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Elena Espinosa et al.
STAR protocols, 1(3), 100202-100202 (2020-12-31)
Sister chromatid interactions are a key step to ensure the successful segregation of sister chromatids after replication. Our knowledge about this phenomenon is mostly based on microscopy approaches, which have some constraints such as resolution limit and the impossibility of
V V Kothari et al.
Genome announcements, 1(5) (2013-09-07)
Bacillus safensis strain VK was isolated from the rhizosphere of a cumin plant growing in the saline desert of Radhanpar, Gujarat, India. Here, we provide the 3.68-Mb draft genome sequence of B. safensis VK, which might provide information about the
Jianbo Zhang et al.
Med (New York, N.Y.), 2(1), 74-98 (2021-01-30)
The gut microbiome plays an important role in human health and disease. Gnotobiotic animal and in vitro cell-based models provide some informative insights into mechanistic crosstalk. However, there is no existing system for a long-term co-culture of a human colonic
David Greenwood et al.
Proteomics. Clinical applications, 14(3), e2000011-e2000011 (2020-03-31)
Periodontitis is linked to a localized dysbiotic microbial shift. This trending may often not be evident due to deep taxonomic changes of low abundance organisms and lack of consideration of variations in the treatment response. By using next generation sequencing
Alba Pacheco-Moreno et al.
eLife, 10 (2021-11-19)
Agricultural soil harbors a diverse microbiome that can form beneficial relationships with plants, including the inhibition of plant pathogens. Pseudomonas spp. are one of the most abundant bacterial genera in the soil and rhizosphere and play important roles in promoting
Steffen Porwollik et al.
Methods in molecular biology (Clifton, N.J.), 394, 89-103 (2008-03-28)
Microarray technology provides a convenient and relatively inexpensive way of investigating the genetic content of bacterial genomes by comparative genomic hybridization. In this method, genomic DNA of an unknown bacterial strain of interest and that of a closely related sequenced
Pablo Emiliano Tomatis et al.
Scientific reports, 9(1), 2483-2483 (2019-02-23)
Eukaryotic integral membrane proteins (IMPs) are difficult to study due to low functional expression levels. To investigate factors for efficient biogenesis of eukaryotic IMPs in the prokaryotic model organism Escherichia coli, important, e.g., for isotope-labeling for NMR, we selected for
Joyce Megumi Sakamoto et al.
Insects, 11(10) (2020-10-24)
Native microbiota represent a potential resource for biocontrol of arthropod vectors. Ixodes scapularis is mostly inhabited by the endosymbiotic Rickettsia buchneri, but the composition of bacterial communities varies with life stage, fed status, and/or geographic location. We compared bacterial community
Subhajit Basu et al.
Microbial ecology, 65(4), 934-954 (2013-01-03)
A massive algal bloom of the dinoflagellate Noctiluca miliaris (green) was located in the Northern Arabian Sea by IRS-P4-2 (OCM-II) for microbiological studies, during two consecutive cruises of February-March 2009. Culturable bacterial load during bloom were ≈ 2-3-fold higher in
Manuel Simões et al.
Journal of basic microbiology, 47(3), 230-242 (2007-05-24)
Biocides generally have multiple biochemical targets. Such a feature easily entangles the analysis of the mechanisms of antimicrobial action. In this study, the action of the dialdehyde biocide ortho-phtalaldehyde (OPA), on bacteria, was investigated using the Gram-negative Pseudomonas fluorescens. The
Ákos Nyerges et al.
Nucleic acids research, 42(8), e62-e62 (2014-02-07)
Oligonucleotide-mediated multiplex genome engineering is an important tool for bacterial genome editing. The efficient application of this technique requires the inactivation of the endogenous methyl-directed mismatch repair system that in turn leads to a drastically elevated genomic mutation rate and
Anna I Lauxen et al.
Pharmaceuticals (Basel, Switzerland), 14(5) (2021-05-01)
During the last decades, a continuous rise of multi-drug resistant pathogens has threatened antibiotic efficacy. To tackle this key challenge, novel antimicrobial therapies are needed with increased specificity for the site of infection. Photopharmacology could enable such specificity by allowing
Patrycja Golinska et al.
Antonie van Leeuwenhoek, 103(5), 1079-1088 (2013-02-02)
Actinomycetes growing on acidified starch-casein agar seeded with suspensions of litter and mineral soil from a spruce forest were provisionally assigned to the genus Nocardia based upon colonial properties. Representative isolates were found to grow optimally at pH 5.5, have
Maria Georgieva et al.
iScience, 24(8), 102923-102923 (2021-08-26)
Antibiotic resistance is an increasing threat for public health, underscoring the need for new antibacterial agents. Antimicrobial peptides (AMPs) represent an alternative to classical antibiotics. TAT-RasGAP317-326 is a recently described AMP effective against a broad range of bacteria, but little
Courtney E Chandler et al.
Journal of bacteriology, 201(5) (2018-12-12)
Pseudomonas aeruginosa is an opportunistic pathogen found ubiquitously in the environment and commonly associated with airway infection in patients with cystic fibrosis. P. aeruginosa strain PAO1 is one of the most commonly used laboratory-adapted research strains and is a standard
Erin M Gaffney et al.
