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E Celińska et al.
Biotechnology advances, 27(6), 715-725 (2009-05-16)
Biotechnological production of 2,3-butanediol (hereafter referred to as 2,3-BD) from wastes and excessive biomass is a promising and attractive alternative for traditional chemical synthesis. In the face of scarcity of fossil fuel supplies the bio-based process is receiving a significant
Sung-Geun Jung et al.
Applied microbiology and biotechnology, 97(5), 1997-2007 (2012-07-27)
Klebsiella species are the most extensively studied among a number of 2,3-butanediol (2,3-BDO)-producing microorganisms. The ability to metabolize a wide variety of substrates together with the ease of cultivation made this microorganisms particularly promising for the application in industrial-scale production
Ailong Wang et al.
PloS one, 7(7), e40442-e40442 (2012-07-14)
2,3-Butanediol (BD) is considered as one of the key platform chemicals used in a variety of industrial applications. It is crucial to find an efficient sugar-utilizing strain and feasible carbon source for the economical production of BD. Efficient BD production
Xiao-Jun Ji et al.
Biotechnology advances, 29(3), 351-364 (2011-01-29)
2,3-butanediol is a promising bulk chemical due to its extensive industry applications. The state-of-the-art nature of microbial 2,3-butanediol production is reviewed in this paper. Various strategies for efficient and economical microbial 2,3-butanediol production, including strain improvement, substrate alternation, and process
Thomas Häßler et al.
Bioresource technology, 124, 237-244 (2012-09-20)
Fed-batch fermentations for the production of 2,3-butanediol (BDL) with Paenibacillus polymyxa DSM 365 were investigated in 2-L-fermenters. A suitable micro-aerobic set-up enabled high product selectivity of up to 98% R,R-BDL towards meso-BDL and acetoin. Up to 111 g L(-1)R,R-BDL within
Magalie Celton et al.
Metabolic engineering, 14(4), 366-379 (2012-06-20)
Controlling the amounts of redox cofactors to manipulate metabolic fluxes is emerging as a useful approach to optimizing byproduct yields in yeast biotechnological processes. Redox cofactors are extensively interconnected metabolites, so predicting metabolite patterns is challenging and requires in-depth knowledge
Yuzhen Zhang et al.
Bioresource technology, 121, 100-104 (2012-08-04)
Pretreatment is necessary for lignocellulose to achieve a highly efficient enzymatic hydrolysis and fermentation. However, coincident with pretreatment, compounds inhibiting microorganism growth are formed. Some tissues or cells, such as thin-walled cells that easily hydrolyze, will be excessively degraded because
Chiam Yu Ng et al.
Microbial cell factories, 11, 68-68 (2012-05-30)
2,3-Butanediol is a chemical compound of increasing interest due to its wide applications. It can be synthesized via mixed acid fermentation of pathogenic bacteria such as Enterobacter aerogenes and Klebsiella oxytoca. The non-pathogenic Saccharomyces cerevisiae possesses three different 2,3-butanediol biosynthetic
Taowei Yang et al.
World journal of microbiology & biotechnology, 28(4), 1563-1574 (2012-07-19)
The effects of culture conditions on 2,3-butanediol (2,3-BD) production and its possible scale-up have been studied. A newly isolated Bacillus amyloliquefaciens B10-127, belonged to GRAS microorganisms and showed a remarkable 2,3-BD producing potency, was used for this experiment. Corn steep
A V Moshkin
Laboratornoe delo, (2)(2), 48-52 (1989-01-01)
Stabilization with dithiothreitol, together with optimization of Mg2+ and EDTA concentrations in the reaction mixture and storage of the CSF samples for 24 hrs at +4 degrees C, has been carried out for the first time to improve the sensitivity
Norihiro Kobayashi et al.
Steroids, 67(10), 827-833 (2002-09-17)
Urinary levels of sulfated metabolites of lithocholic acid (LCA) are expected to be a useful index of liver function. Thus, a sensitive, specific, and feasible enzyme-linked immunosorbent assay (ELISA) of these sulfated LCA metabolites (LCA-Suls) should be established. A newly
Muktak Aklujkar et al.
BMC genomics, 13, 690-690 (2012-12-12)
The bacterium Pelobacter carbinolicus is able to grow by fermentation, syntrophic hydrogen/formate transfer, or electron transfer to sulfur from short-chain alcohols, hydrogen or formate; it does not oxidize acetate and is not known to ferment any sugars or grow autotrophically.
B Marescau et al.
Metabolism: clinical and experimental, 44(5), 584-588 (1995-05-01)
To investigate the metabolic relationship between urea and guanidinosuccinic acid (GSA), we determined the levels of the guanidino compounds, including GSA, and urea in serum and urine of cirrhotic patients. Linear correlation studies between serum urea and GSA levels were
V Walker et al.
Clinical chemistry, 35(7), 1460-1466 (1989-07-01)
Using analytical procedures that are widely used by laboratories investigating metabolic disorders, we investigated urinary organic acid excretion by premature neonates who were receiving the usual clinical care. Our purpose was to provide a basis for the diagnosis of inherited
Natalia Malfanova et al.
Archives of microbiology, 195(1), 9-17 (2012-09-08)
Twenty endophytic bacteria were isolated from surface-sterilized stems and roots of cucumber plants. After removal of potential siblings and human pathogens, the remaining seven strains were identified based on their 16S rDNA as Pseudomonas fluorescens (2 strains) and P. putida
Sung-Hyuk Han et al.
