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Henry G Spratt et al.
Physiotherapy theory and practice, 30(7), 507-511 (2014-04-01)
A procedure commonly used in physical therapy (PT) clinics is therapeutic ultrasound (US). This equipment and associated gel comes in contact with patient skin, potentially serving as a reservoir for bacteria. In this study, we sampled US heads, gel bottle
Boyu Cui et al.
PloS one, 9(10), e107885-e107885 (2014-10-02)
The application of the luxCDABE operon of the bioluminescent bacterium Photorhabdus luminescens as a reporter has been published for bacteria, yeast and mammalian cells. We report here the optimization of fused luxAB (the bacterial luciferase heterodimeric enzyme) expression, quantum yield
Kimberly J Hassett et al.
Journal of pharmaceutical sciences, 104(2), 627-639 (2015-01-13)
During transport and storage, vaccines may be exposed to temperatures outside of the range recommended for storage, potentially causing efficacy losses. To better understand and prevent such losses, dominant negative inhibitor (DNI), a recombinant protein antigen for a candidate vaccine
Mercè Corominas et al.
International archives of allergy and immunology, 165(4), 244-246 (2015-01-31)
Immediate adverse reactions to glatiramer acetate (GA), a drug used in the treatment of patients with multiple sclerosis (MS), have been poorly investigated. We studied 3 MS patients who presented adverse reactions following GA administration. Two of them experienced severe
Chinwe Duru et al.
Biologicals : journal of the International Association of Biological Standardization, 43(2), 110-116 (2015-01-24)
Lyophilized Influenza antigen reference reagents are a critical resource in the quality control of influenza vaccines. A standard formulation has been used successfully at NIBSC for many years however, following the unexpected occurrence of a collapsed appearance in a particular
Jun Jiang et al.
Journal of virology, 89(13), 6695-6710 (2015-04-17)
Positive-sense RNA viruses remodel host cell endomembranes to generate quasi-organelles known as "viral factories" to coordinate diverse viral processes, such as genome translation and replication. It is also becoming clear that enclosing viral RNA (vRNA) complexes within membranous structures is
Oluwatomide Adeoye et al.
Acta poloniae pharmaceutica, 71(5), 803-811 (2014-11-05)
The study evaluated two novel coprocessed excipients (with two methods) as disintegrants in an orally disintegrating paracetamol tablet formulation. The tablets produced were assessed for mechanical properties with the use of friability and tensile strength while the release properties were
Li-Xia Qin et al.
Plant molecular biology, 86(6), 609-625 (2014-09-15)
Di19 (drought-induced protein19) family is a novel type of Cys2/His2 zinc-finger proteins. In this study, Arabidopsis Di19-3 was functionally characterized. The experimental results revealed that AtDi19-3 is a transcriptional activator, and could bind to the TACA(A/G)T sequence. AtDi19-3 expression in
Hermine V Mkrtchyan et al.
BMC microbiology, 15, 120-120 (2015-06-14)
Staphylococci are commonly associated with antibiotic resistance in healthcare settings including resistance to methicillin and other beta lactam antibiotics primarily associated with the carriage of SCCmec mobile genetic elements. We investigated oxacillin susceptibility in 11 different species of environmental staphylococci
K J Bär et al.
Neurobiology of aging, 24(2), 333-338 (2002-12-25)
Increasing evidence suggests an interaction of oxidative stress and the formation of advanced glycation end products (AGE) in the onset and progression of Alzheimer's disease. We studied levels of pentosidine and N(epsilon)-(carboxymethyl)-lysine (CML) in serum and cerebrospinal fluid (CSF) of
Peter L Pontoppidan et al.
International immunopharmacology, 25(1), 180-188 (2015-01-24)
Allogeneic hematopoietic stem cell transplantation (HSCT) is a procedure with a high risk of treatment related mortality. The primary aim of the present study was to examine associations between markers of gastrointestinal toxicity, markers of systemic inflammation, and plasma levels
Juerg Barben et al.
Pediatric pulmonology, 36(6), 490-494 (2003-11-18)
Our objective was to determine the repeatability of bronchial responsiveness to mannitol dry powder (MDP) as an objective marker of asthma in children. MDP challenge was performed in children with stable asthma at the same time of the day on
Simonetta Visintin et al.
International journal of food microbiology, 216, 69-78 (2015-10-02)
Yeast, lactic acid bacteria (LAB) and acetic acid bacteria (AAB) populations, isolated from cocoa bean heap and box fermentations in West Africa, have been investigated. The fermentation dynamicswere determined by viable counts, and 106 yeasts, 105 LAB and 82 AAB
Beth D Kirkpatrick et al.
The American journal of tropical medicine and hygiene, 92(4), 744-751 (2015-02-26)
Oral vaccines appear less effective in children in the developing world. Proposed biologic reasons include concurrent enteric infections, malnutrition, breast milk interference, and environmental enteropathy (EE). Rigorous study design and careful data management are essential to begin to understand this
Lene Myhren et al.
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 88(1), 186-193 (2014-04-22)
Despite recent advances in chemotherapy against acute myeloid leukaemia (AML), the disease still has high mortality, particularly for patients who tolerate extensive chemotherapy poorly. Nano-formulations have potential to minimise the adverse effects of chemotherapy. We present here a liposomal formulation
Hjalte Trnka et al.
