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Pramod J Kasabe et al.
Protein expression and purification, 116, 30-41 (2015-08-16)
The novel bacterium, Rhodococcus sp. PKPD-CL was isolated and identified from the 'Chilika Lake' located at Odisha state of India, which is a largest brackish water habitat in Asia. Rhodococcus sp. PKPD-CL produces extracellular halo tolerant, detergent and organic solvent
Nina S Liland et al.
Lipids, 53(7), 737-747 (2018-09-28)
Several feeding trials with Atlantic salmon fed naturally high phytosterol concentrations due to dietary rapeseed oil inclusion have shown changes in lipid metabolism and increased hepatic lipid storage in the fish. An in vitro trial with Atlantic salmon hepatocytes was
Wendy R Sorenson et al.
Journal of AOAC International, 89(1), 22-34 (2006-03-04)
In conjunction with an AOAC Presidential Task Force on Dietary Supplements, a method was validated for measurement of 3 plant sterols (phytosterols) in saw palmetto raw materials, extracts, and dietary supplements. AOAC Official Method 994.10, "Cholesterol in Foods," was modified
Ki Hyun Kim et al.
Bioscience, biotechnology, and biochemistry, 76(4), 825-827 (2012-04-10)
A new steroid, itesmol 3-O-palmitate (1), along with two known steroids were isolated from the trunk of Berberis koreana. The structure of 1 was determined on the basis of spectroscopic analyses involving 2D NMR and chemical reactions. Compound 1 exhibited
Azadeh Hamedi et al.
Journal of natural medicines, 69(3), 387-396 (2015-04-11)
Pharmacognostic evaluation of medicinal plants may assess their current applications and possibly results in finding new active components. In this study, ash and extractive values and high performance thin layer chromatography fingerprints of Alyssum homolocarpum (Brassicaceae) seed extracts were investigated
Thomas Griebel et al.
The Plant journal : for cell and molecular biology, 63(2), 254-268 (2010-05-07)
Upon inoculation with pathogenic microbes, plants induce an array of metabolic changes that potentially contribute to induced resistance or even enhance susceptibility. When analysing leaf lipid composition during the Arabidopsis thaliana-Pseudomonas syringae interaction, we found that accumulation of the phytosterol
Hyocheol Bae et al.
Pharmaceutics, 12(6) (2020-06-03)
Phytosterols have physiological effects and are used as medicines or food supplements. Stigmasterol has shown anticancer effects against various cancers such as hepatoma, cholangiocarcinoma, gall bladder carcinoma, endometrial adenocarcinoma and skin, gastric, breast, prostate, and cervical cancer. However, there are
Zahra Izadi et al.
Journal of food science and technology, 52(8), 5341-5346 (2015-08-06)
Phytosterols enriched products are innovative types of functional foods, in which dairy products, like low fat yogurt are ideal vehicles for this functional component. In this study, phytosterol dispersions were prepared using an oil/water (O/W) emulsion. The emulsion was added
Verena K Burg et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 33(41), 16072-16087 (2013-10-11)
Amyloid-β (Aβ), major constituent of senile plaques in Alzheimer's disease (AD), is generated by proteolytic processing of the amyloid precursor protein (APP) by β- and γ-secretase. Several lipids, especially cholesterol, are associated with AD. Phytosterols are naturally occurring cholesterol plant
Poliana Guerino Marson Ascêncio et al.
Evidence-based complementary and alternative medicine : eCAM, 2014, 190543-190543 (2015-01-15)
Costus spiralis (Costaceae) is a species native to the Amazon region and is used in traditional medicine. The endophytic fungi used in this study were obtained from leaves of this plant. 13 strains were selected to obtain hydroethanolic extracts and
Andrei Mocan et al.
International journal of molecular sciences, 16(9), 21109-21127 (2015-09-26)
Aerial parts of Veronica species are used in Romanian traditional medicine for the treatment of various conditions like kidney diseases, cough, and catarrh, and are known for their wound-healing properties. In the present study, the phenolic and sterolic content and
Poonamjot Deol et al.
Endocrinology, 161(2) (2020-01-09)
Soybean oil consumption has increased greatly in the past half-century and is linked to obesity and diabetes. To test the hypothesis that soybean oil diet alters hypothalamic gene expression in conjunction with metabolic phenotype, we performed RNA sequencing analysis using
Sara Hoet et al.
Journal of natural products, 70(8), 1360-1363 (2007-07-20)
Fractionation of an antitrypanosomal lipophilic leaf extract from Strychnos spinosa led to the isolation of eight triterpenoids and sterols in this plant part for the first time. Two of these were found to possess in vitro antitrypanosomal activity, namely, saringosterol
Ting Shi et al.
Food chemistry, 287, 46-54 (2019-03-13)
Proton nuclear magnetic resonance (1H NMR) combined with partial least squares (PLS) was developed for the rapid determination of squalene and sterols (brassicasterol, campesterol, stigmasterol and β-sitosterol) in 119 vegetable oils from 7 different species. The 1H NMR spectra of
Jin-Song Liu et al.
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials, 35(8), 1246-1250 (2013-01-17)
To study the triterpenoids constituents from Wisteria sinensis Sweet Caulis. The compounds were beta-solated and purified with silica gel and Sephadex LH-20 column chromatography from the petroleum ether extract. Their structures were determined on the basis of physicochemical properties and
L Alemany et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 50(9), 3106-3113 (2012-07-04)
The biological implications of cholesterol oxidation products have been investigated, though research on plant sterol oxidation products is scarce and in some cases contradictory. The cytotoxicity of 7keto(k)-stigmasterol versus 7keto(k)-cholesterol at different concentrations (0-120 μM) and incubation times (4-24h), in
Blanca Barriuso et al.
