room temperature.
Procedures
TLC Spray
The developed TLC plate is first dried in a stream of
warm air and then sprayed evenly with the spray
reagent until the silica layer begins to become
transparent
Tarantini, et al., J. of Molecular Catalysis B: Enzymatic 11, 2001, 343.
70 TLC, on precoated Silica Gel plates with fluorescent indicator 254 nm (No 99573, 09916).
Eluent: AcOEt/MeOH/H2O (8:4:
can be
found in Table 2, Table 3, Figure 3 and Figure 4.
All TLC analyses were performed using HPTLC Silica gel
60 F254. The plates were pre-washed with the mobile
phase (up to 7 cm) and dried before
purification is done by HPLC on silica gel
allowing no buffer salts to be used for the purification.
The recommended TLC spray reagent for detection on
silica gel plates is the naphthoresorcinol-sulfuric
purification is done by HPLC on silica gel
allowing no buffer salts to be used for the purification.
The recommended TLC spray reagent for detection on
silica gel plates is the naphthoresorcinol-sulfuric
purification is done by HPLC on silica gel
allowing no buffer salts to be used for the purification.
The recommended TLC spray reagent for detection on
silica gel plates is the naphthoresorcinol-sulfuric
p-hydroxybenzoate and
methyl-hydroxybenzoate, which as the TLC plate shows, is a
difficult separation. We loaded twice as much sample on the
23 g VersaPak cartridge compared to the 40 g competitive
Chromolith® columns are made from a single piece of high-purity poly-
meric silica gel and are not packed with small silica particles. This novel
technology achieves a very high separation performance
solution (acetone,
chlorinated solvents, hexanes). In this case, the sorbents
are usually silica or modified silica (CN, NH2, or Diols)
that enable either hydrogen bonding, dipole-dipole, or
dipole-dipole-induced
the mobile phase.
e = Vm/Vempty
tm = Vempty e/fv
For totally porous materials like silica and modified silica, e is between 0.7
and 0.8. The void time may also be determined by measuring the retention
properly stored in a
solution. This hydrolysis could be readily detected on
assay by TLC on a silica gel plate using
chloroform:methanol:water (65:25:4, v/v/v). The dried
or powder material stored
Description
Chromolith® columns are made from a single piece of high-purity
polymeric silica gel and are not packed with small silica particles.
This new technology achieves a very high separation performance
along
culture is harvested by
centrifugation and subjected to a modified alkaline-SDS
lysis procedure followed by adsorption of the DNA onto
silica in the presence of high salts.1,2 Contaminants are
then
culture is harvested by
centrifugation and subjected to a modified alkaline-SDS
lysis procedure followed by adsorption of the DNA onto
silica in the presence of high salts.1,2 Contaminants are
then
Discovery® silica specifications, see page 2.
For general guidelines on normal-phase SPE, see page 51.
DSC-Si • Unbonded acid washed silica sorbent ideal for normal-phase SPE and other modified flash techniques
of your column.
Monolithic silica
Chromolith® Widepore columns are made from a single piece of high-purity
polymeric silica gel and are not packed with small silica particles. This new
technology
protocols. The columns are based on high-purity silica;
hence they minimize the negative effect of trace metals. Furthermore, the silica rods are chemically
modified with n-alkyl chains that possess a high ligand
corrects for any background
absorbance, including that caused by silica fines in the
final product. These fines are common with silica-based
systems and will have no effect on downstream
applications
corrects for any background
absorbance, including that caused by silica fines in the
final product. These fines are common with silica-based
systems and will have no effect on downstream
applications
culture is harvested by
centrifugation and subjected to a modified alkaline-SDS
lysis procedure followed by adsorption of the DNA onto
silica in the presence of high salts.1,2 Contaminants are
then removed
corrects for any background
absorbance, including that caused by silica fines in the
final product. These fines are common with silica-based
systems and will have no effect on downstream
applications
alcohol precipitation.
These kits feature a filter syringe for rapidly clearing the
lysate, and a silica-binding column that can be used with
a vacuum or spin purification format. The result is high qual
advantages of silica binding
with a convenient spin column format, eliminating the
need for expensive resins or toxic organic compounds
such as phenol and chloroform.
DNA is bound on a silica membrane within
optimized if needed.
For larger experimental setup, we recommend using microtiter plates with the “Duetz” sandwich cover
plate system from EnzyScreen BV (Heemstede, Netherlands) together with a humidity-controlled