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Akihiro Ishii et al.
Nature communications, 5, 5651-5651 (2014-12-03)
Bats can carry important zoonotic pathogens. Here we use a combination of next-generation sequencing and classical virus isolation methods to identify novel nairoviruses from bats captured from a cave in Zambia. This nairovirus infection is highly prevalent among giant leaf-nosed
Christine Lin Chin et al.
BMC biotechnology, 15, 44-44 (2015-06-03)
Expression vector engineering technology is one of the most convenient and timely method for cell line development to meet the rising demand of novel production cell line with high productivity. Destabilization of dihydrofolate reductase (dhfr) selection marker by addition of
Eriko Simamura et al.
PloS one, 10(4), e0123232-e0123232 (2015-04-11)
In this study, we showed that adrenocorticotropic hormone (ACTH) promoted erythroblast differentiation and increased the enucleation ratio of erythroblasts. Because ACTH was contained in hematopoietic medium as contamination, the ratio decreased by the addition of anti-ACTH antibody (Ab). Addition of
Bo Bai et al.
Experimental cell research, 328(2), 401-409 (2014-09-07)
During our research on apelin receptor (APJ) signalling in living cells with BRET and FRET, we demonstrated that apelin-13 stimulation can lead to the activation of Gαi2 or Gαi3 through undergoing a molecular rearrangement rather than dissociation in HEK293 cells
Sébastien Sart et al.
Tissue engineering. Part C, Methods, 21(4), 356-366 (2014-09-05)
Neural progenitor cells are usually derived from pluripotent stem cells (PSCs) through the formation of embryoid bodies (EBs), the three-dimensional (3D) aggregate-like structure mimicking embryonic development. Cryo-banking of EBs is a critical step for sample storage, process monitoring, and preservation
Aaron Silva-Sanchez et al.
PloS one, 10(2), e0118171-e0118171 (2015-02-24)
Variability in the developing antibody repertoire is focused on the third complementarity determining region of the H chain (CDR-H3), which lies at the center of the antigen binding site where it often plays a decisive role in antigen binding. The
Bhairab Mondal et al.
Applied microbiology and biotechnology, 99(22), 9791-9803 (2015-08-22)
Aptamers are synthetic DNA recognition elements which form unique conformations that enable them to bind specifically to their targets. In the present study, an attempt was made to standardize a new modified combinatorial method comprising of Ni-NTA affinity Systematic Evolution
Magdalena Banas et al.
PloS one, 10(2), e0117830-e0117830 (2015-02-07)
Chemerin is a protein ligand for the G protein-coupled receptor CMKLR1 and also binds to two atypical heptahelical receptors, CCRL2 and GPR1. Chemerin is a leukocyte attractant, adipokine, and antimicrobial protein. Although chemerin was initially identified as a highly expressed
Luigino Grasso et al.
Analytical and bioanalytical chemistry, 407(18), 5425-5432 (2015-05-01)
We report on a generic method to detect and identify the molecular profile of exosomes either derived from cultured cell lines or isolated from biofluids. Exosomes are nanovesicles shed by cells into their microenvironment and carry the molecular identity of their
Hyo Jung Choi et al.
PloS one, 10(6), e0129761-e0129761 (2015-06-20)
The first identification of swine-originated influenza A/CA/04/2009 (pH1N1) as the cause of an outbreak of human influenza accelerated efforts to develop vaccines to prevent and control influenza viruses. The current norm in many countries is to prepare influenza vaccines using
Carla De Giovanni et al.
Breast cancer research : BCR, 16(1), R10-R10 (2014-01-24)
The availability of mice transgenic for the human HER2 gene (huHER2) and prone to the development of HER2-driven mammary carcinogenesis (referred to as FVB-huHER2 mice) prompted us to study active immunopreventive strategies targeting the human HER2 molecule in a tolerant
Fairoz Algaar et al.
Analytical chemistry, 87(16), 8394-8398 (2015-07-08)
Crimean-Congo hemorrhagic fever (CCHF) is a severe viral disease with high fatality rate. CCHF virus is endemic in parts of Africa, Asia, the Middle East, and southeastern Europe. Rapid diagnostics of CCHF is vital for appropriate clinical management and prevention
S Yao et al.
Mucosal immunology, 8(4), 746-759 (2014-12-04)
Respiratory syncytial virus (RSV) infection is a leading cause of severe lower respiratory tract illness in young infants, the elderly and immunocompromised individuals. We demonstrate here that the co-inhibitory molecule programmed cell death 1 (PD-1) is selectively upregulated on T
Wensheng Xie et al.
PloS one, 10(3), e0119738-e0119738 (2015-03-18)
Endoplasmic reticulum stress plays a critical role to restore the homeostasis of protein production in eukaryotic cells. This vital process is hence involved in many types of diseases including COPD. PERK, one branch in the ER stress signaling pathways, has
Rashmi Gupta et al.
Journal of translational medicine, 12, 256-256 (2014-09-17)
Psoriasis is a multifactorial, chronic disease of skin affecting 2-3% of the world's population. Genetic studies of psoriasis have identified a number of susceptibility genes that are involved in anti-viral immunity. Furthermore, physiological studies have also found an increase in
C Oliver et al.