New biotechnology, 30(2), 166-172 (2012-09-20)
2,3-Butanediol (2,3-BDO) is a value-added chemical with great potential for the industrial production of synthetic rubber, plastic and solvent. For microbial production of 2,3-BDO, in this study, Klebsiella oxytoca NBRF4 was constructed by chemical mutation and screening against NaBr, NaBrO(3)
John Isaac Murray et al.
Molecular biology of the cell, 15(5), 2361-2374 (2004-03-09)
We used cDNA microarrays in a systematic study of the gene expression responses of HeLa cells and primary human lung fibroblasts to heat shock, endoplasmic reticulum stress, oxidative stress, and crowding. Hierarchical clustering of the data revealed groups of genes
Safiyh Taghavi et al.
PloS one, 10(1), e0115455-e0115455 (2015-01-22)
Growth in sucrose medium was previously found to trigger the expression of functions involved in the plant associated life style of the endophytic bacterium Enterobacter sp. 638. Therefore, comparative transcriptome analysis between cultures grown in sucrose or lactate medium was
Yanjie Zhang et al.
Bioresource technology, 130, 256-260 (2013-01-12)
A new strain, FMME044, exhibited a remarkable ability to synthesize acetoin and was identified as Bacillus amyloliquefaciens. The following characteristics of enzyme activity were found: 2,3-butanediol was reverse transformed to acetoin upon depletion of glucose; lower agitation speeds favored 2,3-butanediol
Bram Vivijs et al.
International journal of food microbiology, 175, 36-44 (2014-02-18)
The mechanisms by which Enterobacteriaceae can survive or grow at low pH are of interest because members of this family are increasingly linked to problems of spoilage and foodborne infection related to mildly acidic foods. In this work, we investigated
Lixiang Li et al.
Journal of bacteriology, 194(15), 4133-4134 (2012-07-21)
Both Bacillus licheniformis strains 10-1-A and 5-2-D are efficient producers of 2,3-butanediol. Here we present 4.3-Mb and 4.2-Mb assemblies of their genomes. The key genes for the regulation and metabolism of 2,3-butanediol production were annotated, which may provide further insights
Laurent Imbert et al.
Journal of analytical toxicology, 38(9), 676-680 (2014-09-05)
A liquid chromatography coupled with electrospray tandem mass spectrometry method was developed for the analysis of ethylene glycol, diethylene glycol, triethylene glycol, 1,4-butanediol, 1,2-butanediol, 2,3-butanediol, 1,2-propanediol and 1,3-propanediol, in serum after a Schotten-Baumann derivatization by benzoyl chloride. Usual validation parameters
Hsin-Chih Lai et al.
Journal of leukocyte biology, 92(4), 807-814 (2012-07-18)
The natural compound 2,3-BTD has diverse physiological effects in a range of organisms, including acting as a detoxifying product of liver alcohol metabolism in humans and ameliorating endotoxin-induced acute lung injury in rats. In this study, we reveal that 2,3-BTD
N Tamasawa et al.
Lipids, 28(9), 833-836 (1993-09-01)
An analytical method for the determination of cholesterol sulfate (CS) in plasma using gas-liquid chromatography was developed. We measured plasma CS concentrations in patients with liver cirrhosis and hypercholesterolemia as examples of disorders that involve aberrations in cholesterol metabolism. Patients
Sang Heum Shin et al.
Journal of bacteriology, 194(10), 2736-2737 (2012-04-27)
Here we report the full genome sequence of Klebsiella pneumoniae KCTC 2242,consisting of a 5.26-Mb chromosome (57.6% GC%; 5,035 genes [4,923 encoding known proteins, 112 RNA genes]) and a 202-kb plasmid (50.2% GC%; 229 genes [229 encoding known proteins]).
Simultaneous determination of plasma phenethylamine, phenylethanolamine, tyramine and octopamine by high-performance liquid chromatography using derivatization with fluorescamine.
T Yonekura et al.
Journal of chromatography, 427(2), 320-325 (1988-06-03)
Natsuo Ueda et al.
Neuropharmacology, 48(8), 1079-1085 (2005-05-25)
It is widely accepted that fatty acid amide hydrolase (FAAH) plays a central role in the hydrolysis of anandamide. However, we found a second N-acylethanolamine hydrolase in animal tissues which hydrolyzed anandamide at acidic pH. This "acid amidase" was first
C Hahn et al.
Journal of lipid research, 36(9), 2059-2066 (1995-09-01)
The serum concentration of 7 alpha-hydroxycholesterol as an indicator of total bile acid synthesis was investigated under different experimental conditions in humans. 7 alpha-Hydroxycholesterol was measured by gas-liquid chromatography-mass spectrometry, using [2H7]7 alpha-hydroxycholesterol and/or 5 alpha-cholestane-3 beta, 6 beta-diol as
2,3-Butanediol in serum of alcoholics.
J Kuehnle et al.
Lancet (London, England), 2(8363), 1369-1370 (1983-12-10)
V J Wroblewski
Biochemical pharmacology, 42(4), 889-897 (1991-07-25)
Examination of the disposition of proteins employing 125I-labeled tracers can be complicated by the in vivo deiodination of the tracer. The purpose of this study was to characterize the mechanism by which 125I-labeled proteins are deiodinated in vivo using 125I-human
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