteins with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select from Cytiva.
30-minute Immunodetection Protocol Using the SNAP i.d.® 2.0 System
The SNAP i.d. 2.0 Protein Detection System is the second generation of the SNAP i.d. method for detecting immunoreactive proteins on Western blots.
Stripping and Reprobing Western Blotting Membranes
This page shows and discusses three protocols for stripping and reprobing a western blot membrane.
Colorimetric Detection of Western blot using 4-Chloro-1-Naphthol Solution Protocol
This solution is used as a substrate for detecting horseradish-peroxidase conjugates in Western blotting.
Introduction to Western Blot Detection of Proteins
Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.
Introduction to SDS-PAGE - Separation of Proteins Based on Size
Introduction to PAGE. Learn about SDS-PAGE background and protocol for the separation of proteins based on size in a poly-acrylamide gel.
Prestige Antigens™—Blocking Protocol
Prestige Antigens™ and the corresponding Prestige Polyclonals or Monoclonals are recommended to use according to the standard Prestige Antigen-blocking protocol
Casein Blocking Buffer Protocol for Southern or Western Blotting
Protocol for using Casein Blocking Buffer in Southern and Western blotting applications, including colorimetric and chemiluminescent substrate detection options. Troubleshooting for optimizing signal and reducing background is also included.
Detection of GST-tagged Proteins with Western Blot
This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using Cytiva products.
Immunodetection Using BCIP/NBT Substrate
5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) is an ideal insoluble substrate for use with alkaline phosphatase. This system produces a blue-purple product. The intense color can be observed visually, is very stable, and will not fade upon exposure to light.
Gelatin Blocking Buffer for Direct Detection Southern or Western Blotting
In order to specifically detect an antigen or target molecule immobilized on a solid support, unoccupied binding sites on the support must be blocked against binding by probe and detection molecules.
Cell Lysis and Protein Extraction for Western Blotting
Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.
Western Blotting Protocol - Immunoblotting or Western Blot Protocol
Western blot protocol with workflow steps for different blot procedures, describing the electrophoretic transfer of proteins from SDS polyacrylamide gels to sheets of nitrocellulose. Also known as Immunoblotting protcol.
Western Blotting Sample Preparation
Rules and good practice in sample preparation for Western blot sample preparation from cell culture and tissue samples.