Journal of applied microbiology, 119(2), 365-376 (2015-05-29)
To produce and characterize egg yolk immunoglobulin (IgY) against the fish intracellular pathogen Piscirickettsia salmonis as well as to evaluate the antibacterial activity of IgY in vitro and the availability in the serum of fish immunized orally. Specific IgY was
Michael Baldwin et al.
Biochimica et biophysica acta, 1843(12), 2855-2870 (2014-08-27)
Plasmodium falciparum takes advantage of two broadly defined alternate invasion pathways when infecting human erythrocytes: one that depends on and the other that is independent of host sialic acid residues on the erythrocyte surface. Within the sialic acid-dependent (SAD) and
Dong-Goo Kim et al.
International immunopharmacology, 26(1), 194-202 (2015-04-07)
Guggulsterone (GS), a plant steroid and a compound found at high levels in Commiphora myrrha, exhibits anti-inflammatory, anti-cancer, and cholesterol-lowering effects. However, the potential of GS to ameliorate acute pancreatitis (AP) is unknown. The aim of this study was to
Christine Y Y Wai et al.
PloS one, 9(11), e111649-e111649 (2014-11-05)
Designer proteins deprived of its IgE-binding reactivity are being sought as a regimen for allergen-specific immunotherapy. Although shrimp tropomyosin (Met e 1) has long been identified as the major shellfish allergen, no immunotherapy is currently available. In this study, we
Lionel Tortolano et al.
Journal of biomedical materials research. Part A, 103(12), 3843-3851 (2015-06-27)
The aim of this study is to investigate in vitro interactions between hydrophobic acrylate intraocular lenses (IOLs) and their biological environment. The influence of lens chemical composition and aging on fibronectin (FN) adsorption and on IOLs cytotoxicity on human lens
R Wierzbicka et al.
Journal of immunological methods, 413, 12-24 (2014-07-30)
Alkylresorcinols (ARs) are amphiphilic phenolic lipids and their two main metabolites, 3-(3,5-dihydroxyphenyl)-propanoic acid (DHPPA) and 3,5-dihydroxybenzoic acid (DHBA), can be used as biomarkers of whole grain wheat and rye intake. The aim of this study was to develop antibodies against
Ningyan Zhang et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 21(23), 5380-5390 (2015-08-01)
Studies have demonstrated that cancer-associated matrix metalloproteinases (MMP) can generate single peptide bond cleavages in the hinge region of immunoglobulin G1 (IgG1). This study investigated the cleavage of endogenous IgGs by MMPs in the tumor microenvironment and the consequences of
Randall J Smith et al.
Biomaterials, 51, 303-312 (2015-03-17)
We demonstrate the ability of immobilized vascular endothelial growth factor (VEGF) to capture endothelial cells (EC) with high specificity under fluid flow. To this end, we engineered a surface consisting of heparin bound to poly-l-lysine to permit immobilization of VEGF
Juozas Nainys et al.
BMC biotechnology, 14, 100-100 (2014-12-10)
Porcine circovirus type 2 (PCV2) is considered to be an important emerging pathogen associated with a number of different syndromes and diseases in pigs known as PCV2-associated diseases. It has been responsible for significant mortality among pigs and remains a
Pia Dosenovic et al.
Immunology and cell biology, 93(2), 136-146 (2014-10-15)
Signalling through Toll-like receptors (TLRs) by endogenous components of viruses or bacteria can promote antibody (Ab) isotype switching to IgG2a/c. Multiple cell types are capable of responding to TLR stimulation in vivo and the processes underlying TLR-induced Ab isotype switching
Mark Pimentel et al.
PloS one, 10(5), e0126438-e0126438 (2015-05-15)
Diarrhea-predominant irritable bowel syndrome (IBS) is diagnosed through clinical criteria after excluding "organic" conditions, and can be precipitated by acute gastroenteritis. Cytolethal distending toxin B (CdtB) is produced by bacteria that cause acute gastroenteritis, and a post-infectious animal model demonstrates
Yong Yang et al.
Parasitology, 142(6), 783-790 (2015-01-20)
Trichinella spiralis is an intracellular parasitic nematode of mammalian skeletal muscle, causing a serious zoonotic disease in humans and showing a high economic impact mainly in pig breeding. Serine proteinases of T. spiralis play important roles in the host-parasite interactions
Mark D Bunting et al.
Immunology and cell biology, 92(6), 489-498 (2014-03-19)
T-cell selection and development occurs as precursor cells journey through the thymus and interact with stromal cells residing in distinct microenvironments. Although the chemokines CCL19, CCL21, CCL25 and CXCL12 are known to have major roles in intrathymic migration of thymocytes
Sheng Wang et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 13(5), 1199-1204 (2012-03-03)
The origin of the peroxidase-like activity of gold nanoparticles and the impact of surface modification are studied. Furthermore, some influencing factors, such as fabrication process, redox property of the modifier, and charge property of the substrate, are investigated. Compared to
Jennifer A Hetz et al.
Molecular and cellular endocrinology, 411, 38-48 (2015-04-22)
Maturation of the mammalian growth axis is thought to be linked to the transition from fetal to post-natal life at birth. However, in an altricial marsupial, the tammar wallaby (Macropus eugenii), this process occurs many months after birth but at
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