L4777

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关键词:'L4777'

显示 1-30 共 155 条结果关于 "L4777" 范围论文

The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

Daniela S Gerhard et al.

Genome research, 14(10B), 2121-2127 (2004-10-19)

The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random

Molecular cloning of a human gastric lipase and expression of the enzyme in yeast.

M W Bodmer et al.

Biochimica et biophysica acta, 909(3), 237-244 (1987-08-25)

The molecular cloning of a cDNA coding for human gastric lipase and its expression in yeast is described. A lipase present in human gastric aspirates was purified and its N-terminal amino-acid sequence was determined. This was found to be homologous

Preparation of magnetic Fe3O4@SiO2 nanoparticles for immobilization of lipase.

Wei Liu et al.

Journal of nanoscience and nanotechnology, 14(4), 3068-3072 (2014-04-17)

Magnetic Fe3O4 nanoparticles were prepared with coprecipitatation method and covered with SiO2 to form the core-shell Fe3O4@SiO2 nanoparticles. Then the nanoparticles were modified with glutaradehyde for functionalization of the surface to aldehyde groups. The transmission electron microscopy confirmed the core-shell

Screening of solvents for favoring hydrolytic activity of Candida antarctica Lipase B.

Bartłomiej Zieniuk et al.

Bioprocess and biosystems engineering, 43(4), 605-613 (2019-11-18)

Lipases are a group of enzymes of considerable significance in organic synthesis, among which Candida antarctica lipase B (CALB) is one of the most widely studied enzymes. The activity of the biocatalyst has been intensively characterized in many organic media

Isolation and cDNA sequence of human postheparin plasma hepatic triglyceride lipase.

G A Martin et al.

The Journal of biological chemistry, 263(22), 10907-10914 (1988-08-05)

Hepatic triglyceride lipase (H-TGL) was isolated from human postheparin plasma by column chromatography on heparin-Sepharose and phenyl-Sepharose and immunoaffinity chromatography with monoclonal antibodies. The purified enzyme had an apparent molecular weight of 65,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and

Cloning of a unique lipase from endothelial cells extends the lipase gene family.

K Hirata et al.

The Journal of biological chemistry, 274(20), 14170-14175 (1999-05-13)

A new lipoprotein lipase-like gene has been cloned from endothelial cells through a subtraction methodology aimed at characterizing genes that are expressed with in vitro differentiation of this cell type. The conceptual endothelial cell-derived lipase protein contains 500 amino acids

Facilitating chemical and biochemical experiments with electronic microcontrollers and single-board computers.

Gurpur Rakesh D Prabhu et al.

Nature protocols, 15(3), 925-990 (2020-01-31)

Since the advent of modern science, researchers have had to rely on their technical skills or the support of specialized workshops to construct analytical instruments. The notion of the 'fourth industrial revolution' promotes construction of customized systems by individuals using

A common variant in PNPLA3, which encodes adiponutrin, is associated with liver fat content in humans.

A Kotronen et al.

Diabetologia, 52(6), 1056-1060 (2009-02-19)

It has recently been suggested that the rs738409 G allele in PNPLA3, which encodes adiponutrin, is strongly associated with increased liver fat content in three different ethnic groups. The aims of the present study were as follows: (1) to try

Isolation and characterization of the human hepatic lipase gene.

D Ameis et al.

The Journal of biological chemistry, 265(12), 6552-6555 (1990-04-25)

Overlapping bacterial phage and cosmid genomic clones were isolated spanning an area of approximately 60 kilobases that contains the human hepatic lipase (HL) gene. It is composed of 9 exons spanning approximately 35 kilobases of DNA. The entire coding regions

Association of extreme blood lipid profile phenotypic variation with 11 reverse cholesterol transport genes and 10 non-genetic cardiovascular disease risk factors.

Alfredo Morabia et al.

Human molecular genetics, 12(21), 2733-2743 (2003-09-11)

This study explored the genetic basis of the combination of extreme blood levels of HDL-C and LDL-C, a well-studied endophenotype for CVD, which has several attractive features as a target for genetic analysis: (1) the trait is moderately heritable; (2)

Extended use of a selective inhibitor of acid lipase for the diagnosis of Wolman disease and cholesteryl ester storage disease.

G Civallero et al.

