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关键词:'M9272'
显示 31-60 共 108 条结果 关于 "M9272" 范围 论文
Yi Deng et al.
Analytica chimica acta, 868, 45-52 (2015-03-31)
Rapid and ultrasensitive detection of trace heavy metal mercury(II) ions (Hg(2+)) are of significant importance due to the induced serious risks for environment and human health. This presented article reports the gold nanoparticle-based dual labeling colorimetric method (Dual-COLO) for ultrasensitive
Karol Kadlec et al.
Talanta, 147, 44-49 (2015-11-26)
The novel approach for hydroxyapatite (HA) surface characterization was proposed. The main aim of this investigation was to estimate surface properties of HA as a biomaterial in real system i.e. in simulated body fluid (SBF). One of the method, which
Laura M Wingler et al.
Cell, 176(3), 479-490 (2019-01-15)
The angiotensin II (AngII) type 1 receptor (AT1R) is a critical regulator of cardiovascular and renal function and is an important model for studies of G-protein-coupled receptor (GPCR) signaling. By stabilizing the receptor with a single-domain antibody fragment ("nanobody") discovered
Melina Schellhorn et al.
Oncotarget, 6(36), 39342-39356 (2015-10-30)
The antitumorigenic mechanism of the selective cyclooxygenase-2 (COX-2) inhibitor celecoxib is still a matter of debate. Using lung cancer cell lines (A549, H460) and metastatic cells derived from a lung cancer patient, the present study investigates the impact of celecoxib
Aaron L Albuck et al.
American journal of physiology. Heart and circulatory physiology, 320(2), H630-H641 (2020-11-10)
Peroxynitrite (PN), generated from the reaction of nitric oxide (NO) and superoxide, is implicated in the pathogenesis of ischemic and neurodegenerative brain injuries. Mitochondria produce NO from mitochondrial NO synthases and superoxide by the electron transport chain. Our objective was
Yusuke Tomina et al.
Bio-protocol, 8(5) (2018-03-30)
In this protocol, we introduce an effective method for voltage-sensitive dye (VSD) loading and imaging of leech ganglia as used in Tomina and Wagenaar (2017). Dissection and dye loading procedures are the most critical steps toward successful whole-ganglion VSD imaging.
Sang Hun Lee et al.
Neuron, 108(4), 676-690 (2020-09-07)
Amyloid precursor protein (APP) is associated with both familial and sporadic forms of Alzheimer's disease. Despite its importance, the role of APP family in neuronal function and survival remains unclear because of perinatal lethality exhibited by knockout mice lacking all
Hsien-Sung Huang et al.
PloS one, 9(5), e98383-e98383 (2014-05-27)
Genomic imprinting describes an epigenetic process through which genes can be expressed in a parent-of-origin-specific manner. The monoallelic expression of imprinted genes renders them particularly susceptible to disease causing mutations. A large proportion of imprinted genes are expressed in the
Iva Stojkovska et al.
STAR protocols, 2(1), 100372-100372 (2021-03-19)
The accumulation of proteins into insoluble aggregates is a common feature of several neurodegenerative diseases. Aggregated α-synuclein is a major component of Lewy bodies that pathologically define Parkinson's disease (PD). Here, we present methods for the detection of pathogenic conformations
Franziska E Uhl et al.
The European respiratory journal, 46(4), 1150-1166 (2015-05-02)
Chronic obstructive pulmonary disease (COPD) is characterised by a progressive loss of lung tissue. Inducing repair processes within the adult diseased lung is of major interest and Wnt/β-catenin signalling represents a promising target for lung repair. However, the translation of
Ester Falconer et al.
Nature protocols, 5(7), 1362-1377 (2010-07-03)
Previously, assays for sister chromatid segregation patterns relied on incorporation of 5-bromo-2'-deoxyuridine (BrdU) and indirect methods to infer segregation patterns after two cell divisions. In this study, we describe a method to differentially label sister chromatids of mouse cells and
Anthony J Snyder et al.
Bio-protocol, 8(2) (2018-03-20)
Mammalian orthoreovirus (reovirus) utilizes pore forming peptides to penetrate host cell membranes. This step is essential for delivering its genome containing core particle during viral entry. This protocol describes an in vitro assay for measuring reovirus-induced pore formation.
Siva S V P Sakamuri et al.
GeroScience, 40(3), 347-356 (2018-06-04)
Mitochondria play a critical role in the cardiomyocyte physiology by generating majority of the ATP required for the contraction/relaxation through oxidative phosphorylation (OXPHOS). Aging is a major risk factor for cardiovascular diseases (CVD) and mitochondrial dysfunction has been proposed as
Christian Frezza et al.
Nature protocols, 2(2), 287-295 (2007-04-05)
Mitochondria participate in key metabolic reactions of the cell and regulate crucial signaling pathways including apoptosis. Although several approaches are available to study mitochondrial function in situ are available, investigating functional mitochondria that have been isolated from different tissues and
Seung-Mok Lee et al.
