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Rahul Kashyap et al.
Bioprocess and biosystems engineering, 38(12), 2417-2426 (2015-10-07)
Experimental investigations were made to synthesize fructo-oligosaccharides (FOS) from sucrose using fructosyltransferase. The influence of various parameters such as temperature (45-55 °C), pH (4-5), initial sucrose concentration (ISC: 300-500 g/L) and enzyme concentration (4-32 U/mL) were varied. A maximum FOS
Fang Xie et al.
International journal of biological macromolecules, 126, 525-530 (2018-12-28)
Starch-containing food normally has complex components and structure, which control its digestion kinetic properties and nutritional value. However, interactions among multiple food components and their effects on starch digestion kinetics are rarely reported. Here, we evaluated the influence of tea
Rocco Moretti et al.
Analytical biochemistry, 377(2), 251-258 (2008-04-05)
A systematic comparison of six sugar indicators for their sensitivity, specificity, cross-reactivity, and suitability in the context of crude lysates revealed para-hydroxybenzoic acid hydrazide (pHBH) to be best suited for application in a plate-based phosphatase-assisted universal sugar-1-phosphate nucleotidyltransferase assay. The
Extrusion induced low-order starch matrices: Enzymic hydrolysis and structure
Zhang B, et al.
Carbohydrate Polymers, 134(10), 485-496 (2015)
Measurement of total fructan in foods by enzymatic/spectrophotometric method: collaborative study
McCleary BV, et al.
Journal of AOAC (Association of Official Analytical Chemists) International, 83(2), 356-364 (2000)
Li Ding et al.
Food & function, 10(9), 5312-5322 (2019-07-30)
Modulation of the starch digestion rate and extent is a major target for increasing nutritional value of potato-based food. In order to understand the effect of controlled gelatinization on the structure and in vitro digestion properties of whole potato food
Electrophoretic deposition to promote layer-by-layer assembly for in situ gene delivery application.
Wei-Wen Hu et al.
Colloids and surfaces. B, Biointerfaces, 133, 171-178 (2015-06-24)
In this study, layer-by-layer (LbL) assembly of polyelectrolyte was facilitated using electric field assistance (EFA). To elucidate the EFA effects on polyelectrolyte multilayers (PEMs), the electric fields were solely administrated to either chitosan or DNA adsorption. Both DNA and chitosan
Starch digestion in intact pulse cells depends on the processing induced permeability of cell walls.
Ping Li et al.
Carbohydrate polymers, 225, 115204-115204 (2019-09-16)
Enzyme digestion of starch granules encapsulated within cell walls in pulse depends on both the intactness of cellular structure as well as the retention of the ordered structure of starch during processing. However, the role of cell wall permeability in
Yong-Chun Liu et al.
Journal of biochemistry, 147(3), 381-391 (2009-11-17)
The Er(III) complexes are prepared from Er(NO(3))(3).6H(2)O and Schiff-base ligands derived from 8-hydroxyquinoline-2-carboxaldehyde with four aroylhydrazines, including benzoylhydrazine, 2-hydroxybenzoylhydrazine, 4-hydroxybenzoylhydrazine and isonicotinylhydrazine, respectively. X-ray crystal and other structural analyses indicate that Er(III) and every ligand can form a binuclear Er(III)
Trine Holst Sørensen et al.
The Journal of biological chemistry, 290(36), 22193-22202 (2015-07-18)
We measured hydrolytic rates of four purified cellulases in small increments of temperature (10-50 °C) and substrate loads (0-100 g/liter) and analyzed the data by a steady state kinetic model that accounts for the processive mechanism. We used wild type
Fang Xie et al.
International journal of biological macromolecules, 126, 525-530 (2018-12-28)
Starch-containing food normally has complex components and structure, which control its digestion kinetic properties and nutritional value. However, interactions among multiple food components and their effects on starch digestion kinetics are rarely reported. Here, we evaluated the influence of tea
M Lever et al.
Analytical biochemistry, 139(1), 205-211 (1984-05-15)
4- Hydroxybenzoylhydrazine ( PAHBAH ) reacts with glucose in hot aqueous solution when alkali exceeds aroylhydrazine concentration. The related 2- furoylhydrazine ( FAH ) reacts at lower alkali concentrations, making this an attractive alternative carbohydrate reagent since it is (unlike
Laura Roman et al.
Carbohydrate polymers, 215, 198-206 (2019-04-15)
This work provides information on the feasibility of molecular shear scissions that promote interactions resulting in slowly digestible foods. Extrusion using nine different Specific Mechanical Energy (SME) was performed to obtain amylose and amylopectin with different hydrodynamic radius (Rh) and
Hanbin Xu et al.
International journal of biological macromolecules, 145, 527-534 (2019-12-25)
The interaction of polyphenolic catechins from Camellia sinensis tea with α-amylase, and the effects of the interactions on the kinetics of starch amylolysis, were investigated. Binding studies using surface plasmon resonance (SPR) and enzyme kinetic analysis indicated that the in
Adil Muhammad Farooq et al.
International journal of biological macromolecules, 118(Pt A), 160-167 (2018-06-18)
The aim of this study was to identify the contributions made by size fractions of four milled rice (i.e., waxy, white, black and brown rice) to structural and in vitro starch digestion properties after cooking. Rice grains were hammer-milled in
Barry V McCleary et al.
