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Marta Toth et al.
Methods in molecular biology (Clifton, N.J.), 878, 121-135 (2012-06-08)
Gelatin zymography is a simple yet powerful method to detect proteolytic enzymes capable of degrading gelatin from various biological sources. It is particularly useful for the assessment of two key members of the matrix metalloproteinase family, MMP-2 (gelatinase A) and
Jameel A Baig et al.
Journal of AOAC International, 95(6), 1755-1760 (2013-03-05)
A method has been developed for the determination of arsenic (As) in pharmaceutical and scalp hair samples of kidney failure patients by cloud point extraction (CPE). The scalp hair samples were subjected to microwave-assisted digestion in nitric acid-hydrogen peroxide (2
Hongbing Yang et al.
Journal of immunological methods, 391(1-2), 174-178 (2013-03-19)
The capacity of CD8+ T cells to inhibit HIV-1 replication in vitro strongly correlates with virus control in vivo. Post-hoc evaluations of HIV-1 vaccine candidates suggest that this immunological parameter is a promising benchmark of vaccine efficacy. Large-scale analysis of
Russell M Morphew et al.
Veterinary parasitology, 191(3-4), 379-385 (2012-10-02)
Fasciola hepatica NP-40 released protein extract (FhNPE) exhibits potent Th1 immunosuppressive properties in vitro and in vivo. However, the protein composition of this active fraction, responsible for Th1 immune modulatory activity, has yet to be resolved. Therefore, FhNPE, a Nonidet
Mustafa Soylak et al.
Journal of AOAC International, 95(6), 1797-1802 (2013-03-05)
A cloud point extraction procedure for the preconcentration of Pb2+ in various samples following complexation with 2,2'-(1E,1'E)-1,1'-(2,2'-azanediylbis(ethane-2,1-diyl)bis(azan-1-yl-1-ylidene)) bis(ethan-1-yl-1-ylidene)diphenol in Triton X-114 after centrifugation is reported. A 0.5 mL portion of methanol acidified with 1.0 M HNO3 was added to the
Mohammad Eftekhari et al.
Environmental monitoring and assessment, 187(1), 4129-4129 (2014-11-19)
Vortex-assisted surfactant-enhanced emulsification microextraction based on solidification of floating organic drop (VASEME-SFO) was used for preconcentration and speciation of antimony (ΙΙΙ, V) followed by electrothermal atomic absorption spectrometry (ETAAS). In this procedure, Triton X-114 was used as emulsifier and 1-undecanol
Halil İbrahim Ulusoy et al.
Talanta, 88, 516-523 (2012-01-24)
A new micelle-mediated separation and preconcentration method was developed for ultra-trace quantities of mercury ions prior to spectrophotometric determination. The method is based on cloud point extraction (CPE) of Hg(II) ions with polyethylene glycol tert-octylphenyl ether (Triton X-114) in the
Wuttikon Rodkvamtook et al.
The American journal of tropical medicine and hygiene, 92(5), 967-971 (2015-03-25)
We developed a rapid dot-enzyme-linked immunosorbent assay (dot-ELISA) using the combination of recombinant 56-kDa protein antigens that exhibited broad reactivity with serum antibodies against the four most prevalent strains (Karp, Kato, Gilliam, and TA763) of Orientia tsutsugamushi. The assay is
D V Krishna Pantakani et al.
PloS one, 6(12), e29584-e29584 (2012-01-05)
ZFYVE27 (Protrudin) was originally identified as an interacting partner of spastin, which is most frequently mutated in hereditary spastic paraplegia. ZFYVE27 is a novel member of FYVE family, which is implicated in the formation of neurite extensions by promoting directional
S Okonogi et al.
Drug discoveries & therapeutics, 6(5), 249-255 (2012-12-12)
The aim of the present study was to enhance the cholinesterase inhibitory activity of Zingiber cassumunar (ZC) oil using a microemulsion (ME) technique. Pseudoternary phase diagrams of the oil, water, and surfactant/co-surfactant mixture were constructed using a water titration method.
Rui Ma et al.
Analytical biochemistry, 424(2), 124-126 (2012-03-01)
Triton X-114 temperature transition extraction has been considered to be a simple and cost-effective strategy to eliminate endotoxin from plasmid preparations. However, a repeated cooling-heating process may promote the degradation of plasmid DNA. Based on the finding that the cloud
Lingling Zhao et al.
Journal of hazardous materials, 239-240, 206-212 (2012-09-22)
A dual-cloud point extraction (d-CPE) procedure has been developed for simultaneous pre-concentration and separation of heavy metal ions (Cd2+, Co2+, Ni2+, Pb2+, Zn2+, and Cu2+ ion) in water samples by inductively coupled plasma optical emission spectrometry (ICP-OES). The procedure is
Georg Hartmann et al.
Analytica chimica acta, 761, 27-33 (2013-01-15)
The determination of metallic nanoparticles in environmental samples requires sample pretreatment that ideally combines pre-concentration and species selectivity. With cloud point extraction (CPE) using the surfactant Triton X-114 we present a simple and cost effective separation technique that meets both
Jaqueline Bianchi et al.
Ecotoxicology and environmental safety, 120, 174-183 (2015-06-16)
The insecticide imidacloprid and the herbicide sulfentrazone are two different classes of pesticides that are used for pest control in sugarcane agriculture. To evaluate the genotoxic potential of low concentrations of these two pesticides alone and in mixture, the comet
Zonghao Li et al.
