Prepare Filter for Rinse
Solvent
Unscrew the two sections of
the 25 mm filter holder, and
use forceps to center a
25 mm white filter disc on the
perforated plastic support in
the lower section of the
to easily remove it using your forceps.
Utilizing sterile forceps, peel back the film and separate the blue paper
from the membrane. Remove the membrane with the forceps.
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Utilizing sterile
forceps, peel
back the film and
separate the blue
paper from the
membrane. Remove
the membrane
with the forceps.
Place the membrane
and blue paper
on
Required
● Filter appropriate for your application; see the product catalogue for options
● Filter forceps (cat. no. XX6200006P)
● For filter holders with a silicone stopper, a vacuum filtering flask or
with gentle
agitation. Use 1 ml/cm
2
of membrane.
C. Washing the Membrane
1. Using forceps, transfer the membrane to a clean
container containing 1–1.5 ml Wash Buffer 1/cm
2
of membrane
temperature with gentle
agitation. Use 1 ml/cm2 of membrane.
C. Washing the Membrane
1. Using forceps, transfer the membrane to a clean
container containing Wash Buffer 1. Use
1-1.5 ml/cm2 of membrane
or magnesium to each plate.
3. Using sterile, curved forceps, center the cylinder over a colony of cells. Using the flat side of the
forceps, press gently but firmly on the cylinder to create a seal
base. Use sterile
forceps to remove the filter. Transfer filter
to appropriate media or examine filtrate as
required by your application.
Unscrew the funnel from the base. Use forceps
to remove the
bottle, or pour used solution back into
the substrate bottle. Use clean gloves and forceps; do
not use rusted forceps. Analytes can be applied to
membranes as a dot blot or by gel transfer. Blotting
exposure cassette, Product No.
E 9260, E 9385, E 9510, E 9635
• Plastic development folders
• Forceps
Precautions and Disclaimer
The CDP-Star Universal Kit is for laboratory use only.
Not for drug
top center of the disc. With membrane forceps,
rub from the edge of the disc inward to lift the edge of the disc.
2. Pull the disc off the sheet with the forceps.
3. Center the disc on the top
to adjust to room temperature in a tissue culture
hood.
2. Sterilize forceps with 70% ethanol and handle inserts with forceps.
3. Add 300 µl of warm serum free media to the interior of the inserts
90 mm filter appropriate for your application; see the product catalogue for options
● Filter forceps (cat. no. XX6200006P)
● Vacuum filtering flask with ground glass attachment. Refer to Ordering
or multiwell
plate for extractions (200 µL minimal well volume)
• Small dissecting scissors
• Forceps (small to medium in size)
• Buccal swab - Sterile foam tipped applicator,
Catalog Number A9601
barrel. Use smooth-tip forceps
to remove the top cap O-ring and filter sealing O-ring. To remove the filter
sealing O-ring, gently pry it out of the base by inserting the forceps into the
small rounded
contents equally onto the absorbent pad.
3. Set up the membrane filtration apparatus. Use sterile forceps to put the membrane filter in the assembly.
The grid side is up.
4. Invert the sample / diluted
contents equally onto the absorbent pad.
3. Set up the membrane filtration apparatus. Use sterile forceps to put the membrane filter in the assembly.
The grid side is up.
4. Invert the sample / diluted
contents equally onto the absorbent pad.
3. Set up the membrane filtration apparatus. Use sterile forceps to put the membrane filter in the assembly. The
grid side is up.
4. Invert the sample / diluted
contents equally onto the absorbent pad.
3. Set up the membrane filtration apparatus. Use sterile forceps to put the membrane filter in the assembly.
The grid side is up.
4. Invert the sample / diluted
plates to room temperature (25°C) prior to initiating assay.
2. Sterilize forceps with 70% ethanol and handle inserts with forceps.
3. Add 300 μL of prewarmed serum free media to the interior of the inserts
.5 or 2 mL) or multi-well plate for extractions
• Small dissecting scissors or scalpel
• Forceps (small to medium in size)
• Buccal swab (Sterile foam tipped applicator, Cat. No. WHAWB100032
.5 or 2 mL) or multi-well plate for extractions
• Small dissecting scissors or scalpel
• Forceps (small to medium in size)
• Buccal swab (Sterile foam tipped applicator, Cat. No. WHAWB100032