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J P Casazza et al.
Alcohol and alcoholism (Oxford, Oxfordshire). Supplement, 1, 607-609 (1987-01-01)
Plasma 1,2-propanediol, D,L,-2,3-butanediol and 2,3-meso-butanediol were measured in 29 patients with biopsy proven alcoholic cirrhosis and 10 control subjects, none of whom had measurable blood alcohol levels. None of these compounds were present in control subjects at level above 5
Gen Lin Zhang et al.
Biotechnology letters, 34(8), 1519-1523 (2012-05-02)
The budC gene encoding the meso-2,3-BDH from Klebsiella pneumoniae XJ-Li was expressed in E. coli BL21 (DE3) pLys. Hypothetical amino acid sequence alignments revealed that the enzyme belongs to the short chain dehydrogenase/reductase family. After purification and refolding, the recombinant
Yanjie Zhang et al.
Bioresource technology, 130, 256-260 (2013-01-12)
A new strain, FMME044, exhibited a remarkable ability to synthesize acetoin and was identified as Bacillus amyloliquefaciens. The following characteristics of enzyme activity were found: 2,3-butanediol was reverse transformed to acetoin upon depletion of glucose; lower agitation speeds favored 2,3-butanediol
John Isaac Murray et al.
Molecular biology of the cell, 15(5), 2361-2374 (2004-03-09)
We used cDNA microarrays in a systematic study of the gene expression responses of HeLa cells and primary human lung fibroblasts to heat shock, endoplasmic reticulum stress, oxidative stress, and crowding. Hierarchical clustering of the data revealed groups of genes
Bram Vivijs et al.
International journal of food microbiology, 175, 36-44 (2014-02-18)
The mechanisms by which Enterobacteriaceae can survive or grow at low pH are of interest because members of this family are increasingly linked to problems of spoilage and foodborne infection related to mildly acidic foods. In this work, we investigated
Tao-Wei Yang et al.
Applied microbiology and biotechnology, 97(17), 7651-7658 (2013-06-26)
Cultivation in glycerol instead of sugars inhibits 2,3-butanediol (2,3-BD) production by Bacillus amyloliquefaciens. In this study, we report that B. amyloliquefaciens readily produces 2,3-BD from biodiesel-derived glycerol in the presence of beet molasses as a co-substrate. Unexpectedly, the molasses stimulated
V J Wroblewski
Biochemical pharmacology, 42(4), 889-897 (1991-07-25)
Examination of the disposition of proteins employing 125I-labeled tracers can be complicated by the in vivo deiodination of the tracer. The purpose of this study was to characterize the mechanism by which 125I-labeled proteins are deiodinated in vivo using 125I-human
Bioconversion of pentoses to 2,3-butanediol by Klebsiella pneumoniae.
N B Jansen et al.
Advances in biochemical engineering/biotechnology, 27, 85-99 (1983-01-01)
Safiyh Taghavi et al.
PloS one, 10(1), e0115455-e0115455 (2015-01-22)
Growth in sucrose medium was previously found to trigger the expression of functions involved in the plant associated life style of the endophytic bacterium Enterobacter sp. 638. Therefore, comparative transcriptome analysis between cultures grown in sucrose or lactate medium was
2,3-Butanediol in serum of alcoholics.
J Kuehnle et al.
Lancet (London, England), 2(8363), 1369-1370 (1983-12-10)
Borim Kim et al.
Journal of microbiology and biotechnology, 22(9), 1258-1263 (2012-07-21)
2,3-Butanediol (2,3-BD) is a major metabolite produced by Klebsiella pneumoniae KCTC2242, which is a important chemical with wide applications. Three genes important for 2,3-BD biosynthesis acetolactate decarboxylase (budA), acetolactate synthase (budB), and alcohol dehydrogenase (budC) were identified in K. pneumoniae
Y Yoshida
[Hokkaido igaku zasshi] The Hokkaido journal of medical science, 60(2), 183-194 (1985-03-01)
Methionine is a sulfur-containing essential amino acid and there are optic isomers of L- and D-type. It has been known that there are two metabolic pathways in methionine metabolism-the transsulfuration and the transamination. The purposes of the present investigation are
A Sakai et al.
Journal of biochemistry, 105(2), 275-280 (1989-02-01)
We have prepared a disulfide cross-linked hybrid consisting of human chorionic gonadotropin and the toxic ricin A chain, utilizing N-succinimidyl-3-(2-pyridyldithio)-propionate. The purified hybrid exhibited cytotoxicity toward rat testis Leydig cells due to inhibition of protein synthesis within the cells, although
C Hahn et al.
Journal of lipid research, 36(9), 2059-2066 (1995-09-01)
The serum concentration of 7 alpha-hydroxycholesterol as an indicator of total bile acid synthesis was investigated under different experimental conditions in humans. 7 alpha-Hydroxycholesterol was measured by gas-liquid chromatography-mass spectrometry, using [2H7]7 alpha-hydroxycholesterol and/or 5 alpha-cholestane-3 beta, 6 beta-diol as
C A Cooper et al.
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 143(3), 292-298 (2006-01-25)
In the marine teleost intestine the secretion of bicarbonate increases pH of the lumen (pH 8.4 -9.0) and importantly reduces Ca2+ and Mg2+ concentrations by the formation of insoluble divalent ion carbonates. The alkaline intestinal environment could potentially also cause
B Z S Paul et al.
