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显示 1-30 共 183 条结果 关于 "T6567" 范围 论文
Kenichi Yamaguchi
Methods in molecular biology (Clifton, N.J.), 775, 241-264 (2011-08-25)
Proteomics of chloroplast ribosomes in spinach and Chlamydomonas revealed unique protein composition and structures of plastid ribosomes. These studies have suggested the presence of some ribosomal proteins unique to plastid ribosomes which may be involved in plastid-unique translation regulation. Considering
Lucia F Zacchi et al.
Journal of proteome research, 19(5), 2149-2158 (2020-03-26)
Proteomic analysis of bioreactor supernatants can inform on cellular metabolic status, viability, and productivity, as well as product quality, which can in turn help optimize bioreactor operation. Incubating mammalian cells in bioreactors requires the addition of polymeric surfactants such as
Ronivaldo Rodrigues da Silva et al.
Applied biochemistry and biotechnology, 187(4), 1158-1172 (2018-09-05)
The fungal genus Pyrenochaetopsis has received particular attention because of its different lifestyles, such as numerous plant pathogenic, saprophytic, and endophytic species. Its ability to infect plant cells relies heavily upon secreted peptidases. Here, we investigated the biochemical properties and
Dylan J Harney et al.
Molecular & cellular proteomics : MCP, 18(9), 1899-1915 (2019-07-17)
Unbiased and sensitive quantification of low abundance small proteins in human plasma (e.g. hormones, immune factors, metabolic regulators) remains an unmet need. These small protein factors are typically analyzed individually and using antibodies that can lack specificity. Mass spectrometry (MS)-based
Yan S Xu et al.
Scientific reports, 6, 39517-39517 (2016-12-23)
Cytoplasmic STAT3, after activation by growth factors, translocates to different subcellular compartments, including nuclei and mitochondria, where it carries out different biological functions. However, the precise mechanism by which STAT3 undergoes mitochondrial translocation and subsequently regulates the tricarboxylic acid (TCA)
Hui Zheng et al.
Molecules (Basel, Switzerland), 18(11), 13425-13433 (2013-11-02)
A novel chemoenzymatic one-pot multicomponent synthesis of thiazole derivatives was developed. A series of thiazole derivatives were synthesized with high yields up to 94% under mild enzyme-catalyzed conditions. The blank and control experiments reveal that trypsin from porcine pancreas (PPT)
Guillaume Bastin et al.
Frontiers in microbiology, 11, 1157-1157 (2020-06-26)
Pathogenic enteric viruses and bacteriophages such as Qβ and MS2 are transmitted through the fecal-oral route. However, oxidants such as peroxynitrite (ONOOH) and hypochlorous acid (HClO) can prevent new infection by inactivating infectious viruses. Their virucidal effect is well recognized
Gary R Klinefelter et al.
Reproduction (Cambridge, England), 144(6), 747-761 (2012-10-09)
Significant research has been focused on phthalate-induced alterations in male reproductive development. Studies on rodents have prompted the notion that a syndrome exists in the human male which includes phenotypic alterations such as hypospadias, cryptorchidism, poor semen quality, and even
Bin-Tong Yang et al.
Annals of the New York Academy of Sciences, 1486(1), 58-75 (2020-10-04)
Aeromonas veronii is an important zoonotic and aquatic pathogen. An increasing number of reports indicate that it has caused substantial economic losses in the aquaculture industry, in addition to threatening human health. However, little is known about its pathogenesis. Exploration
Jeonghoon Lee et al.
Journal of the American Society for Mass Spectrometry, 19(7), 964-972 (2008-05-16)
An automated proteolytic digestion bioreactor and droplet deposition system was constructed with a plastic microfluidic device for off-line interfacing to matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The microfluidic chips were fabricated in poly(methyl methacrylate) (PMMA), using a
Javier Aguilera et al.
The Journal of biological chemistry, 295(17), 5785-5794 (2020-03-15)
The Mycobacterium tuberculosis virulence factor EsxA and its chaperone EsxB are secreted as a heterodimer (EsxA:B) and are crucial for mycobacterial escape from phagosomes and cytosolic translocation. Current findings support the idea that for EsxA to interact with host membranes
Sean J Humphrey et al.
Nature biotechnology, 33(9), 990-995 (2015-08-19)
Mass spectrometry has enabled the study of cellular signaling on a systems-wide scale, through the quantification of post-translational modifications, such as protein phosphorylation. Here we describe EasyPhos, a scalable phosphoproteomics platform that now allows rapid quantification of hundreds of phosphoproteomes
Daniela M Remus et al.
Journal of bacteriology, 195(3), 502-509 (2012-11-24)
Sortases are transpeptidases that couple surface proteins to the peptidoglycan of Gram-positive bacteria, and several sortase-dependent proteins (SDPs) have been demonstrated to be crucial for the interactions of pathogenic and nonpathogenic bacteria with their hosts. Here, we studied the role
Pedro Farias et al.
Frontiers in microbiology, 12, 718963-718963 (2021-09-25)
Tellurium (Te) is a metalloid with scarce and scattered abundance but with an increased interest in human activity for its uses in emerging technologies. As is seen for other metals and metalloids, the result of mining activity and improper disposal
Michael P Smith et al.
