Storage
Seal tightly and protect from moisture (seal the plastic bag well after
removing Anaerocult® C mini).
Recommended storage temperature: +15 °C �� + 25 °C.
1
Insert one or two Petri dishes in
(Fig. 4)
5
the Petri dish with the
attached
immediately into the special
incubation bag and with
(Cat. No. 1.14255.)
seal with an heat
(it is advisable to seal with a
double
PF10623 Rev A Nutrient Petri-Pad Petri Dish User Guide.indd
Nutrient Petri-Pad™ Petri Dish User Guide
Introduction
The Nutrient Petri-Pad Dish combines the advantages
of simple membrane
® C mini
25 special incubation bags
Anaerotest® 1.32371.0001 50 strips
Petri-dish rack 1.07040.0001 For up to 12 petri dishes
To place an order or receive technical
assistance
Order/Customer Service
P
25 special incubation bags
Anaerotest® 1.32371.0001 50 test stripes
Petri-dish rack 1.07040.0001 For up to 12 petri dishes
Anaeroclip® 1.14226.0001 25 Anaeroclip®
To place an order or receive
mini
25 special incubation bags
Anaerotest® 1.32371.0001 50 test stripes
Petri-dish rack 1.07040.0001 For up to 12 petri dishes
Anaeroclip® 1.14226.0001 25 Anaeroclip®
To place an order or receive
pump MCLHEAD01
Petri Dishes
47 mm Petri Dishes, packed in sleeves of 25 dishes. Available with or without cellulose pad.
Description (1/Pk) Qty/Pk 100 mL Funnels Cat. No.
Petri Dish without pad
pump MCLHEAD01
Petri Dishes
47 mm Petri Dishes, packed in sleeves of 25 dishes. Available with or without cellulose pad.
Description (1/Pk) Qty/Pk 100 mL Funnels Cat. No.
Petri Dish without pad
pump MCLHEAD01
Petri Dishes
47 mm Petri Dishes, packed in sleeves of 25 dishes. Available with or without cellulose pad.
Description (1/Pk) Qty/Pk 100 mL Funnels Cat. No.
Petri Dish without pad
1.32381.0001 10 Anaerocult® A
Anaerotest® 1.32371.0001 50 test stripes
Petri-dish rack 1.07040.0001 For up to 12 petri dishes
Anaerobic jar 1.13681.0001 2,5 L-volume
To place an order or receive
filter.
11. Secure the lid on the petri dish and invert the dish.
12. Incubate the inverted petri dish for 48-72 hours at 25 - 35° C.
13. Remove the petri dish from the
six-well tissue culture plates, these
inserts are usually used individually in standard 35 or 50 mm
petri dishes to reduce the possibility of contamination.
NOTE: When working with Millicell inserts, use
smooth-tip forceps, transfer the filter to a clean petri dish for visual particle counting, or to
a prepared media pad or agar plate in a sterile petri dish for microbial contaminant culturing.
20225294
Place in clean Petri dish or desiccator.
2. Backfill Petri dish with dry nitrogen.
3. For long-term storage - Place Petri dishes in a
jar backfilled with dry N2 and sealed with
Parafilm.
solidification to obtain a dry surface.
After inoculation, change to a Brewer anaerobic petri plate cover. The sealing ring inside the cover should make a
perfect contact with the medium and should not be
completely. Sterilize by autoclaving at
121°C for 15 minutes. Cool to 50°C and pour into sterile petri plates.
Principle and Interpretation:
Bacillus coagulans is commonly found in soil and has been
.
Care should be taken to prevent any scratching of the
sealing surfaces on the head and on the sampler.
Figure 2: Standard 90 mm Petri dish on a MAS-100 NT® microbial air
sampler. The impaction
tightly-sealed containers at 2-25°C.
Appearance: Yellow colored, homogeneous, free flowing powder.
Gelling: Firm
Color and Clarity: Yellow colored, clear to slightly opalescent gel forms in Petri plates
bubbles are not trapped below the filter.
10. Secure the lid on the petri dish and invert the dish.
11. Incubate the inverted petri dish for 24 - 72 hours at 25-35° C. Note: 35° C
filter.
10. Secure the lid on the petri dish and invert the dish.
11. Incubate the inverted petri dish for 48-72 hours at 30-35° C.
12. Remove the petri dish from the incubator. Use
filter.
10. Secure the lid on the petri dish and invert the dish.
11. Incubate the inverted petri dish for 5-7 days at 28-32° C.
12. Remove the petri dish from the incubator. Use
filter.
10. Secure the lid on the petri dish and invert the dish.
11. Incubate the inverted petri dish for 5-7 days at 28-32° C.
12. Remove the petri dish from the incubator. Use
filter.
10. Secure the lid on the petri dish and invert the dish.
11. Incubate the inverted petri dish for 24 - 48 hours at 35° C.
12. Remove the petri dish from the
. Secure the lid on the petri dish and invert the dish.
11. Incubate the inverted petri dish for 24 - 48 hours (48 hours is recommended) at 35 +/- 0.5° C.
12. Remove the petri dish from the incubator.
the filter.
10. Secure the lid on the petri dish and invert the dish.
11. Incubate the inverted petri dish for 3-5 days at 30° C.
12. Remove the petri dish from the incubator.
shake. Pour 15 ml of the nutrient
medium into each Petri dish.
Storage of the ready-to-use test agar
The ready-to-use test agar can be sealed into Petri dishes with airtight
adhesive tape and stored in
the
addition of 15% v/v sterile defibrinated blood, a cherry red colored, opaque gel
forms in petri plates.
Directions:
Suspend 30 g of Bordet Gengou Agar Base without Peptone in 1000 ml of distilled
the
addition of 15% v/v sterile defibrinated blood, a cherry red colored, opaque gel
forms in petri plates.
Directions:
Suspend 40 g of Bordet Gengou Agar Base in 1000 ml of distilled water containing
tightly-sealed containers at 2-25°C.
Appearance: Yellow colored, homogeneous, free flowing powder.
Gelling: Firm
Color and Clarity: Yellow colored, clear to slightly opalescent gel forms in petri plates
filter.
10. Secure the lid on the petri dish and invert the dish.
11. Incubate the inverted petri dish for 24 +/- 2 hours at 35 +/- 0.5° C.
12. Remove the petri dish