Water science and technology : a journal of the International Association on Water Pollution Research, 67(2), 247-253 (2012-11-22)
A new, simple and rapid method based on dispersive liquid-liquid microextraction (DLLME) was developed for extracting and preconcentrating copper (Cu), nickel (Ni), lead (Pb) and cadmium (Cd) in water samples prior to flame atomic absorption spectrometry (FAAS) analysis. 1-(2-thiazolylazo)-naphthol (TAN)
Effect of surfactant addition on the MWNT suspenzions stability in organic solvents
Gigiberiya VA et al.
Journal of Separation Science (2011)
Trícia Maria Ferreira de Sousa Oliveira et al.
Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria, 24(2), 220-222 (2015-07-15)
The relevance of the dog as a source of visceral leishmaniasis infection is known, but the role of cats as reservoir hosts for leishmaniasis is not yet fully clear. This study assessed the efficacy of conjunctival swab PCR (CS-PCR) in
Surface tension-dependent surfactant toxicity on the green peach aphid, Myzus persicae (Sulzer)(Hemiptera: Aphididae)
IMAI, et al.
Journal of Separation Science, 29(3), 389-393 (1994)
John E Bradley et al.
Laboratory investigation; a journal of technical methods and pathology, 93(3), 365-374 (2013-01-30)
Thymocyte differentiation antigen-1 (Thy-1) is a glycosylphosphatidylinositol (GPI)-linked cell surface glycoprotein expressed on numerous cell types, which regulates signals affecting cell adhesion, migration, differentiation, and survival. In addition, Thy-1 has been detected in the serum, cerebral spinal fluid, wound fluid
R Lappe et al.
Natural product research, 26(23), 2259-2265 (2012-01-21)
Cerein 8A is an antimicrobial peptide with potential application against food spoilage and pathogenic bacteria. The partitioning of cerein 8A was investigated in two liquid-liquid extraction systems that are considered promising for bioseparation and purification purposes. Aqueous two-phase systems (ATPSs)
Hamta Shoaee et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 98, 70-75 (2012-09-18)
A cloud-point extraction process coupled to ICP-OES by using 3-nitro benzaldehyde thiosemicarbazone (3-NBT) as complexing agent was developed for the simultaneous preconcentration and determination of copper and mercury in water samples. The variables affecting the complexation and extraction steps were
Ebru Ates et al.
Journal of AOAC International, 94(6), 1853-1862 (2012-02-11)
A cloud point extraction method is reported using LC/MS for the determination of regulated water-soluble food colors (Allura Red, Sunset Yellow, erythrosine, and tartrazine) and banned fat-soluble synthetic azo dyes (Sudan I, II, III, and IV; Red B; 7B; Black
Shikha Gupta et al.
Journal of pharmaceutical and biomedical analysis, 66, 33-39 (2012-04-03)
A new validated high-performance thin-layer chromatographic (HPTLC) method has been developed for the simultaneous quantitation of four antipsychotic indole alkaloids (IAs), reserpiline (RP, 1), α-yohimbine (YH, 2), isoreserpiline (IRP, 3) and 10-methoxy tetrahydroalstonine (MTHA, 4) as markers in the leaves
Qi Zhao et al.
Journal of chromatographic science, 51(4), 341-348 (2012-09-15)
A method based on cloud point extraction (CPE) coupled with high-performance liquid chromatography separation and ultraviolet (UV) detection was developed to determine andrographolide and dehydroandrographolide in human plasma. The nonionic surfactant Triton X-114 was chosen as the extraction medium. Variable
Amr Ali Mohamed et al.
Journal of colloid and interface science, 381(1), 198-201 (2012-06-19)
Uniform fully dispersed particles of flufenamic acid, a widely used anti-inflammatory drug, were prepared by two different methods. In the first one, the drug solution in organic solvents was added to a non-solvent (water or aqueous solutions of stabilizers); while
Friedrich P Thinnes et al.
Molecular genetics and metabolism, 107(3), 632-633 (2012-09-25)
There is indication that human type-1 VDAC/Porin31HL complexes, when purified from highly enriched cell membrane preparations of human B-lymphocytes by classical ion-exchange chromatography in the detergent Nonidet P40, rest in fully closed state, its N-terminus being accessible for mAbs. Cholesterol
Ana C A M Pajuaba et al.
Proteomics, 12(6), 820-831 (2012-04-28)
Brucella abortus is a Gram-negative intracellular bacterium that causes infectious abortion in food-producing animals and chronic infection in humans. This study aimed to characterize a B. abortus S19 antigen preparation obtained by Triton X-114 (TX-114) extraction through immunoproteomics to differentiate
Qingwen Deng et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 104, 64-69 (2012-12-26)
In this work, dispersive liquid-liquid microextraction (DLLME) was improved and the preconcentration method named as surfactant assisted emulsification dispersive liquid-liquid microextraction (SAE-DLLME) was established for ultra trace nickel preconcentration and spectrophotometric determination. Non-ionic surfactant Triton X-100 (TX-100) was used as
Chunyapuk Kukusamude et al.
Journal of chromatography. A, 1351, 110-114 (2014-06-09)
Sample clean-up by cloud point phase separation and analysis by capillary electrophoresis with stacking was developed for quaternary ammonium herbicides (i.e., paraquat and diquat) in milk. For sample clean-up, a mixture of 845μL of milk sample, 5μL of 100mM phosphoric
Effect of surfactant addition on the MWNT suspenzions stability in organic solvents
Gigiberiya VA et al.
Journal of Separation Science (2011)
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