Journal of pharmaceutical sciences, 103(9), 2839-2846 (2014-03-26)
Evaluation of freeze-dried biopharmaceutical formulations requires careful analysis of multiple quality attributes. The aim of this study was to evaluate the use of near-infrared (NIR) imaging for fast analysis of water content and related physical properties in freeze-dried formulations. Model
Intestinal permeability in subjects from two different race groups with diverse stone-risk profiles.
Takalani Theka et al.
Urolithiasis, 41(2), 111-117 (2013-03-19)
It is well established that calcium oxalate stones may be caused by colonic or ileum oxalate (Ox) hyperabsorption (secondary to intestinal dysfunction). Studies have reported that increased intestinal permeability (IP) can cause hyperabsorption of nutrients culminating in passive diffusion of
Xiangli Bai et al.
Autophagy, 16(6), 1111-1129 (2019-08-27)
Diabetes is a recognized high-risk factor for the development of atherosclerosis, in which macroautophagy/autophagy is emerging to play essential roles. The retention of low-density lipoprotein (LDL) particles in subendothelial space following transcytosis across the endothelium is the initial step of
Sattar Mohammadi et al.
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases, 25, 152-158 (2014-06-10)
Methicillin-resistant Staphylococcus aureus (MRSA) is a pathogen of public health importance. The prevalence of MRSA and its antimicrobial resistance pattern, as well as SCCmec and spa types, remain unclear both in the community and in the hospitals of the western
Kenia de Carvalho et al.
Molecular genetics and genomics : MGG, 289(5), 951-963 (2014-05-28)
Polyploid plants can exhibit transcriptional modulation in homeologous genes in response to abiotic stresses. Coffea arabica, an allotetraploid, accounts for 75% of the world's coffee production. Extreme temperatures, salinity and drought limit crop productivity, which includes coffee plants. Mannitol is
Nicholas F Marko
Critical care (London, England), 16(1), 113-113 (2012-02-23)
Hyperosmolar therapy is the principal medical management strategy for elevated intracranial pressure. Mannitol has been the primary hyperosmolar agent for nearly a century and remains the de facto gold standard for medical management of intracranial hypertension. Over the past 25
Mayu Kamimura et al.
Bioscience, biotechnology, and biochemistry, 78(3), 438-447 (2014-07-19)
Protein kinases are key regulators of cell function that constitute one of the largest and most functionally diverse gene families. We developed a novel assay system, based on the bimolecular fluorescence complementation (BiFC) technique in Escherichia coli, for detecting transient
Matthias Erber et al.
International journal of pharmaceutics, 495(2), 692-700 (2015-09-24)
A cryopellet formulation of the diagnostic protein ecarin has been developed that is suitable for use to monitor blood coagulation via in vitro thromboelastometry. The coagulation activity of the ecarin was measured by thromelastometry using whole blood. In aqueous solution
Kim Tardif et al.
Cardiovascular diabetology, 13, 119-119 (2014-08-21)
Nestin was reported to directly contribute to cell proliferation and the intermediate filament protein was detected in vascular smooth muscle cells. In experimental type I diabetes, nestin downregulation in the heart was identified as an incipient pathophysiological event. The following
Nermeen Adel ElKasabgy et al.
International journal of pharmaceutics, 472(1-2), 369-379 (2014-07-01)
Lipotomes were designed to enhance lacidipine's oral bioavailability by improving its solubility and enhancing the oral lymphatic uptake. Lipotomes were prepared using cetyl alcohol and Tween(®) 80 using a thin film hydration technique. Cetyl alcohol was chosen for imparting a
Enoch Chan et al.
Bioorganic & medicinal chemistry, 15(12), 4091-4097 (2007-04-11)
In search of new oligodeoxynucleotide (ODN) delivery agents, we evaluated novel peptides derived from core peptide H-GLRILLLKV-OH (CP). CP is a fragment designed from the T-cell antigen receptor (TCR) alpha-chain transmembrane sequence. CP was able to enter cells including T-cells
Congwu He et al.
The New phytologist, 206(3), 1051-1062 (2015-01-24)
Silicon (Si) plays a large number of diverse roles in plants, but the structural and chemical mechanisms operating at the single-cell level remain unclear. We isolate the cell walls from suspension-cultured individual cells of rice (Oryza sativa) and fractionate them
Dana M LeMoine et al.
Journal of the American Association for Laboratory Animal Science : JAALAS, 54(3), 311-316 (2015-06-06)
Aseptic technique includes the use of sterile surgical gloves for survival surgeries in rodents to minimize the incidence of infections. Exam gloves are much less expensive than are surgical gloves and may represent a cost-effective, readily available option for use
Zhenlong Cheng et al.
PloS one, 10(3), e0122450-e0122450 (2015-04-01)
Potato common scab is an economically important crop disease that is characterized by the formation of superficial, raised or pitted lesions on the potato tuber surface. The most widely distributed causative agent of the disease is Streptomyces scabies, which produces
Daniela Karadzovska et al.
Cutaneous and ocular toxicology, 32(4), 283-289 (2013-04-18)
The membrane-coated fiber (MCF) array technique was previously demonstrated to be a rapid, quantitative assessment of the percutaneous absorption of volatile compounds, capable of studying chemical mixtures and their synergistic effects. In particular, three commercially available fiber coatings (polydimethylsiloxane, polyacrylate
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