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Carla S Vizzotto et al.
Genome announcements, 6(22) (2018-06-02)
We report the whole-genome sequence of Muricauda sp. strain K001 isolated from a marine cyanobacterial culture. This genome sequence will improve our understanding of the influence of heterotrophic bacteria on the physiology of cyanobacteria and may contribute to the development
Ákos Nyerges et al.
Nucleic acids research, 42(8), e62-e62 (2014-02-07)
Oligonucleotide-mediated multiplex genome engineering is an important tool for bacterial genome editing. The efficient application of this technique requires the inactivation of the endogenous methyl-directed mismatch repair system that in turn leads to a drastically elevated genomic mutation rate and
Semen A Leyn et al.
Microbial genomics, 7(5) (2021-05-05)
Understanding the dynamics and mechanisms of acquired drug resistance across major classes of antibiotics and bacterial pathogens is of critical importance for the optimization of current anti-infective therapies and the development of novel ones. To systematically address this challenge, we
Subhajit Basu et al.
Microbial ecology, 65(4), 934-954 (2013-01-03)
A massive algal bloom of the dinoflagellate Noctiluca miliaris (green) was located in the Northern Arabian Sea by IRS-P4-2 (OCM-II) for microbiological studies, during two consecutive cruises of February-March 2009. Culturable bacterial load during bloom were ≈ 2-3-fold higher in
Joyce Megumi Sakamoto et al.
Insects, 11(10) (2020-10-24)
Native microbiota represent a potential resource for biocontrol of arthropod vectors. Ixodes scapularis is mostly inhabited by the endosymbiotic Rickettsia buchneri, but the composition of bacterial communities varies with life stage, fed status, and/or geographic location. We compared bacterial community
Manuel Simões et al.
Journal of basic microbiology, 47(3), 230-242 (2007-05-24)
Biocides generally have multiple biochemical targets. Such a feature easily entangles the analysis of the mechanisms of antimicrobial action. In this study, the action of the dialdehyde biocide ortho-phtalaldehyde (OPA), on bacteria, was investigated using the Gram-negative Pseudomonas fluorescens. The
Jaime E Zlamal et al.
mBio, 12(3), e0098721-e0098721 (2021-06-23)
Resistance to the broad-spectrum antibiotic ciprofloxacin is detected at high rates for a wide range of bacterial pathogens. To investigate the dynamics of ciprofloxacin resistance development, we applied a comparative resistomics workflow for three clinically relevant species of Gram-negative bacteria:
Anna I Lauxen et al.
Pharmaceuticals (Basel, Switzerland), 14(5) (2021-05-01)
During the last decades, a continuous rise of multi-drug resistant pathogens has threatened antibiotic efficacy. To tackle this key challenge, novel antimicrobial therapies are needed with increased specificity for the site of infection. Photopharmacology could enable such specificity by allowing
Patrycja Golinska et al.
Antonie van Leeuwenhoek, 103(5), 1079-1088 (2013-02-02)
Actinomycetes growing on acidified starch-casein agar seeded with suspensions of litter and mineral soil from a spruce forest were provisionally assigned to the genus Nocardia based upon colonial properties. Representative isolates were found to grow optimally at pH 5.5, have
Changhan Lee et al.
Genome announcements, 2(2) (2014-03-22)
Pseudomonas aeruginosa SG17M is an environmental isolate recovered from river water in the city of Mulheim, Germany. SG17M belongs to clone C, which is distributed worldwide. This is the first clone C strain whose genome sequence has been determined.
Courtney E Chandler et al.
Journal of bacteriology, 201(5) (2018-12-12)
Pseudomonas aeruginosa is an opportunistic pathogen found ubiquitously in the environment and commonly associated with airway infection in patients with cystic fibrosis. P. aeruginosa strain PAO1 is one of the most commonly used laboratory-adapted research strains and is a standard
Maria Georgieva et al.
iScience, 24(8), 102923-102923 (2021-08-26)
Antibiotic resistance is an increasing threat for public health, underscoring the need for new antibacterial agents. Antimicrobial peptides (AMPs) represent an alternative to classical antibiotics. TAT-RasGAP317-326 is a recently described AMP effective against a broad range of bacteria, but little
Erin M Gaffney et al.
Microbiology resource announcements, 9(43) (2020-10-24)
The halophilic bacterium Salinivibrio sp. strain EAGSL was isolated from the Great Salt Lake (Utah) for use in microbial electrochemical technologies experiencing fluctuating salt concentrations. Genome sequencing was performed with Ion Torrent technology, and the assembled genome reported here is
Pablo Emiliano Tomatis et al.
Scientific reports, 9(1), 2483-2483 (2019-02-23)
Eukaryotic integral membrane proteins (IMPs) are difficult to study due to low functional expression levels. To investigate factors for efficient biogenesis of eukaryotic IMPs in the prokaryotic model organism Escherichia coli, important, e.g., for isotope-labeling for NMR, we selected for
David Greenwood et al.
Proteomics. Clinical applications, 14(3), e2000011-e2000011 (2020-03-31)
Periodontitis is linked to a localized dysbiotic microbial shift. This trending may often not be evident due to deep taxonomic changes of low abundance organisms and lack of consideration of variations in the treatment response. By using next generation sequencing
Steffen Porwollik et al.