Gene, 539(1), 154-156 (2014-02-11)

Lysosomal acid lipase (LAL) deficiency produces two well defined inborn disorders, Wolman disease (WD) and cholesteryl ester storage disease (CESD). WD is a severe, early-onset condition involving massive storage of triglycerides and cholesteryl esters in the liver, with death usually

High-fat diet-mediated lipotoxicity and insulin resistance is related to impaired lipase expression in mouse skeletal muscle.

Pierre-Marie Badin et al.

Endocrinology, 154(4), 1444-1453 (2013-03-09)

Elevated expression/activity of adipose triglyceride lipase (ATGL) and/or reduced activity of hormone-sensitive lipase (HSL) in skeletal muscle are causally linked to insulin resistance in vitro. We investigated here the effect of high-fat feeding on skeletal muscle lipolytic proteins, lipotoxicity, and

The DNA sequence of the human X chromosome.

Mark T Ross et al.

Nature, 434(7031), 325-337 (2005-03-18)

The human X chromosome has a unique biology that was shaped by its evolution as the sex chromosome shared by males and females. We have determined 99.3% of the euchromatic sequence of the X chromosome. Our analysis illustrates the autosomal

[Nutritional factors of inflammation induction or lipid mechanism of intestinal endotoxin transport].

P L Okorokov et al.

Fiziologiia cheloveka, 38(6), 105-112 (2013-02-12)

Food behavior disorderes (high fat diet) and systematic strong drinks use could be the most important causes of excess intake of intestinal endotoxin (ET) in circulation (endotoxin aggression development) and induction of inflammation. In this work we investigated antiendotoxin immunity

Lipidemic responses of male broiler chickens to enzyme-supplemented wheat-soybean meal-based diets with various levels of metabolizable energy.

K Karimi et al.

Pakistan journal of biological sciences : PJBS, 16(21), 1295-1302 (2014-02-12)

Effects of 2 various levels of AME (according to the manual recommendation and 100 kcal kg(-1) less than it), 2 levels of endo-beta-D-mannanase enzyme (0, 1 g kg(-1)) and 2 levels of xylanase enzyme (0 and 1 g kg(-1)) on

Human hepatic lipase mutations and polymorphisms.

R A Hegele et al.

Human mutation, 1(4), 320-324 (1992-01-01)

Human hepatic lipase (HL) is a 477 residue glycoprotein that hydrolyzes triglycerides from plasma lipoproteins. Familial HL deficiency is a rare recessive disorder that is characterized by premature atherosclerosis and abnormal circulating lipoproteins. While studying the HL gene from the

Fat mobilization in adipose tissue is promoted by adipose triglyceride lipase.

Robert Zimmermann et al.

Science (New York, N.Y.), 306(5700), 1383-1386 (2004-11-20)

Mobilization of fatty acids from triglyceride stores in adipose tissue requires lipolytic enzymes. Dysfunctional lipolysis affects energy homeostasis and may contribute to the pathogenesis of obesity and insulin resistance. Until now, hormone-sensitive lipase (HSL) was the only enzyme known to

Two novel human pancreatic lipase related proteins, hPLRP1 and hPLRP2. Differences in colipase dependence and in lipase activity.

T Giller et al.

The Journal of biological chemistry, 267(23), 16509-16516 (1992-08-15)

We have isolated cDNAs coding for two novel human pancreatic lipase (hPL)-related human proteins, referred to as hPL-related proteins 1 and 2 (hPLRP1 and hPLRP2) and for hPL. The two novel proteins show an amino acid sequence identity to hPL

Characterization of the lipolytic activity of endothelial lipase.

Mary G McCoy et al.

Journal of lipid research, 43(6), 921-929 (2002-05-29)

Endothelial lipase (EL) is a new member of the triglyceride lipase gene family previously reported to have phospholipase activity. Using radiolabeled lipid substrates, we characterized the lipolytic activity of this enzyme in comparison to lipoprotein lipase (LPL) and hepatic lipase

Endothelial lipase modulates pressure overload-induced heart failure through alternative pathway for fatty acid uptake.

Hideto Nakajima et al.

Hypertension (Dallas, Tex. : 1979), 61(5), 1002-1007 (2013-03-06)

Lipoprotein lipase has been considered as the only enzyme capable of generating lipid-derived fatty acids for cardiac energy. Endothelial lipase is another member of the triglyceride lipase family and hydrolyzes high-density lipoproteins. Although endothelial lipase is expressed in the heart

Tissue expression pattern and polymorphism of G0S2 gene in porcine.

Yanzhi Jiang et al.