The EMBO journal, 40(14), e107182-e107182 (2021-06-05)
Integration of signalling downstream of individual receptor tyrosine kinases (RTKs) is crucial to fine-tune cellular homeostasis during development and in pathological conditions, including breast cancer. However, how signalling integration is regulated and whether the endocytic fate of single receptors controls
William E Running et al.
Journal of proteome research, 6(1), 337-347 (2007-01-06)
Ribosomes from the Gram-negative alpha-proteobacterium Caulobacter crescentus were isolated using standard methods. Proteins were separated using a two-dimensional liquid chromatographic system that allowed the analysis of whole proteins by direct coupling to an ESI-QTOF mass spectrometer and of proteolytic digests
Ji-Young Youn et al.
Molecular cell, 69(3), 517-532 (2018-02-06)
mRNA processing, transport, translation, and ultimately degradation involve a series of dedicated protein complexes that often assemble into large membraneless structures such as stress granules (SGs) and processing bodies (PBs). Here, systematic in vivo proximity-dependent biotinylation (BioID) analysis of 119 human
Roger Moreno-Justicia et al.
STAR protocols, 3(1), 101135-101135 (2022-02-08)
The assembly of mitochondrial respiratory complexes into supercomplexes has significant implications for mitochondrial function. This protocol details mitochondrial isolation from mouse tissues and the use of blue native gel electrophoresis (BN-PAGE) to separate pre-identified mitochondrial supercomplexes into different gel bands. We
Agnieszka T Kawashima et al.
Molecular and cellular biology (2021-01-06)
PH domain Leucine-Rich Repeat Protein Phosphatase 1 (PHLPP1) is a tumor suppressor that directly dephosphorylates a wide array of substrates, most notably the pro-survival kinase Akt. However, little is known about the molecular mechanisms governing PHLPP1 itself. Here we report
Kalliopi Stratigi et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(13), E1577-E1586 (2015-03-15)
Physiological processes rely on the regulation of total mRNA levels in a cell. In diploid organisms, the transcriptional activation of one or both alleles of a gene may involve trans-allelic interactions that provide a tight spatial and temporal level of
Jeffrey Molendijk et al.
eLife, 11 (2022-12-07)
Improving muscle function has great potential to improve the quality of life. To identify novel regulators of skeletal muscle metabolism and function, we performed a proteomic analysis of gastrocnemius muscle from 73 genetically distinct inbred mouse strains, and integrated the
Matthew A Lauber et al.
Analytical chemistry, 82(18), 7736-7743 (2010-08-28)
A novel bifunctional thioimidate cross-linking reagent (diethyl suberthioimidate) that modifies amines without sacrificing their native basicity is developed. Intermolecular cross-linking of neurotensin and intramolecular cross-linking of cytochrome c under physiological conditions is investigated with this reagent. Because it does not
Lorenzo Bonaguro et al.
Nature immunology, 21(12), 1517-1527 (2020-11-11)
CRELD1 is a pivotal factor for heart development, the function of which is unknown in adult life. We here provide evidence that CRELD1 is an important gatekeeper of immune system homeostasis. Exploiting expression variance in large human cohorts contrasting individuals
Douglas E Vetter et al.
Methods in molecular biology (Clifton, N.J.), 493, 345-366 (2008-10-08)
Modern biologists have at their disposal a large array of techniques used to assess the existence and relative or absolute quantity of any molecule of interest in a sample. However, implementing most of these procedures can be a daunting task
Onrapak Reamtong et al.
Acta tropica, 202, 105247-105247 (2019-11-02)
Schistosoma mekongi is a causative agent of human schistosomiasis. There is limited knowledge of the molecular biology of S. mekongi and very few studies have examined drug targets, vaccine candidates and diagnostic biomarkers for S. mekongi. To explore the biology
Peter Karuso et al.
Journal of proteome research, 7(1), 361-366 (2007-12-07)
Ensuring that proteolytic digestions are complete before submitting samples for downstream proteomic analyses is important, as failure or partial digestion can waste valuable instrument time and make results difficult to interpret. Conversely, overdigestion can also be problematic, such as when
Eileen Ke et al.
Pancreas, 38(2), e33-e42 (2009-01-13)
There are currently no diagnostic indicators that are consistently reliable, obtainable, and conclusive for diagnosing and risk-stratifying pancreatic cysts. Proteomic analyses were performed to explore pancreatic cyst fluids to yield effective diagnostic biomarkers. We have prospectively recruited 20 research participants
Tamiza Nanji et al.
Autophagy, 13(12), 2018-2027 (2017-10-05)
Although the human ULK complex mediates phagophore initiation similar to the budding yeast Saccharomyces cerevisiae Atg1 complex, this complex contains ATG101 but not Atg29 and Atg31. Here, we analyzed the fission yeast Schizosaccharomyces pombe Atg1 complex, which has a subunit
Quantitative proteomics and phosphoproteomics of urinary extracellular vesicles define putative diagnostic biosignatures for Parkinson's disease.
Hadisurya, et al.
Communications medicine, 3, 64-64 (2023)
Guilherme Reis-de-Oliveira et al.
Methods in molecular biology (Clifton, N.J.), 2228, 341-352 (2021-05-06)
Here, we describe a proteomic pipeline to use a human microglial cell line as a biological model to study schizophrenia. In order to maximize the proteome coverage, we apply two-dimensional liquid chromatography coupled with ultra-definition MSE mass spectrometry (LC-UDMSE) using
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