Journal of AOAC International, 102(3), 883-892 (2019-01-17)
Traditional enzyme-based methods for measurement of fructan were designed to measure just inulin and branched-type (agave) fructans. The enzymes employed, namely exo-inulinase and endo-inulinase, give incompletely hydrolysis of levan. Levan hydrolysis requires a third enzyme, endo-levanase. This paper describes a
Efficacy of reducing sugar and phenol-sulfuric acid assays for analysis of soluble carbohydrates in feedstuffs
Hall, Mary Beth
Anim. Feed Sci. Technol., 185(1-2), 94-100 (2013)
A Jerlich et al.
Biochimica et biophysica acta, 1481(1), 109-118 (2000-09-27)
In this study, the production of the highly toxic oxidant hypochlorous acid (HOCl) by the phagocytic enzyme myeloperoxidase (MPO) was quantitated and the concomitant alterations of low density lipoprotein (LDL) were analyzed in view of the potential role of LDL
Phedias Diamandis et al.
Nature chemical biology, 3(5), 268-273 (2007-04-10)
The identification of self-renewing and multipotent neural stem cells (NSCs) in the mammalian brain holds promise for the treatment of neurological diseases and has yielded new insight into brain cancer. However, the complete repertoire of signaling pathways that governs the
M Lever et al.
Analytical biochemistry, 144(1), 6-14 (1985-01-01)
Hydroxymethylfurfural and furfural can be estimated automatically at 60 samples/h in a continuous-flow system. The hydrazone of the furfural with 5-hydroxybenzoylhydrazine is formed in aqueous solution and dialyzed into methanol or other lower alcohols on the recipient side of the
Glucoamylase (exo-1, 4-alpha-d-glucan glucanohydrolase, EC 3.2. 1.3) is the major starch-degrading enzyme secreted by the phytopathogenic fungus Colletotrichum gloeosporioides
Krause DR, et al
Microbiology, 137(10), 2463-2468 (1991)
Ping Li et al.
Food chemistry, 315, 126268-126268 (2020-02-06)
Starch digestion in pulse cellular matrices is primarily determined by the hindrance of cell walls limiting enzyme diffusion as well as the retention of starch granular structure. However, the effect of hydrothermal treatment on structure and digestion properties of entrapped
Kang Xu et al.
Food chemistry, 317, 126387-126387 (2020-02-23)
The outstanding amount of phenolics and pectins of okra seeds and seedless pods, respectively, is well-known. However, their impact on bread nutritional quality, and particularly on slowing down α-amylase activity during crumb digestion, has never been studied. In this work
Dongling Qiao et al.
Carbohydrate polymers, 144, 271-281 (2016-04-17)
Combined analytical techniques were used to explore the effects of alkali treatment on the multi-scale structure and digestion behavior of starches with different amylose/amylopectin ratios. Alkali treatment disrupted the amorphous matrix, and partial lamellae and crystallites, which weakened starch molecular
Deepa C Khandekar et al.
Bioprocess and biosystems engineering, 37(12), 2529-2537 (2014-06-19)
The study reports the synthesis of fructo-oligosaccharide (FOS) from sucrose using invertase derived from Saccharomyces cerevisiae. The reaction was conducted in a batch mode under free enzyme condition. Fructo-oligosaccharide formation was detected at a high sucrose concentration of over 200 g/L.
Usman Ghani et al.
Bioorganic & medicinal chemistry, 18(11), 4042-4048 (2010-05-11)
A series of 1,3,4-thiadiazole-2(3H)-thiones, 1,3,4-oxadiazole-2(3H)-thiones, 4-amino-1,2,4-triazole-5(4H)-thiones, and substituted hydrazides were tailored and synthesized as new potent inhibitors of tyrosinase. The rationale for inhibitor design was based on the active site structural evidence from the crystal structures of bacterial tyrosinase and
Stefan Becker et al.
Bio-protocol, 8(8), e2666-e2666 (2018-04-20)
The marine beta-glucan laminarin is an abundant storage polysaccharide in microalgae. High production rates and rapid digestion by heterotrophic bacteria turn laminarin into an ideal carbon and energy source, and it is therefore a key player in the marine carbon
Laura Roman et al.
Carbohydrate polymers, 246, 116633-116633 (2020-08-05)
A structure-digestion model is proposed to explain the formation of α-amylase-slowly digestible structures during amylopectin retrogradation. Maize and potato (normal and waxy) and banana starch (normal and purified amylopectin through alcohol precipitation), were analyzed for amylose ratio and size (HPSEC)
Andrea Mellitzer et al.
Biotechnology journal, 7(1), 155-162 (2011-05-04)
The exploitation of renewable resources for the production of biofuels relies on efficient processes for the enzymatic hydrolysis of lignocellulosic materials. The development of enzymes and strains for these processes requires reliable and fast activity-based screening assays. Additionally, these assays
Martin J Baumann et al.
Analytical biochemistry, 410(1), 19-26 (2010-11-16)
In lignocellulosic raw materials for biomass conversion, hemicelluloses constitute a substantial fraction, with xylan being the primary part. Although many pretreatments reduce the amount or change the distribution of xylan, it is important to degrade residual xylan so as to
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