Gene, 539(1), 173-179 (2014-02-04)

Adipose triglyceride lipase (ATGL), catalyzing the initial step of hydrolysis of triacylglycerol (TAG) in adipocytes, has been known to be inhibited by G0/G1 switch protein 2 (G0S2). In this study, we determined tissue expression pattern and polymorphism of G0S2 gene

Covalent Incorporation of Trehalose within Hydrogels for Enhanced Long-Term Functional Stability and Controlled Release of Biomacromolecules.

Timothy M O'Shea et al.

Advanced healthcare materials, 4(12), 1802-1812 (2015-06-20)

Hydrogels with covalently incorporated trehalose are synthesized using thiol-ene Michael addition. Trehalose hydrogels afford prolonged stabilization and -controlled release of model enzymes in vitro and in vivo as well as preservation of protein stability under heat and -lyophilization stressors. Strong

Asymmetric Synthesis of Substituted Thiolanes through Domino Thia-Michael-Henry Dynamic Covalent Systemic Resolution using Lipase Catalysis.

Yan Zhang et al.

Advanced synthesis & catalysis, 356(5), 987-992 (2014-03-24)

Dynamic systems based on consecutive thia-Michael and Henry reactions were generated and transformed using lipase-catalyzed asymmetric transformation. Substituted thiolane structures with three contiguous stereocenters were resolved in the process in high yields and high enantiomeric excesses.

In vitro inhibitory effects of Limonium contortirameum and L. virgatum extracts from sardinia on alpha-amylase, alpha-glucosidase and pancreatic lipase.

Marzia Foddai et al.

Natural product communications, 9(2), 181-184 (2014-04-03)

Pancreatic triacylglycerol lipase (PL), alpha-amylase and alpha-glucosidase are interesting pharmacological targets for the management of dyslipidemia, atherosclerosis, and obesity-diabetes. Limonium spp (Plumbaginaceae) are endemic to Sardinia, Italy. Comparable with acarbose, aqueous extracts (AE) of L. contortirameum and L. virgatum, and

The DNA sequence of human chromosome 22.

I Dunham et al.

Nature, 402(6761), 489-495 (1999-12-11)

Knowledge of the complete genomic DNA sequence of an organism allows a systematic approach to defining its genetic components. The genomic sequence provides access to the complete structures of all genes, including those without known function, their control elements, and

Biocatalyzed Synthesis of Flavor Esters and Polyesters: A Design of Experiments (DoE) Approach.

Filippo Fabbri et al.

International journal of molecular sciences, 22(16) (2021-08-28)

In the present work, different hydrolases were adsorbed onto polypropylene beads to investigate their activity both in short-esters and polyesters synthesis. The software MODDE® Pro 13 (Sartorius) was used to develop a full-factorial design of experiments (DoE) to analyse the

Properties and purification of adipose tissue lipase.

W S LYNN et al.

The Journal of biological chemistry, 235, 1912-1916 (1960-07-01)

Human milk bile-salt stimulated lipase. Sequence similarity with rat lysophospholipase and homology with the active site region of cholinesterases.

D L Christie et al.

FEBS letters, 278(2), 190-194 (1991-01-28)

To determine the active site residue, human milk bile-salt stimulated lipase (BSSL) was labelled with [3H]diisopropyl fluorophosphate (DFP). Partial sequence analysis of cyanogen bromide fragments (a total of 146 residues from 6 peptides) revealed 84% sequence identity with a putative

Saturated fat-rich diet increases fetal lipids and modulates LPL and leptin receptor expression in rat placentas.

M B Mazzucco et al.

The Journal of endocrinology, 217(3), 303-315 (2013-03-14)

Metabolic alterations in obese and overweight mothers impact the placenta and the fetus, leading to anomalies in fetal growth and lipid accretion. The primary aim of the study was to examine the effect of a saturated fat-rich diet (FD) on

Human liver arylacetamide deacetylase. Molecular cloning of a novel esterase involved in the metabolic activation of arylamine carcinogens with high sequence similarity to hormone-sensitive lipase.

M R Probst et al.

The Journal of biological chemistry, 269(34), 21650-21656 (1994-08-26)

Microsomal arylacetamide deacetylase (DAC) competes against the activity of cytosolic arylamine N-acetyltransferase, which catalyzes one of the initial biotransformation pathways for arylamine and heterocyclic amine carcinogens in many species and tissues. Activity determination and immunoblot analysis of DAC in human

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