T4424

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Improvement of the plaque technique for human rotaviruses: effect of fetal bovine serum, acetyltrypsin and agar.

Y Imamura et al.

The Kurume medical journal, 38(4), 251-258 (1991-01-01)

Dilution of viruses, which were previously treated with 10 micrograms acetyltrypsin/ml, in MEM supplemented with 2.5% fetal bovine serum caused little damage of cell monolayers resulting in the formation of discrete plaques. When agar and acetyltrypsin were added to the

Increased proteolysis of collagen in an in vitro tensile overload tendon model.

Thomas L Willett et al.

Annals of biomedical engineering, 35(11), 1961-1972 (2007-09-04)

Presently, there is a lack of fundamental understanding regarding changes in collagen's molecular state due to mechanical damage. The bovine tail tendon (BTT; steers approximately 30 months) was characterized and used as an in vitro model for investigating the effect

A method for the isolation and culture of embryonic cardiomyocytes from Mexican axolotl.

R W Zajdel et al.

The International journal of developmental biology, 40(4), 907-908 (1996-08-01)

Role of pancreatic trypsin in chronic esophagitis induced by gastroduodenal reflux in rats.

Yuji Naito et al.

Journal of gastroenterology, 41(3), 198-208 (2006-05-16)

Reflux of the duodenal contents with gastric acid has been reported to contribute to the development of esophageal mucosal damage and inflammation. Recent studies show that pancreatic trypsin can stimulate the production of inflammatory mediators, including chemokines and prostaglandins from

Single-Cell Transcriptomics of Traced Epidermal and Hair Follicle Stem Cells Reveals Rapid Adaptations during Wound Healing.

Simon Joost et al.

Cell reports, 25(3), 585-597 (2018-10-18)

Epithelial tissues, such as the skin, rely on cellular plasticity of stem cells (SCs) from different niches to restore tissue function after injury. How these molecularly and functionally diverse SC populations respond to injury remains elusive. Here, we genetically labeled

Treatment of proton pump inhibitor-resistant patients with gastroesophageal reflux disease.

Yoshikazu Kinoshita et al.

Journal of gastroenterology, 41(3), 286-287 (2006-05-16)

Stem cell depletion in Hutchinson-Gilford progeria syndrome.

Ylva Rosengardten et al.

Aging cell, 10(6), 1011-1020 (2011-09-10)

Hutchinson-Gilford progeria syndrome (HGPS or progeria) is a very rare genetic disorder with clinical features suggestive of premature aging. Here, we show that induced expression of the most common HGPS mutation (LMNA c.1824C>T, p.G608G) results in a decreased epidermal population

Preservation of Functional Microvascular Bed Is Vital for Long-Term Survival of Cardiac Myocytes Within Large Transmural Post-Myocardial Infarction Scar.

Colleen Nofi et al.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 66(2), 99-120 (2017-11-09)

This study was aimed to understand the mechanism of persistent cardiac myocyte (CM) survival in myocardial infarction (MI) scars. A transmural MI was induced in 12-month-old Sprague-Dawley rats by permanent coronary artery ligation. The hearts were collected 3 days, 1

Oncogenic PIK3CA induces centrosome amplification and tolerance to genome doubling.

Inma M Berenjeno et al.

Nature communications, 8(1), 1773-1773 (2017-11-25)

Mutations in PIK3CA are very frequent in cancer and lead to sustained PI3K pathway activation. The impact of acute expression of mutant PIK3CA during early stages of malignancy is unknown. Using a mouse model to activate the Pik3ca H1047R hotspot

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