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Bo Zhu et al.
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 156(2), 95-103 (2012-05-15)
Ferritin, an iron storage protein, plays a key role in iron metabolism in vivo. Here, we have cloned an inducible ferritin cDNA with 519 bp within the open reading frame fragment from the hepatopancreas of Aplysia juliana (AJ). The subunit
Thomas K Karikari et al.
ACS chemical neuroscience, 11(19), 3117-3129 (2020-08-25)
Tau neurofibrillary tangles are key pathological features of Alzheimer's disease and other tauopathies. Recombinant protein technology is vital for studying the structure and function of tau in physiology and aggregation in pathophysiology. However, open-source and well-characterized plasmids for efficiently expressing
Kah Yan How et al.
PeerJ, 3, e1117-e1117 (2015-08-21)
Quorum sensing is a mechanism for regulating proteobacterial gene expression in response to changes in cell population. In proteobacteria, N-acyl homoserine lactone (AHL) appears to be the most widely used signalling molecules in mediating, among others, the production of extracellular
Vendula Studentova et al.
Folia microbiologica, 60(2), 119-129 (2014-09-30)
Carbapenemase-mediated resistance to carbapenems in Enterobacteriaceae has become the main challenge in the treatment and prevention of infections recently. The partially unnoticed spread of OXA-48-type carbapenemase producers is usually assigned to low minimum inhibitory concentrations (MICs) of carbapenems that OXA-48-producing
David R Jones et al.
Nature protocols, 8(6), 1058-1072 (2013-05-11)
Phosphoinositides represent a minor fraction of the total glycerolipids in cells. Despite the fact that phosphoinositides are present in small quantities, they have crucial roles during cell signaling and in regulating numerous intracellular processes. Measuring changes in the levels of
Liang Kong et al.
Molecular cell, 81(22), 4591-4604 (2021-10-01)
Protein ADP-ribosylation is a reversible post-translational modification that transfers ADP-ribose from NAD+ onto acceptor proteins. Poly(ADP-ribosyl)ation (PARylation), catalyzed by poly(ADP-ribose) polymerases (PARPs) and poly(ADP-ribose) glycohydrolases (PARGs), which remove the modification, regulates diverse cellular processes. However, the chemistry and physiological functions
Lei Tang et al.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology, 28(10), 1094-1097 (2012-10-11)
To construct an expression plasmid for NY-ESO-1 gene and identify the expression of recombinant protein NY-ESO-1/GST in E.coli. NY-ESO-1 segment was amplified from the testis cDNA library by RT-PCR and cloned into the prokaryotic expression vector pGEX4T-1 downstream tagged by
Claudia Rehwald et al.
British journal of cancer, 122(3), 421-433 (2019-11-28)
We aimed at clarifying the role of lipocalin-2 (LCN-2) in clear-cell renal cell carcinoma (ccRCC). Since LCN-2 was recently identified as a novel iron transporter, we explored its iron load as a decisive factor in conferring its biological function. LCN-2
Cristina Basilico et al.
The Journal of clinical investigation, 124(7), 3172-3186 (2014-05-29)
Activation of MET by HGF plays a key role in tumor progression. Using a recently developed llama platform that generates human-like immunoglobulins, we selected 68 different antibodies that compete with HGF for binding to MET. HGF-competing antibodies recognized 4 distinct
Miyuki Tsukuda et al.
Journal of bioscience and bioengineering, 120(5), 591-595 (2015-04-29)
Counterselection is a genetic engineering technique to eliminate specific genetic fragments containing selectable marker genes. Although the technique is widely used in bacterial genome engineering and plasmid curing experiments, the repertoire of the markers usable in Escherichia coli is limited.
Namil Lee et al.
iScience, 24(12), 103410-103410 (2021-12-09)
Streptomyces species have attracted considerable interest as a reservoir of medically important secondary metabolites, which are even diverse and different between strains. Here, we reassess ten Streptomyces venezuelae strains by presenting the highly resolved classification, using 16S rRNA sequencing, MALDI-TOF
Anita C Parker et al.
Plasmid, 68(2), 86-92 (2012-04-11)
The genus Bacteroides are gram-negative, obligate anaerobes indigenous to the gastrointestinal tract of humans and animals. The Bacteroides and other members of the Bacteroidetes phylum have diverged from the Proteobacteria. These organisms evolved a unique promoter structure for the initiation
Daesik Kim et al.
Nature protocols, 16(2), 1170-1192 (2021-01-20)
Digested genome sequencing (Digenome-seq) is a highly sensitive, easy-to-carry-out, cell-free method for experimentally identifying genome-wide off-target sites of programmable nucleases and deaminases (also known as base editors). Genomic DNA is digested in vitro using clustered regularly interspaced short palindromic repeats
Farzaneh Zahabiun et al.
The American journal of tropical medicine and hygiene, 93(2), 319-325 (2015-06-03)
Toxocariasis is a cosmopolitan zoonotic disease caused by the infective larvae of Toxocara canis and T. cati. Diagnosis in humans is usually based on clinical symptoms and serology. Immunoglobulin G (IgG)-enzyme-linked immunosorbent assay kits using T. canis excretory-secretory (TES) larval
Shuxia Fan et al.