Steroids, 99(Pt B), 155-160 (2015-02-24)
The characteristics of the lipid matrix surrounding sterols exert a great influence in their thermal oxidation process. The objective of this work was to assess the oxidation susceptibility of equal amounts of cholesterol and stigmasterol within a sunflower oil lipid
Norbert Vida et al.
Steroids, 77(12), 1212-1218 (2012-08-02)
Three new polyamine conjugates with stigmasterol [(3β,22E)-stigmasta-5,22-dien-3-ol] were synthesized and subjected to basic antimicrobial and cytotoxic tests. The conjugate derived from spermine, (3β,22E)-stigmasta-5,22-dien-3-yl 4(12-amino-4,9-diaza-dodecylamino)-4-oxobutanoate (5c), displayed considerable antimicrobial activity on Staphylococcus aureus at low concentration (50 μg mL(-1)). The cytotoxic
Hannah C Lifsey et al.
The Journal of nutritional biochemistry, 76, 108263-108263 (2019-11-24)
Despite advances in healthcare, cardiovascular disease (CVD) remains the leading cause of death in the United States. Elevated levels of plasma cholesterol are highly predictive of CVD and stroke and are the principal driver of atherosclerosis. Unfortunately, current cholesterol lowering
Aaron O Antwi et al.
International immunopharmacology, 53, 105-113 (2017-10-28)
Stigmasterol is a naturally occurring steroid alcohol which occurs in vegetables, soya and a large variety of medicinal plants. Stigmasterol and other phytosterols have been documented as immunomodulators with huge therapeutic potential. We assessed the mitigating effect of stigmasterol on
Fan C Wang et al.
Food & function, 8(11), 3964-3969 (2017-10-04)
Phytosterols and phytosterol esters were encapsulated within large unilamellar liposomes prepared with soy phospholipids using a microfluidizer. The average particle diameter of these liposomal vesicles increased with increasing amounts of encapsulated phytosterols, especially with increasing free sterol content. The phytosterol
Annika Mutanen et al.
The American journal of clinical nutrition, 100(4), 1085-1094 (2014-08-08)
Increased serum concentrations of plant sterols, including stigmasterol, during parenteral nutrition (PN) have been linked with serum biochemical signs of intestinal failure-associated liver disease (IFALD), whereas clinical data on their correlation to histologic liver injury have been limited. We studied
Aaron O Antwi et al.
BioMed research international, 2018, 3984068-3984068 (2018-08-25)
The therapeutic potential of stigmasterol, a natural steroid alcohol with established immune-modulatory properties, was assessed on allergic cutaneous responses. We examined its suppressive effect on immunoglobulin E (IgE)-mediated active cutaneous anaphylaxis (ACA), compound 48/80 (C48/80)-induced pruritus, and irritant dermatitis induced
Satyendra K Prasad et al.
The Journal of pharmacy and pharmacology, 66(12), 1808-1817 (2014-08-19)
To scientifically validate the traditional substitution of roots of highly expensive Aconitum heterophyllum (AH) with rhizomes of Cryptocoryne spiralis (CS) in the treatment of diarrhoea. Different fractions from root/rhizome extract of both the plant were subjected to faecal excretion rate
Flora Pensec et al.
Journal of agricultural and food chemistry, 62(32), 7998-8007 (2014-07-25)
Triterpenoids present in grape cuticular waxes are of interest due to their potential role in protection against biotic stresses, their impact on the mechanical toughness of the fruit surface, and the potential industrial application of these biologically active compounds from
Gregori Casals et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 960, 8-13 (2014-05-02)
Gas chromatography-mass spectrometry (GC-MS) remains as the gold-standard technique for the study of the steroid metabolome. A main limitation is the need of performing a derivatization step since incubation with strong silylations agents for long periods of time (usually 16
Phan Van Kiem et al.
Bioorganic & medicinal chemistry letters, 21(24), 7460-7465 (2011-11-11)
The rhizomes of Hedychium coronarium have been used for the treatment of inflammation, skin diseases, headache, and sharp pain due to rheumatism in traditional medicine. From this plant, three new labdane-type diterpenes 1-3, named coronarins G-I as well as seven
E M Nogovitsina et al.
Bioorganicheskaia khimiia, 37(5), 697-704 (2012-02-16)
The conditions for a directed biocatalytic oxidation of beta-sitosterol to a pharmacologically promising stigmast-4-en-3-one using Rhodococcus actinobacteria were selected. It was shown that palmitic acid induced the cholesterol oxidase reaction and allowed for the decrease in the bioconversion process duration
Luiz C Klein-Júnior et al.
Basic & clinical pharmacology & toxicology, 111(3), 145-153 (2012-03-03)
Polygala cyparissias, used in folk medicine as an anaesthetic, has already demonstrated antinociceptive activity against acute pain. In this study, we investigated the antihyperalgesic activity of the P. cyparissias methanol extract (PCME) from which the following compounds were isolated: α-spinasterol
Tomas J Poklepovich et al.
Steroids, 96, 132-139 (2015-02-15)
Tetrahymena thermophila transforms exogenous cholesterol into pro-vitamin D3 (7-dehydrocholesterol) with remarkable efficiency in a one-step reaction carried out by a C-7 cholesterol desaturase. The enzyme DES7 is encoded by the gene TTHERM_00310640, identified with RNAi and gene knock-out experiments, but
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