Methods in molecular biology (Clifton, N.J.), 394, 89-103 (2008-03-28)
Microarray technology provides a convenient and relatively inexpensive way of investigating the genetic content of bacterial genomes by comparative genomic hybridization. In this method, genomic DNA of an unknown bacterial strain of interest and that of a closely related sequenced
Alba Pacheco-Moreno et al.
eLife, 10 (2021-11-19)
Agricultural soil harbors a diverse microbiome that can form beneficial relationships with plants, including the inhibition of plant pathogens. Pseudomonas spp. are one of the most abundant bacterial genera in the soil and rhizosphere and play important roles in promoting
Elena Espinosa et al.
STAR protocols, 1(3), 100202-100202 (2020-12-31)
Sister chromatid interactions are a key step to ensure the successful segregation of sister chromatids after replication. Our knowledge about this phenomenon is mostly based on microscopy approaches, which have some constraints such as resolution limit and the impossibility of
V V Kothari et al.
Genome announcements, 1(5) (2013-09-07)
Bacillus safensis strain VK was isolated from the rhizosphere of a cumin plant growing in the saline desert of Radhanpar, Gujarat, India. Here, we provide the 3.68-Mb draft genome sequence of B. safensis VK, which might provide information about the
Jianbo Zhang et al.
Med (New York, N.Y.), 2(1), 74-98 (2021-01-30)
The gut microbiome plays an important role in human health and disease. Gnotobiotic animal and in vitro cell-based models provide some informative insights into mechanistic crosstalk. However, there is no existing system for a long-term co-culture of a human colonic
H R Shehata et al.
Journal of applied microbiology, 120(3), 756-769 (2016-01-09)
The aim of this study was to determine if endophytes from wild and ancient Zea plants (corn family) have anti-fungal activities, specifically against the most important fungal pathogen (Sclerotinia homoeocarpa) of creeping bentgrass, a relative of Zea, used here as
Kylie Standage-Beier et al.
ACS synthetic biology, 4(11), 1217-1225 (2015-10-10)
Programmable CRISPR-Cas systems have augmented our ability to produce precise genome manipulations. Here we demonstrate and characterize the ability of CRISPR-Cas derived nickases to direct targeted recombination of both small and large genomic regions flanked by repetitive elements in Escherichia
Ruoxi Chen et al.
PloS one, 7(12), e52150-e52150 (2013-01-04)
Burkholderia glumae causes bacterial panicle blight of rice and produces major virulence factors, including toxoflavin, under the control of the quorum-sensing (QS) system mediated by the luxI homolog, tofI, and the luxR homolog, tofR. In this study, a series of
David B Adimpong et al.
Genome announcements, 1(2), e0009713-e0009713 (2013-03-30)
The Bacillus sonorensis L12 draft genome sequence is approximately 4,647,754 bp in size with a G+C content of 45.2%. Over 86% of the genome contains protein-encoding genes, including several gene clusters for de novo biosynthesis of the nonribosomal lipopeptides iturin, bacitracin
Benoy Jacob et al.
International journal of dentistry, 2021, 5563945-5563945 (2021-09-14)
The aim of the present study was to compare the antibacterial effectiveness of chlorhexidine and PPE oral rinse on S. mutans, Lactobacilli, and Veillonella, in clinical salivary samples of patients with advanced stages of dental caries at baseline and two
Andrew I Perault et al.
Cell host & microbe, 28(4), 534-547 (2020-08-07)
Pseudomonas aeruginosa and Burkholderia cepacia complex (Bcc) species are opportunistic lung pathogens of cystic fibrosis (CF) patients. While P. aeruginosa can initiate long-term infections in younger CF patients, Bcc infections only arise in teenagers and adults. Both P. aeruginosa and Bcc use
Ákos Nyerges et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(9), 2502-2507 (2016-02-18)
Currently available tools for multiplex bacterial genome engineering are optimized for a few laboratory model strains, demand extensive prior modification of the host strain, and lead to the accumulation of numerous off-target modifications. Building on prior development of multiplex automated
Tristan Cerisy et al.
Journal of bacteriology, 201(15) (2019-05-22)
The mechanisms by which bacteria uptake solutes across the cell membrane broadly impact their cellular energetics. Here, we use functional genomic, genetic, and biophysical approaches to reveal how Clostridium (Lachnoclostridium) phytofermentans, a model bacterium that ferments lignocellulosic biomass, uptakes plant
Klaus Gori et al.
Microbial ecology, 65(3), 602-615 (2012-12-12)
For studying the microbiota of four Danish surface-ripened cheeses produced at three farmhouses and one industrial dairy, both a culture-dependent and culture-independent approach were used. After dereplication of the initial set of 433 isolates by (GTG)5-PCR fingerprinting, 217 bacterial and
Vikash Chandra Tripathi et al.
Free radical research, 54(2-3), 150-161 (2020-02-06)
The marine environment is emerging as a biodiversity resource for the discovery of natural molecules or chemical scaffolds with pharmaceutical activity. Marine microbes have a tremendous ability to sense and respond to their surroundings to survive in a stressful environment
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