Bioprocess and biosystems engineering, 38(12), 2323-2330 (2015-09-06)
In this study, Mg-sericite was used as a flocculant to harvest freshwater microalgae Chlorella vulgaris. Mg-sericite separated successfully >99% of the C. vulgaris at the following optimal parameters: sericite and MgCl2 ratio (S/M ratio) of 45 to 15, mixing time
Journal of AOAC International (2016-04-15)
Phytic acid, or myo-inositol hexakisphosphate, is the primary source of inositol and storage phosphorus in plant seeds and has considerable nutritional importance. In this form, phosphorus is unavailable for absorption by monogastric animals, and the strong chelating characteristic of phytic
Snx14 regulates neuronal excitability, promotes synaptic transmission, and is imprinted in the brain of mice.
Huang HS, Yoon BJ, Brooks S, et al.
PLoS ONE, 9(5), e98383-e98383 (2014)
Laura Baselga-Escudero et al.
PloS one, 8(7), e69817-e69817 (2013-08-08)
miR-33 and miR-122 are major regulators of lipid metabolism in the liver, and their deregulation has been linked to the development of metabolic diseases such as obesity and metabolic syndrome. However, the biological importance of these miRNAs has been defined
Hardik Chandasana et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 985, 180-188 (2015-02-16)
Apocynin is a major active constituent of Picrorhiza kurroa that exhibits potent anti-inflammatory activity by inhibiting superoxide-generating NADPH oxidase enzyme. To elucidate detailed pharmacokinetic profile of apocynin, high-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) method was developed in
Siavash Fazel Darbandi et al.
Cell reports, 31(2), 107495-107495 (2020-04-16)
Tbr1 is a high-confidence autism spectrum disorder (ASD) gene encoding a transcription factor with distinct pre- and postnatal functions. Postnatally, Tbr1 conditional knockout (CKO) mutants and constitutive heterozygotes have immature dendritic spines and reduced synaptic density. Tbr1 regulates expression of
Eloïse de Tredern et al.
Cell reports, 36(8), 109620-109620 (2021-08-26)
Brain function relies almost solely on glucose as an energy substrate. The main model of brain metabolism proposes that glucose is taken up and converted into lactate by astrocytes to fuel the energy-demanding neuronal activity underlying plasticity and memory. Whether
Dominic A Helmerich et al.
ACS nano, 14(10), 12629-12641 (2020-08-18)
The degree of labeling (DOL) of antibodies has so far been optimized for high brightness and specific and efficient binding. The influence of the DOL on the blinking performance of antibodies used in direct stochastic optical reconstruction microscopy (dSTORM) has
Ivana Savic Azoulay et al.
Journal of neurochemistry, 153(2), 203-215 (2020-01-25)
Acid-sensing ion channel 1a (ASIC1a) is well-known to play a major pathophysiological role during brain ischemia linked to acute acidosis of ~pH 6, whereas its function during physiological brain activity, linked to much milder pH changes, is still poorly understood.
Monica Mazzarino et al.
Journal of pharmaceutical and biomedical analysis, 117, 47-60 (2015-09-08)
This work presents an analytical method for the simultaneous analysis in human urine of 38 pharmacologically active compounds (19 benzodiazepine-like substances, 7 selective serotonin reuptake inhibitors, 4 azole antifungal drugs, 5 inhibitors of the phosphodiesterases type 4 and 3 inhibitors
Yun-Xia Zhu et al.
STAR protocols, 2(3), 100728-100728 (2021-08-20)
Pancreatic islet β cells secrete insulin in a biphasic manner when sensing high blood glucose level. This protocol describes the evaluation of different phases of insulin secretion, as well as basal, glucose-stimulated and total insulin secretion abilities, thereby enabling precise
Yashpal S Chhonker et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 965, 127-132 (2014-07-13)
Nonoxynol-9 (N-9), a microbicidal spermicide, has been in use as an over-the-counter contraceptive since the 1960s. A detailed account of its pharmacokinetic profile using highly sensitive detection method has not been reported yet. We developed and validated a rapid, selective
Ikuo Yamamiya et al.
Drug metabolism and disposition: the biological fate of chemicals, 42(9), 1485-1492 (2014-07-09)
Tegafur (FT), a prodrug of 5-fluorouracil, is a chiral molecule, a racemate of R- and S-isomers, and CYP2A6 plays an important role in the enantioselective metabolism of FT in human liver microsomes (R-FT > S-FT). This study examined the enantioselective
Modification of poly (vinyl alcohol) films by the addition of magnesium chloride hexahydrate.
Jiang X, et al.
Polym. Eng. Sci., 52(7), 1565-1570 (2012)
Jian-Feng Li et al.
Nature protocols, 9(4), 939-949 (2014-03-29)
Artificial miRNA (amiRNA) technology offers highly specific gene silencing in diverse plant species. The principal challenge in amiRNA application is to select potent amiRNAs from hundreds of bioinformatically designed candidates to enable maximal target gene silencing at the protein level.
Douglas M Fowler et al.
Nature protocols, 9(9), 2267-2284 (2014-08-29)
Deep mutational scanning marries selection for protein function to high-throughput DNA sequencing in order to quantify the activity of variants of a protein on a massive scale. First, an appropriate selection system for the protein function of interest is identified
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