Journal of thrombosis and haemostasis : JTH, 1(4), 814-820 (2003-07-23)
The integrin alphavbeta3 mediates platelet adhesion to the matrix protein osteopontin and likely is the predominant integrin mediating platelet adhesion to the matrix protein vitronectin. To address the mechanism that regulates alphavbeta3 activity in platelets, we measured the effect of
Simultaneous determination of plasma phenethylamine, phenylethanolamine, tyramine and octopamine by high-performance liquid chromatography using derivatization with fluorescamine.
T Yonekura et al.
Journal of chromatography, 427(2), 320-325 (1988-06-03)
Luc A Cynober
Nutrition (Burbank, Los Angeles County, Calif.), 18(9), 761-766 (2002-09-26)
The plasma concentration of an amino acid (AA) is the result of its rates of appearance (Ra) in and disappearance (Rd) from plasma. As for most nutrients, AA Ra and Rd are tightly regulated and at the postabsorptive state Ra
Sang Heum Shin et al.
Journal of bacteriology, 194(10), 2736-2737 (2012-04-27)
Here we report the full genome sequence of Klebsiella pneumoniae KCTC 2242,consisting of a 5.26-Mb chromosome (57.6% GC%; 5,035 genes [4,923 encoding known proteins, 112 RNA genes]) and a 202-kb plasmid (50.2% GC%; 229 genes [229 encoding known proteins]).
V Walker et al.
Clinical chemistry, 35(7), 1460-1466 (1989-07-01)
Using analytical procedures that are widely used by laboratories investigating metabolic disorders, we investigated urinary organic acid excretion by premature neonates who were receiving the usual clinical care. Our purpose was to provide a basis for the diagnosis of inherited
Chiam Yu Ng et al.
Microbial cell factories, 11, 68-68 (2012-05-30)
2,3-Butanediol is a chemical compound of increasing interest due to its wide applications. It can be synthesized via mixed acid fermentation of pathogenic bacteria such as Enterobacter aerogenes and Klebsiella oxytoca. The non-pathogenic Saccharomyces cerevisiae possesses three different 2,3-butanediol biosynthetic
Xiao-Jun Ji et al.
Biotechnology advances, 29(3), 351-364 (2011-01-29)
2,3-butanediol is a promising bulk chemical due to its extensive industry applications. The state-of-the-art nature of microbial 2,3-butanediol production is reviewed in this paper. Various strategies for efficient and economical microbial 2,3-butanediol production, including strain improvement, substrate alternation, and process
Taowei Yang et al.
World journal of microbiology & biotechnology, 28(4), 1563-1574 (2012-07-19)
The effects of culture conditions on 2,3-butanediol (2,3-BD) production and its possible scale-up have been studied. A newly isolated Bacillus amyloliquefaciens B10-127, belonged to GRAS microorganisms and showed a remarkable 2,3-BD producing potency, was used for this experiment. Corn steep
Natalia Malfanova et al.
Archives of microbiology, 195(1), 9-17 (2012-09-08)
Twenty endophytic bacteria were isolated from surface-sterilized stems and roots of cucumber plants. After removal of potential siblings and human pathogens, the remaining seven strains were identified based on their 16S rDNA as Pseudomonas fluorescens (2 strains) and P. putida
Sung-Hyuk Han et al.
New biotechnology, 30(2), 166-172 (2012-09-20)
2,3-Butanediol (2,3-BDO) is a value-added chemical with great potential for the industrial production of synthetic rubber, plastic and solvent. For microbial production of 2,3-BDO, in this study, Klebsiella oxytoca NBRF4 was constructed by chemical mutation and screening against NaBr, NaBrO(3)
Norihiro Kobayashi et al.
Steroids, 67(10), 827-833 (2002-09-17)
Urinary levels of sulfated metabolites of lithocholic acid (LCA) are expected to be a useful index of liver function. Thus, a sensitive, specific, and feasible enzyme-linked immunosorbent assay (ELISA) of these sulfated LCA metabolites (LCA-Suls) should be established. A newly
A V Moshkin
Laboratornoe delo, (2)(2), 48-52 (1989-01-01)
Stabilization with dithiothreitol, together with optimization of Mg2+ and EDTA concentrations in the reaction mixture and storage of the CSF samples for 24 hrs at +4 degrees C, has been carried out for the first time to improve the sensitivity
Ailong Wang et al.
PloS one, 7(7), e40442-e40442 (2012-07-14)
2,3-Butanediol (BD) is considered as one of the key platform chemicals used in a variety of industrial applications. It is crucial to find an efficient sugar-utilizing strain and feasible carbon source for the economical production of BD. Efficient BD production
Muktak Aklujkar et al.
BMC genomics, 13, 690-690 (2012-12-12)
The bacterium Pelobacter carbinolicus is able to grow by fermentation, syntrophic hydrogen/formate transfer, or electron transfer to sulfur from short-chain alcohols, hydrogen or formate; it does not oxidize acetate and is not known to ferment any sugars or grow autotrophically.
B Marescau et al.
Metabolism: clinical and experimental, 44(5), 584-588 (1995-05-01)
To investigate the metabolic relationship between urea and guanidinosuccinic acid (GSA), we determined the levels of the guanidino compounds, including GSA, and urea in serum and urine of cirrhotic patients. Linear correlation studies between serum urea and GSA levels were
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