Microbiology resource announcements, 9(43) (2020-10-24)
The halophilic bacterium Salinivibrio sp. strain EAGSL was isolated from the Great Salt Lake (Utah) for use in microbial electrochemical technologies experiencing fluctuating salt concentrations. Genome sequencing was performed with Ion Torrent technology, and the assembled genome reported here is
Changhan Lee et al.
Genome announcements, 2(2) (2014-03-22)
Pseudomonas aeruginosa SG17M is an environmental isolate recovered from river water in the city of Mulheim, Germany. SG17M belongs to clone C, which is distributed worldwide. This is the first clone C strain whose genome sequence has been determined.
Carla S Vizzotto et al.
Genome announcements, 6(22) (2018-06-02)
We report the whole-genome sequence of Muricauda sp. strain K001 isolated from a marine cyanobacterial culture. This genome sequence will improve our understanding of the influence of heterotrophic bacteria on the physiology of cyanobacteria and may contribute to the development
Semen A Leyn et al.
Microbial genomics, 7(5) (2021-05-05)
Understanding the dynamics and mechanisms of acquired drug resistance across major classes of antibiotics and bacterial pathogens is of critical importance for the optimization of current anti-infective therapies and the development of novel ones. To systematically address this challenge, we
Jaime E Zlamal et al.
mBio, 12(3), e0098721-e0098721 (2021-06-23)
Resistance to the broad-spectrum antibiotic ciprofloxacin is detected at high rates for a wide range of bacterial pathogens. To investigate the dynamics of ciprofloxacin resistance development, we applied a comparative resistomics workflow for three clinically relevant species of Gram-negative bacteria:
Sarah Paszti et al.
International journal of molecular sciences, 23(9) (2022-05-15)
Burkholderia cenocepacia is an opportunistic pathogen that can lead to severe infections in patients suffering from cystic fibrosis (CF) and chronic granulomatous disease. Being an obligate aerobe, B. cenocepacia is unable to grow in the absence of oxygen. In this
Tristan Cerisy et al.
Applied and environmental microbiology, 83(11) (2017-04-02)
Increasing the resistance of plant-fermenting bacteria to lignocellulosic inhibitors is useful to understand microbial adaptation and to develop candidate strains for consolidated bioprocessing. Here, we study and improve inhibitor resistance in Clostridium phytofermentans (also called Lachnoclostridium phytofermentans), a model anaerobe
Iram Liaqat et al.
Current microbiology, 68(3), 342-351 (2013-10-30)
The quorum sensing (QS) dependent behaviour of micro-organisms, in particular expression of virulence genes, biofilm formation and dispersal, have provided impetus for investigating practical approaches to interfere with microbial QS. This study tests Halomonas pacifica and Marinobacter hydrocarbonoclasticus, two halophilic
Sonalli Ganguly et al.
Genome announcements, 4(3) (2016-06-11)
Here, we report the draft genome sequence of Dietzia maris, known previously as Rhodococcus maris It is 3,505,372 bp in size with a G+C content of 73%. The draft genome sequence will improve our understanding of Dietzia maris related to other
Mu Peng et al.
Microbiology spectrum, 10(1), e0259121-e0259121 (2022-02-03)
Rhodanobacter species dominate in the Oak Ridge Reservation (ORR) subsurface environments contaminated with acids, nitrate, metal radionuclides, and other heavy metals. To uncover the genomic features underlying adaptations to these mixed-waste environments and to guide genetic tool development, we sequenced
Aoife J McHugh et al.
NPJ science of food, 5(1), 4-4 (2021-02-17)
Efficient and accurate identification of microorganisms throughout the food chain can potentially allow the identification of sources of contamination and the timely implementation of control measures. High throughput DNA sequencing represents a potential means through which microbial monitoring can be
Kai Bao et al.
Virulence, 6(3), 265-273 (2015-01-15)
Periodontal infection involves a complex interplay between oral biofilms, gingival tissues and cells of the immune system in a dynamic microenvironment. A humanized in vitro model that reduces the need for experimental animal models, while recapitulating key biological events in
Vikash Chandra Tripathi et al.
Free radical research, 54(2-3), 150-161 (2020-02-06)
The marine environment is emerging as a biodiversity resource for the discovery of natural molecules or chemical scaffolds with pharmaceutical activity. Marine microbes have a tremendous ability to sense and respond to their surroundings to survive in a stressful environment
Sophia Belkhelfa et al.
Frontiers in microbiology, 10, 1313-1313 (2019-07-10)
The bio-economy relies on microbial strains optimized for efficient large scale production of chemicals and fuels from inexpensive and renewable feedstocks under industrial conditions. The reduced one carbon compound methanol, whose production does not involve carbohydrates needed for the feed
Brandon Istain et al.
Microbiology resource announcements, 10(10) (2021-03-13)
Here, we present the draft genome sequence (∼4.7 Mb) of the endopyhtic bacterium Pantoea agglomerans strain R6, which was isolated from surface-sterilized roots of Lactuca serriola (prickly lettuce).
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