Bio-protocol, 8(18), e3018-e3018 (2018-09-20)
Blood platelets are critical for hemostasis and thrombosis, but also play diverse roles during immune responses. We have recently reported that platelets migrate at sites of infection in vitro and in vivo. Importantly, platelets use their ability to migrate to
Filip Bartnicki et al.
Journal of chromatography. A, 1418, 130-139 (2015-10-03)
Immobilized metal ion affinity chromatography (IMAC) is widely used for the purification of many different His6-tagged recombinant proteins. On the one hand, it is a powerful technique but on the other hand it has its disadvantages. In this report, we
Wenwu Yao et al.
Toxicon : official journal of the International Society on Toxinology, 100, 46-52 (2015-04-29)
Epsilon toxin (ETX) is produced by Clostridium perfringens type B and D strains, and is the causative agent of a lethal enterotoxemia in livestock animals and possibly in humans. However, many details of ETX structure and activity are not known.
Ana Blanco-Toribio et al.
AMB Express, 5(1), 137-137 (2015-08-05)
Recombinant antibodies are used with great success in many different diagnostic and therapeutic applications. A variety of protein expression systems are available, but nowadays almost all therapeutic antibodies are produced in mammalian cell lines due to their complex structure and
Mareike Bongers et al.
eLife, 9 (2020-03-13)
Volatile isoprenoids produced by plants are emitted in vast quantities into the atmosphere, with substantial effects on global carbon cycling. Yet, the molecular mechanisms regulating the balance between volatile and non-volatile isoprenoid production remain unknown. Isoprenoids are synthesised via sequential
X Guo et al.
Free radical research, 49(3), 228-235 (2014-12-10)
Gastrointestinal glutathione peroxidase (GI-GPx, GPx2) is a selenium-dependent enzyme and regarded as the first line of defense against oxidative stress caused by ingested pro-oxidants or gut microbes. As the essential part of the catalytic site of GPx2, selenocysteine (Sec) is
Gisele Espinha et al.
Molecular and cellular biochemistry, 404(1-2), 281-297 (2015-03-12)
Rac1 GTPase controls essential cellular functions related to the cytoskeleton, such as motility and adhesion. Rac1 is overexpressed in many tumor cells, including breast cancers, where it is also involved in the proliferation and checkpoint control necessary for the cell's
Deepesh Nagarajan et al.
BMC biochemistry, 16, 18-18 (2015-08-13)
Computational protein design is a rapidly maturing field within structural biology, with the goal of designing proteins with custom structures and functions. Such proteins could find widespread medical and industrial applications. Here, we have adapted algorithms from the Rosetta software
N Singh et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 968, 105-111 (2014-04-08)
SIRT6 is a histone deacetylase that has been proposed as a potential therapeutic target for metabolic disorders and the prevention of age-associated diseases. Thus the identification of compounds that modulate SIRT6 activity could be of great therapeutic importance. We have
Jessica R Ingram et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(36), E4975-E4984 (2015-08-26)
Calcium-dependent protein kinases (CDPKs) comprise the major group of Ca2+-regulated kinases in plants and protists. It has long been assumed that CDPKs are activated, like other Ca2+-regulated kinases, by derepression of the kinase domain (KD). However, we found that removal
Expression, purification and identification of Pla a1 in a codon-optimized Platanus pollen allergen.
Yun Liu et al.
Molecular medicine reports, 12(2), 2197-2202 (2015-04-23)
The present study aimed to express, purify and identify the major allergen gene, Pla a1, in Platanus pollen. According to previous studies, the major gene sequences of the Pla a1 allergen were obtained and codon optimization and synthesis of the
Xinran Li et al.
Genomics, proteomics & bioinformatics, 20(1), 87-100 (2021-09-24)
Proximity labeling catalyzed by promiscuous enzymes, such as APEX2, has emerged as a powerful approach to characterize multiprotein complexes and protein-protein interactions. However, current methods depend on the expression of exogenous fusion proteins and cannot be applied to identify proteins
Maritta Küspert et al.
RNA (New York, N.Y.), 21(7), 1294-1305 (2015-05-24)
mRNAs are key molecules in gene expression and subject to diverse regulatory events. Regulation is accomplished by distinct sets of trans-acting factors that interact with mRNAs and form defined mRNA-protein complexes (mRNPs). The resulting "mRNP code" determines the fate of
Pengkai Sun et al.
Nature communications, 13(1), 6562-6562 (2022-11-06)
Itaconate is a newly discovered endogenous metabolite promoting an anti-inflammatory program during innate immune response, but the precise mechanisms underlying its effect remains poorly understood owing primarily to the limitations of available itaconate-monitoring techniques. Here, we develop and validate a
Shuang Dou et al.
Advanced healthcare materials, 3(11), 1792-1803 (2014-06-21)
Patients with Her2-overexpressing (Her2(+)) breast cancers generally have a poorer prognosis due to the high aggressiveness and chemoresistance of the disease. Small interfering RNA (siRNA) targeting the gene encoding polo-like kinase 1 (Plk1; siPlk1) has emerged as an efficient therapeutic
Simone Hanna Brütsch et al.
Antioxidants & redox signaling, 22(4), 281-293 (2014-10-15)
Glutathione peroxidases (Gpx) and lipoxygenases (Alox) are functional counterplayers in the metabolism of hydroperoxy lipids that regulate cellular redox homeostasis. Gpx4 is a moonlighting protein that has been implicated not only as an enzyme in anti-oxidative defense, gene expression regulation
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